ICOS_RAT
ID ICOS_RAT Reviewed; 200 AA.
AC Q9R1T7; Q9WVR9;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Inducible T-cell costimulator;
DE AltName: Full=Activation-inducible lymphocyte immunomediatory molecule;
DE AltName: CD_antigen=CD278;
DE Flags: Precursor;
GN Name=Icos; Synonyms=Ailim;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 21-40,
RP FUNCTION, SUBUNIT, INDUCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Spleen;
RX PubMed=11006126; DOI=10.1006/bbrc.2000.3466;
RA Tezuka K., Tsuji T., Hirano D., Tamatani T., Sakamaki K., Kobayashi Y.,
RA Kamada M.;
RT "Identification and characterization of rat AILIM/ICOS, a novel T-cell
RT costimulatory molecule, related to the CD28/CTLA4 family.";
RL Biochem. Biophys. Res. Commun. 276:335-345(2000).
CC -!- FUNCTION: Enhances all basic T-cell responses to a foreign antigen,
CC namely proliferation, secretion of lymphokines, up-regulation of
CC molecules that mediate cell-cell interaction, and effective help for
CC antibody secretion by B-cells. Essential both for efficient interaction
CC between T and B-cells and for normal antibody responses to T-cell
CC dependent antigens. Does not up-regulate the production of interleukin-
CC 2, but superinduces the synthesis of interleukin-10. Prevents the
CC apoptosis of pre-activated T-cells. Plays a critical role in CD40-
CC mediated class switching of immunoglobin isotypes (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:11006126}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:11006126}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=AILIM-1;
CC IsoId=Q9R1T7-1; Sequence=Displayed;
CC Name=2; Synonyms=AILIM-2;
CC IsoId=Q9R1T7-2; Sequence=VSP_010789;
CC -!- TISSUE SPECIFICITY: Strongly expressed in the spleen and lung. Lower
CC expression seen in liver, kidney and testis.
CC {ECO:0000269|PubMed:11006126}.
CC -!- INDUCTION: Expression on T-cells is drastically induced by phorbol
CC myristate acetate (PMA) and Ca-ionophore or the engagement of CD3 and
CC CD28. {ECO:0000269|PubMed:11006126}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
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DR EMBL; AB023133; BAA82127.1; -; mRNA.
DR EMBL; AB023134; BAA82128.1; -; mRNA.
DR PIR; JC7396; JC7396.
DR PIR; JC7397; JC7397.
DR RefSeq; NP_072132.1; NM_022610.1. [Q9R1T7-1]
DR RefSeq; XP_006245099.1; XM_006245037.3. [Q9R1T7-2]
DR RefSeq; XP_006245100.1; XM_006245038.3. [Q9R1T7-1]
DR RefSeq; XP_008765358.1; XM_008767136.2. [Q9R1T7-2]
DR RefSeq; XP_017452114.1; XM_017596625.1. [Q9R1T7-2]
DR AlphaFoldDB; Q9R1T7; -.
DR SMR; Q9R1T7; -.
DR STRING; 10116.ENSRNOP00000064455; -.
DR GlyGen; Q9R1T7; 2 sites.
DR PhosphoSitePlus; Q9R1T7; -.
DR PaxDb; Q9R1T7; -.
DR Ensembl; ENSRNOT00000071226; ENSRNOP00000064455; ENSRNOG00000046196. [Q9R1T7-1]
DR GeneID; 64545; -.
DR KEGG; rno:64545; -.
DR CTD; 29851; -.
DR RGD; 620123; Icos.
DR eggNOG; ENOG502S59F; Eukaryota.
DR GeneTree; ENSGT00390000000801; -.
DR HOGENOM; CLU_1315009_0_0_1; -.
DR InParanoid; Q9R1T7; -.
DR OMA; QDKEDCF; -.
DR OrthoDB; 1298290at2759; -.
DR PhylomeDB; Q9R1T7; -.
DR Reactome; R-RNO-1257604; PIP3 activates AKT signaling.
DR Reactome; R-RNO-388841; Costimulation by the CD28 family.
DR Reactome; R-RNO-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR PRO; PR:Q9R1T7; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000046196; Expressed in thymus and 16 other tissues.
DR ExpressionAtlas; Q9R1T7; baseline and differential.
DR Genevisible; Q9R1T7; RN.
DR GO; GO:0009897; C:external side of plasma membrane; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:RGD.
DR GO; GO:0098609; P:cell-cell adhesion; IDA:RGD.
DR GO; GO:0031295; P:T cell costimulation; IDA:RGD.
DR GO; GO:0002517; P:T cell tolerance induction; IMP:RGD.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR039943; ICOS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013106; Ig_V-set.
DR PANTHER; PTHR20904; PTHR20904; 1.
DR Pfam; PF15910; V-set_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Disulfide bond;
KW Glycoprotein; Immunoglobulin domain; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000269|PubMed:11006126"
FT CHAIN 21..200
FT /note="Inducible T-cell costimulator"
FT /id="PRO_0000014808"
FT TOPO_DOM 21..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 30..133
FT /note="Ig-like V-type"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 42..109
FT /evidence="ECO:0000250"
FT DISULFID 63..83
FT /evidence="ECO:0000250"
FT VAR_SEQ 198..200
FT /note="MTS -> TAPLRALGRGEHSSCQDRN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11006126"
FT /id="VSP_010789"
SQ SEQUENCE 200 AA; 22529 MW; 0A74C35581F129D4 CRC64;
MKPYFSCVFV FCFLIKLLTG ELNDLANHRM FSFHDGGVQI SCNYPETVQQ LKMQLFKDRE
VLCDLTKTKG SGNTVSIKNP MSCPYQLSNN SVSFFLDNAD SSQGSYFLCS LSIFDPPPFQ
EKNLSGGYLL IYESQLCCQL KLWLPVGCAA FVAALLFGCI FIVWFAKKKY RSSVHDPNSE
YMFMAAVNTN KKSRLAGMTS
