ICT1_BOVIN
ID ICT1_BOVIN Reviewed; 206 AA.
AC Q3T116; A7YWR4;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Peptidyl-tRNA hydrolase ICT1, mitochondrial;
DE EC=3.1.1.29;
DE AltName: Full=39S ribosomal protein L58, mitochondrial {ECO:0000250|UniProtKB:Q14197};
DE Short=MRP-L58;
DE AltName: Full=Immature colon carcinoma transcript 1 protein homolog;
DE Flags: Precursor;
GN Name=MRPL58 {ECO:0000250|UniProtKB:Q14197}; Synonyms=ICT1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Crossbred X Angus, and Hereford; TISSUE=Fetal brain, and Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential peptidyl-tRNA hydrolase component of the
CC mitochondrial large ribosomal subunit. Acts as a codon-independent
CC translation release factor that has lost all stop codon specificity and
CC directs the termination of translation in mitochondrion, possibly in
CC case of abortive elongation. May be involved in the hydrolysis of
CC peptidyl-tRNAs that have been prematurely terminated and thus in the
CC recycling of stalled mitochondrial ribosomes.
CC {ECO:0000250|UniProtKB:Q14197}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-
CC amino acid + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123,
CC Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191;
CC EC=3.1.1.29;
CC -!- SUBUNIT: Component of the mitochondrial ribosome large subunit (39S)
CC which comprises a 16S rRNA and about 50 distinct proteins.
CC {ECO:0000250|UniProtKB:Q14197}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q14197}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3T116-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3T116-2; Sequence=VSP_039217;
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. Mitochondrion-specific ribosomal protein mL62 subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: In contrast to other members of the family, lacks the regions
CC that come into close contact with the mRNA in the ribosomal A-site and
CC determine the STOP codon specificity, explaining the loss of codon
CC specificity for translation release factor activity. {ECO:0000305}.
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DR EMBL; BC102162; AAI02163.1; -; mRNA.
DR EMBL; BC134736; AAI34737.1; -; mRNA.
DR RefSeq; NP_001029665.1; NM_001034493.1. [Q3T116-1]
DR RefSeq; XP_005221275.1; XM_005221218.3. [Q3T116-2]
DR AlphaFoldDB; Q3T116; -.
DR SMR; Q3T116; -.
DR STRING; 9913.ENSBTAP00000004276; -.
DR PaxDb; Q3T116; -.
DR PRIDE; Q3T116; -.
DR Ensembl; ENSBTAT00000004276; ENSBTAP00000004276; ENSBTAG00000003303. [Q3T116-1]
DR GeneID; 515465; -.
DR KEGG; bta:515465; -.
DR CTD; 3396; -.
DR VEuPathDB; HostDB:ENSBTAG00000003303; -.
DR eggNOG; KOG3429; Eukaryota.
DR GeneTree; ENSGT00390000013268; -.
DR HOGENOM; CLU_089470_6_1_1; -.
DR InParanoid; Q3T116; -.
DR OMA; IIRSQEA; -.
DR OrthoDB; 1611415at2759; -.
DR TreeFam; TF315161; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000003303; Expressed in tongue muscle and 106 other tissues.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; ISS:UniProtKB.
DR GO; GO:0016150; F:translation release factor activity, codon nonspecific; ISS:UniProtKB.
DR GO; GO:0070126; P:mitochondrial translational termination; ISS:UniProtKB.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR Pfam; PF00472; RF-1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Mitochondrion; Protein biosynthesis;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT TRANSIT 1..29
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 30..206
FT /note="Peptidyl-tRNA hydrolase ICT1, mitochondrial"
FT /id="PRO_0000045759"
FT VAR_SEQ 34..62
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_039217"
SQ SEQUENCE 206 AA; 23307 MW; 1369EF2674665516 CRC64;
MAAARCLRWG LSRAEAWLLP PPTSCCHRAL HRQVEGTEFR SAYSLDKLYP ESRGADTAWR
VPGDAKQGND DIPVDRLTIS YCRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRQKMA
LTHKNKINRA GELILTSEYS RYQFRNLADC LQKIRDMIAE ASQPATEPSK EDAALQKLRI
ENMNRERLRK KRINSAIKTS RRVGTD
