ID   ICK8_TRILK              Reviewed;         119 AA.
AC   W4VSI8;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=Toxin ICK-8;
DE   Flags: Precursor;
OS   Trittame loki (Brush-footed trapdoor spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Barychelidae; Trittame.
OX   NCBI_TaxID=1295018;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=24351713; DOI=10.3390/toxins5122488;
RA   Undheim E.A., Sunagar K., Herzig V., Kely L., Low D.H., Jackson T.N.,
RA   Jones A., Kurniawan N., King G.F., Ali S.A., Antunes A., Ruder T.,
RA   Fry B.G.;
RT   "A proteomics and transcriptomics investigation of the venom from the
RT   barychelid spider Trittame loki (brush-foot trapdoor).";
RL   Toxins 5:2488-2503(2013).
CC   -!- FUNCTION: Ion channel inhibitor. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 25 family. ICK-8 subfamily.
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DR   EMBL; GAQE01000011; JAB84543.1; -; Transcribed_RNA.
DR   AlphaFoldDB; W4VSI8; -.
DR   SMR; W4VSI8; -.
DR   ArachnoServer; AS001781; U1-barytoxin-Tl1c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..119
FT                   /note="Toxin ICK-8"
FT                   /id="PRO_0000429215"
FT   DISULFID        59..74
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..80
FT                   /evidence="ECO:0000250"
FT   DISULFID        71..116
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..87
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   119 AA;  13078 MW;  9FC6113333E4BAE6 CRC64;
     MMKLYSLVII ATLAAAAFAA TSEEISAAVS EIISQHQEDL ERYAKIVERG EEPKKYIRCS
     KQLGQSCYLN CECCGASAVC EDIKYICKDK VSDNSILDAM GKAWNAVGNS ISRYYCSAE