ID   ICIB_HORVU              Reviewed;          83 AA.
AC   P16063;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Subtilisin-chymotrypsin inhibitor CI-1B;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   AGRICOLA=IND92000036; DOI=10.1007/BF00033607;
RA   Williamson M.S., Forde J., Kreis M.;
RT   "Molecular cloning of two isoinhibitor forms of chymotrypsin inhibitor 1
RT   (CI-1) from barley endosperm and their expression in normal and mutant
RT   barleys.";
RL   Plant Mol. Biol. 10:521-535(1988).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8031854; DOI=10.1016/0167-4889(94)90167-8;
RA   Greagg M.A., Brauer A.B., Leatherbarrow R.J.;
RT   "Expression and kinetic characterization of barley chymotrypsin inhibitors
RT   1a and 1b.";
RL   Biochim. Biophys. Acta 1222:179-186(1994).
CC   -!- FUNCTION: Inhibits both subtilisin and chymotrypsin.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   EMBL; M21401; AAA32946.1; -; mRNA.
DR   PIR; JA0182; JA0182.
DR   AlphaFoldDB; P16063; -.
DR   SMR; P16063; -.
DR   MEROPS; I13.005; -.
DR   EnsemblPlants; HORVU.MOREX.r2.1HG0009490.1; HORVU.MOREX.r2.1HG0009490.1.CDS.1; HORVU.MOREX.r2.1HG0009490.
DR   EnsemblPlants; HORVU.MOREX.r2.1HG0009490.1.mrna1; HORVU.MOREX.r2.1HG0009490.1.mrna1.cds1; HORVU.MOREX.r2.1HG0009490.1.
DR   Gramene; HORVU.MOREX.r2.1HG0009490.1; HORVU.MOREX.r2.1HG0009490.1.CDS.1; HORVU.MOREX.r2.1HG0009490.
DR   Gramene; HORVU.MOREX.r2.1HG0009490.1.mrna1; HORVU.MOREX.r2.1HG0009490.1.mrna1.cds1; HORVU.MOREX.r2.1HG0009490.1.
DR   OMA; MRSMEGS; -.
DR   ExpressionAtlas; P16063; differential.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   PRINTS; PR00292; POTATOINHBTR.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Protease inhibitor; Serine protease inhibitor.
FT   CHAIN           1..83
FT                   /note="Subtilisin-chymotrypsin inhibitor CI-1B"
FT                   /id="PRO_0000217648"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            34..35
FT                   /note="Reactive bond for subtilisin"
FT                   /evidence="ECO:0000250"
FT   SITE            63..64
FT                   /note="Reactive bond for chymotrypsin and subtilisin"
SQ   SEQUENCE   83 AA;  8963 MW;  A98827155969FFF5 CRC64;
     MRSMEGSVPK YPEPTEGSIG ASGAKRSWPE VVGMSAEKAK EIILRDKPDA QIEVIPVDAM
     VPLDFNPNRI FILVAVARTP TVG