ID   ICLN_XENLA              Reviewed;         241 AA.
AC   P54106;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Methylosome subunit pICln;
DE   AltName: Full=Chloride conductance regulatory protein ICln;
DE            Short=I(Cln);
GN   Name=clns1a; Synonyms=icln;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RX   PubMed=8313467; DOI=10.1016/0092-8674(94)90109-0;
RA   Krapivinsky G.B., Ackerman M.J., Gordon E.A., Krapivinsky L.D.,
RA   Clapham D.E.;
RT   "Molecular characterization of a swelling-induced chloride conductance
RT   regulatory protein, pICln.";
RL   Cell 76:439-448(1994).
CC   -!- FUNCTION: Involved in both the assembly of spliceosomal snRNPs and the
CC       methylation of Sm proteins (By similarity). Chaperone that regulates
CC       the assembly of spliceosomal U1, U2, U4 and U5 small nuclear
CC       ribonucleoproteins (snRNPs), the building blocks of the spliceosome,
CC       and thereby plays an important role in the splicing of cellular pre-
CC       mRNAs (By similarity). Most spliceosomal snRNPs contain a common set of
CC       Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that
CC       assemble in a heptameric protein ring on the Sm site of the small
CC       nuclear RNA to form the core snRNP (Sm core) (By similarity). In the
CC       cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are
CC       trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that
CC       controls the assembly of the core snRNP (By similarity). Dissociation
CC       by the SMN complex of CLNS1A from the trapped Sm proteins and their
CC       transfer to an SMN-Sm complex triggers the assembly of core snRNPs and
CC       their transport to the nucleus (By similarity).
CC       {ECO:0000250|UniProtKB:P54105}.
CC   -!- SUBUNIT: Component of the methylosome, a 20S complex containing at
CC       least clns1a/picln, prmt5/skb1 and wdr77/mep50; may mediate snrpd1 and
CC       snrpd3 methylation. Forms a 6S pICln-Sm complex composed of
CC       clns1a/picln, snrpd1, snrpd2, snrpe, snrpf and snrpg; ring-like
CC       structure where clns1a/pICln mimics additional Sm proteins and which is
CC       unable to assemble into the core snRNP. {ECO:0000250|UniProtKB:P54105}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P54105}. Nucleus {ECO:0000250|UniProtKB:P54105}.
CC       Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P54105}.
CC   -!- SIMILARITY: Belongs to the pICln (TC 1.A.47) family. {ECO:0000305}.
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DR   EMBL; L26449; AAC38009.1; -; mRNA.
DR   PIR; A53014; A53014.
DR   RefSeq; NP_001081766.1; NM_001088297.1.
DR   AlphaFoldDB; P54106; -.
DR   SMR; P54106; -.
DR   MaxQB; P54106; -.
DR   DNASU; 398038; -.
DR   GeneID; 398038; -.
DR   KEGG; xla:398038; -.
DR   CTD; 398038; -.
DR   Xenbase; XB-GENE-17346342; clns1a.S.
DR   OrthoDB; 1508796at2759; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 398038; Expressed in blastula and 19 other tissues.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0034709; C:methylosome; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0034715; C:pICln-Sm protein complex; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0006884; P:cell volume homeostasis; IEA:InterPro.
DR   GO; GO:0006821; P:chloride transport; IEA:InterPro.
DR   GO; GO:0000387; P:spliceosomal snRNP assembly; ISS:UniProtKB.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR003521; ICln.
DR   InterPro; IPR039924; ICln/Lot5/Saf5.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   PANTHER; PTHR21399; PTHR21399; 1.
DR   Pfam; PF03517; Voldacs; 1.
DR   PRINTS; PR01348; ICLNCHANNEL.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..241
FT                   /note="Methylosome subunit pICln"
FT                   /id="PRO_0000185158"
FT   REGION          88..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..112
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   241 AA;  26446 MW;  4E62C01EDE0E224E CRC64;
     MNLLSSFPPP ADGVRRLQPG TEAVVGGRGL GPGTLYIAES RLSWLNGSGL GFSLEYPSIS
     LHAISRDTAA YPEEHLYVMV NSKLADKEDK EAHMADQEEE ESEDDDDDEE PITEIRFVPG
     EKSDLGEMFS AMCDCQALHP DPEDADSDDD YEGDEYDVEA HEQGQVDVPT FYTYEEGLSH
     LTTEGQATLE RLENMLSNSI GNQHTMAGVR TEGPALEPED GMDVENTQTV AGQFEDADVD
     H