ID   ICP1_ENTHI              Reviewed;         102 AA.
AC   Q6KCA4;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Amoebiasin-1;
DE   AltName: Full=Cysteine protease inhibitor 1;
DE   AltName: Full=EhICP1;
DE            Short=ICP-1;
GN   Name=AMS; Synonyms=ICP1;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=15757643; DOI=10.1016/j.febslet.2005.01.067;
RA   Riekenberg S., Witjes B., Saric M., Bruchhaus I., Scholze H.;
RT   "Identification of EhICP1, a chagasin-like cysteine protease inhibitor of
RT   Entamoeba histolytica.";
RL   FEBS Lett. 579:1573-1578(2005).
CC   -!- FUNCTION: May serve to protect the cytosol against cysteine protease
CC       molecules released by damaged intracellular vesicles. Inhibits papain
CC       and soluble trophozoite extract. {ECO:0000269|PubMed:15757643}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15757643}.
CC   -!- DOMAIN: The motif NPTTG is involved in inhibitory activity and Pro-28
CC       is the key residue.
CC   -!- MISCELLANEOUS: Present in very low amount.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I42 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ634054; CAG23944.1; -; Genomic_DNA.
DR   PDB; 6CJZ; NMR; -; A=1-102.
DR   PDBsum; 6CJZ; -.
DR   AlphaFoldDB; Q6KCA4; -.
DR   BMRB; Q6KCA4; -.
DR   SMR; Q6KCA4; -.
DR   MINT; Q6KCA4; -.
DR   STRING; 5759.rna_EHI_159600-1; -.
DR   MEROPS; I42.002; -.
DR   VEuPathDB; AmoebaDB:EHI5A_081670; -.
DR   VEuPathDB; AmoebaDB:EHI7A_046650; -.
DR   VEuPathDB; AmoebaDB:EHI8A_045830; -.
DR   VEuPathDB; AmoebaDB:EHI_159600; -.
DR   VEuPathDB; AmoebaDB:KM1_093770; -.
DR   eggNOG; ENOG502SEPD; Eukaryota.
DR   OMA; QHAPGIC; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.2020; -; 1.
DR   InterPro; IPR036331; Chagasin-like_sf.
DR   InterPro; IPR018990; Prot_inh_I42_chagasin.
DR   Pfam; PF09394; Inhibitor_I42; 1.
DR   SUPFAM; SSF141066; SSF141066; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Protease inhibitor; Thiol protease inhibitor.
FT   CHAIN           1..102
FT                   /note="Amoebiasin-1"
FT                   /id="PRO_0000218292"
FT   MOTIF           27..31
FT                   /note="NPTTG motif"
FT   HELIX           5..7
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          10..14
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          19..25
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   TURN            28..30
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          33..38
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          43..49
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          63..69
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          72..84
FT                   /evidence="ECO:0007829|PDB:6CJZ"
FT   STRAND          93..101
FT                   /evidence="ECO:0007829|PDB:6CJZ"
SQ   SEQUENCE   102 AA;  11123 MW;  D877A28C6ADFFCBD CRC64;
     MSLTEDNNNT TITIAKGENK EIILHGNPTT GYSWVVDSCE GLSNTVEYVA DQHAPGICGC
     GGKYHIKITG TQTGEGKIVL VYRRPWAPNA NDRTFTLKVN VQ