ID2A_XENLA
ID ID2A_XENLA Reviewed; 133 AA.
AC Q9PWJ5; Q8AVD1; Q9YGL0;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=DNA-binding protein inhibitor ID-2-A;
DE AltName: Full=Inhibitor of DNA binding 2-A;
DE Short=XId2 {ECO:0000303|PubMed:7734394};
DE AltName: Full=Inhibitor of differentiation 2-A;
GN Name=id2-a; Synonyms=id2 {ECO:0000312|EMBL:AAH72843.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAA76634.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Regenerating limb blastema {ECO:0000312|EMBL:BAA76634.1};
RX PubMed=10646803; DOI=10.1046/j.1440-169x.1999.00477.x;
RA Shimizu-Nishikawa K., Tazawa I., Uchiyama K., Yoshizato K.;
RT "Expression of helix-loop-helix type negative regulators of differentiation
RT during limb regeneration in urodeles and anurans.";
RL Dev. Growth Differ. 41:731-743(1999).
RN [2] {ECO:0000312|EMBL:CAB38648.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Oocyte {ECO:0000269|PubMed:10525185};
RX PubMed=10525185; DOI=10.1016/s0925-4773(99)00166-5;
RA Afouda A.B., Reynaud-Deonauth S., Mohun T., Spohr G.;
RT "Localized XId3 mRNA activation in Xenopus embryos by cytoplasmic
RT polyadenylation.";
RL Mech. Dev. 88:15-31(1999).
RN [3] {ECO:0000312|EMBL:AAH72843.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH72843.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-84, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=7734394; DOI=10.1016/0925-4773(94)00319-i;
RA Wilson R., Mohun T.;
RT "XIdx, a dominant negative regulator of bHLH function in early Xenopus
RT embryos.";
RL Mech. Dev. 49:211-222(1995).
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=14651922; DOI=10.1016/j.ydbio.2003.08.017;
RA Liu K.J., Harland R.M.;
RT "Cloning and characterization of Xenopus Id4 reveals differing roles for Id
RT genes.";
RL Dev. Biol. 264:339-351(2003).
RN [6] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=15242799; DOI=10.1016/j.ydbio.2004.04.030;
RA Martinsen B.J., Frasier A.J., Baker C.V., Lohr J.L.;
RT "Cardiac neural crest ablation alters Id2 gene expression in the developing
RT heart.";
RL Dev. Biol. 272:176-190(2004).
CC -!- FUNCTION: Transcriptional regulator (lacking a basic DNA binding
CC domain) which negatively regulates the basic helix-loop-helix (bHLH)
CC transcription factors by forming heterodimers and inhibiting their DNA
CC binding and transcriptional activity. Inhibits the activity of both
CC neurogenic (neurod1/neuroD) and myogenic (myod1/myoD) bHLH factors. May
CC play a role in the regulation of the circadian clock.
CC {ECO:0000269|PubMed:14651922}.
CC -!- SUBUNIT: Heterodimer with other HLH proteins.
CC {ECO:0000250|UniProtKB:Q02363}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P41136}. Nucleus
CC {ECO:0000250|UniProtKB:P41136}.
CC -!- TISSUE SPECIFICITY: In the embryo, expressed in a range of tissues,
CC with primary expression in the developing pronephros; expressed in the
CC pronephric anlage, and by the swimming tadpole stages expressed
CC robustly in the pronephric tubules and weakly in the pronephric duct.
CC Expressed in the secondary heart field. In the developing nervous
CC system, expressed in the neural crest and in the neural folds during
CC neurula stages, and at stage 20 in the neural tube, ventral mesoderm
CC and mid-hindbrain boundary. By early tailbud stages, expressed in the
CC neural tube, somites and branchial arches. In tadpoles (stage 37/38),
CC expressed in the heart, eye, otic vesicle, somites and branchial
CC arches. Also expressed in migrating muscle cells. Expressed at a low
CC level in limbs, with expression decreasing as limbs develop, but
CC expressed at a high level in blastemas (regenerated limbs), where
CC expression is localized primarily to the blastemal epidermis. Widely
CC expressed in adults with highest expression in the spleen, skin,
CC intestine and brain, and at a much lower level in testis and heart.
CC {ECO:0000269|PubMed:10646803, ECO:0000269|PubMed:14651922,
CC ECO:0000269|PubMed:15242799, ECO:0000269|PubMed:7734394}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Maternal expression declines during cleavage and blastula stages with
CC zygotic expression increasing from gastrulation. From neurula stage
CC onwards, expression levels are relatively constant.
CC {ECO:0000269|PubMed:14651922, ECO:0000269|PubMed:7734394}.
CC -!- INDUCTION: May be induced by Notch signaling.
CC {ECO:0000269|PubMed:14651922}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH41527.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB019520; BAA76634.1; -; mRNA.
DR EMBL; AJ133647; CAB38648.1; -; mRNA.
DR EMBL; BC072843; AAH72843.1; -; mRNA.
DR EMBL; BC041527; AAH41527.1; ALT_INIT; mRNA.
DR RefSeq; NP_001081902.1; NM_001088433.1.
DR AlphaFoldDB; Q9PWJ5; -.
DR SMR; Q9PWJ5; -.
DR DNASU; 398111; -.
DR GeneID; 398111; -.
DR KEGG; xla:398111; -.
DR CTD; 398111; -.
DR Xenbase; XB-GENE-481302; id2.L.
DR OMA; SFRKNGA; -.
DR OrthoDB; 1624054at2759; -.
DR Proteomes; UP000186698; Chromosome 5L.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0090398; P:cellular senescence; ISS:UniProtKB.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISS:UniProtKB.
DR GO; GO:0061031; P:endodermal digestive tract morphogenesis; ISS:UniProtKB.
DR GO; GO:0014904; P:myotube cell development; TAS:AgBase.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0048663; P:neuron fate commitment; ISS:UniProtKB.
DR GO; GO:0045777; P:positive regulation of blood pressure; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0042752; P:regulation of circadian rhythm; ISS:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; ISS:UniProtKB.
DR GO; GO:2000177; P:regulation of neural precursor cell proliferation; ISS:UniProtKB.
DR GO; GO:0045664; P:regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR026052; DNA-bd_prot-inh.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR11723; PTHR11723; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Biological rhythms; Cytoplasm; Developmental protein; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..133
FT /note="DNA-binding protein inhibitor ID-2-A"
FT /id="PRO_0000390721"
FT DOMAIN 23..75
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT MOTIF 106..115
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250"
FT CONFLICT 104
FT /note="T -> D (in Ref. 2; CAB38648)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 133 AA; 14791 MW; 9CED81FF471D4097 CRC64;
MKAFSPVRSV RKSSLTEHSL GIARSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS
KMEILQHVID YILDLQLTLD SHPSIVSLHH LPRVGGNTSR TPLTPLNTDI SILSLQAAEF
SSEFTDESKS LCP
