ID   ICP47_HHV2H             Reviewed;          86 AA.
AC   P14345; P89480;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   23-FEB-2022, entry version 84.
DE   RecName: Full=ICP47 protein;
DE   AltName: Full=Immediate-early protein IE12;
DE   AltName: Full=Immediate-early-5;
DE   AltName: Full=Infected cell protein 47;
DE   AltName: Full=US12 protein;
DE   AltName: Full=Vmw12;
GN   Name=US12;
OS   Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX   NCBI_TaxID=10315;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ACTIVE DOMAIN, AND INTERACTION
RP   WITH HOST TAP1 AND TAP2.
RX   PubMed=9151894; DOI=10.1084/jem.185.9.1565;
RA   Galocha B., Hill A., Barnett B.C., Dolan A., Raimondi A., Cook R.F.,
RA   Brunner J., McGeoch D.J., Ploegh H.L.;
RT   "The active site of ICP47, a herpes simplex virus-encoded inhibitor of the
RT   major histocompatibility complex (MHC)-encoded peptide transporter
RT   associated with antigen processing (TAP), maps to the NH2-terminal 35
RT   residues.";
RL   J. Exp. Med. 185:1565-1572(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9499055; DOI=10.1128/jvi.72.3.2010-2021.1998;
RA   Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.;
RT   "The genome sequence of herpes simplex virus type 2.";
RL   J. Virol. 72:2010-2021(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
RX   PubMed=6321634; DOI=10.1099/0022-1317-65-3-451;
RA   Whitton J.L., Clements J.B.;
RT   "The junctions between the repetitive and the short unique sequences of the
RT   herpes simplex virus genome are determined by the polypeptide-coding
RT   regions of two spliced immediate-early mRNAs.";
RL   J. Gen. Virol. 65:451-466(1984).
RN   [4]
RP   ACTIVE DOMAIN.
RX   PubMed=9325106; DOI=10.1006/jmbi.1997.1282;
RA   Neumann L., Kraas W., Uebel S., Jung G., Tampe R.;
RT   "The active domain of the herpes simplex virus protein ICP47: a potent
RT   inhibitor of the transporter associated with antigen processing (TAP).";
RL   J. Mol. Biol. 272:484-492(1997).
CC   -!- FUNCTION: Plays a role in the inhibition of host immune response. Binds
CC       specifically to transporters associated with antigen processing (TAP),
CC       thereby blocking peptide-binding and translocation by TAP as well as
CC       subsequent loading of peptides onto MHC class I molecules. Empty MHC I
CC       molecules are retained in the endoplasmic reticulum and ultimately
CC       directed to proteasomal degradation. In consequence, infected cells are
CC       masked for immune recognition by cytotoxic T-lymphocytes.
CC       {ECO:0000250|UniProtKB:P03170, ECO:0000269|PubMed:9151894}.
CC   -!- SUBUNIT: Interacts with host TAP1 and TAP2; these interactions inhibit
CC       the loading of peptides onto MHC class I molecules.
CC       {ECO:0000269|PubMed:9151894}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:P03170}.
CC       Host nucleus {ECO:0000250|UniProtKB:P03170}.
CC   -!- DOMAIN: The N-terminal active domain blocks peptide binding to and
CC       peptide transport by TAP. {ECO:0000269|PubMed:9151894}.
CC   -!- SIMILARITY: Belongs to the herpesviridae US12 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z86099; CAB06700.1; -; Genomic_DNA.
DR   EMBL; M29385; AAA45849.1; -; Genomic_DNA.
DR   RefSeq; YP_009137225.1; NC_001798.2.
DR   SMR; P14345; -.
DR   PRIDE; P14345; -.
DR   DNASU; 1487353; -.
DR   GeneID; 1487353; -.
DR   KEGG; vg:1487353; -.
DR   Proteomes; UP000001874; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039588; P:suppression by virus of host antigen processing and presentation; IEA:UniProtKB-KW.
DR   InterPro; IPR008026; Herpes_ICP47.
DR   Pfam; PF05363; Herpes_US12; 1.
PE   1: Evidence at protein level;
KW   Early protein; Host cytoplasm; Host nucleus; Host-virus interaction;
KW   Inhibition of host adaptive immune response by virus;
KW   Inhibition of host TAP by virus; Reference proteome; Viral immunoevasion.
FT   CHAIN           1..86
FT                   /note="ICP47 protein"
FT                   /id="PRO_0000115811"
FT   REGION          2..35
FT                   /note="Active domain"
FT                   /evidence="ECO:0000269|PubMed:9151894"
FT   REGION          33..86
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            22
FT                   /note="Binding to TAP1 subunit"
FT                   /evidence="ECO:0000269|PubMed:9151894"
FT   CONFLICT        11..12
FT                   /note="FL -> PS (in Ref. 3; AAA45849)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   86 AA;  9785 MW;  C9D6DC7C3CAA7002 CRC64;
     MSWALKTTDM FLDSSRCTHR TYGDVCAEIH KREREDREAA RTAVTDPELP LLCPPDVRSD
     PASRNPTQQT RGCARSNERQ DRVLAP