ID   ID4_XENLA               Reviewed;         131 AA.
AC   Q7ZXF3; Q7ZZB5;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-binding protein inhibitor ID-4 {ECO:0000250|UniProtKB:P47928};
DE   AltName: Full=Inhibitor of DNA binding 4 {ECO:0000312|EMBL:AAR06889.1};
DE   AltName: Full=Inhibitor of differentiation 4;
GN   Name=id4 {ECO:0000312|EMBL:AAP34250.1}; Synonyms=idb4;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAP34250.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14651922; DOI=10.1016/j.ydbio.2003.08.017;
RA   Liu K.J., Harland R.M.;
RT   "Cloning and characterization of Xenopus Id4 reveals differing roles for Id
RT   genes.";
RL   Dev. Biol. 264:339-351(2003).
RN   [2] {ECO:0000312|EMBL:AAR06889.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Tribulo C., Aybar M.J.;
RT   "Cloning and expression of Xenopus inhibitor of DNA binding 4 (Id4).";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000312|EMBL:AAR06889.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tail bud {ECO:0000312|EMBL:AAH45022.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcriptional regulator (lacking a basic DNA binding
CC       domain) which negatively regulates the basic helix-loop-helix (bHLH)
CC       transcription factors by forming heterodimers and inhibiting their DNA
CC       binding and transcriptional activity. Inhibits the activity of both
CC       neurogenic (neurog1/neurogenin, neurod1/neuroD) and myogenic
CC       (myod1/myoD) bHLH factors. {ECO:0000269|PubMed:14651922}.
CC   -!- SUBUNIT: Heterodimer with other HLH proteins.
CC       {ECO:0000250|UniProtKB:P47928}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P47928,
CC       ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: During embryonic development, expressed in a number
CC       of neural tissues, including Rohon-Beard neurons, olfactory placode,
CC       eye primordia, and the trigeminal ganglia. Also expressed in other
CC       organs including the pronephros and liver primordium. Pronephric
CC       development begins by stage 25 and increases during tailbud stages.
CC       Expressed in both the tubules and the duct. As embryogenesis
CC       progresses, expressed in the migrating melanocytes and lateral line
CC       structures. {ECO:0000269|PubMed:14651922}.
CC   -!- INDUCTION: By bmp4. Inhibited by Notch-signaling.
CC       {ECO:0000269|PubMed:14651922}.
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DR   EMBL; AY279210; AAP34250.1; -; mRNA.
DR   EMBL; AY350743; AAR06889.1; -; mRNA.
DR   EMBL; BC045022; AAH45022.1; -; mRNA.
DR   RefSeq; NP_001080704.1; NM_001087235.1.
DR   RefSeq; NP_001165446.1; NM_001171975.1.
DR   RefSeq; XP_018121666.1; XM_018266177.1.
DR   AlphaFoldDB; Q7ZXF3; -.
DR   SMR; Q7ZXF3; -.
DR   DNASU; 380396; -.
DR   GeneID; 100337509; -.
DR   GeneID; 380396; -.
DR   KEGG; xla:100337509; -.
DR   KEGG; xla:380396; -.
DR   CTD; 100337509; -.
DR   CTD; 380396; -.
DR   Xenbase; XB-GENE-6464374; id4.L.
DR   Xenbase; XB-GENE-876371; id4.S.
DR   OMA; ARCKMEE; -.
DR   OrthoDB; 1624054at2759; -.
DR   Proteomes; UP000186698; Chromosome 6L.
DR   Proteomes; UP000186698; Chromosome 6S.
DR   Bgee; 100337509; Expressed in brain and 16 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0043425; F:bHLH transcription factor binding; ISS:UniProtKB.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR026052; DNA-bd_prot-inh.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   PANTHER; PTHR11723; PTHR11723; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..131
FT                   /note="DNA-binding protein inhibitor ID-4"
FT                   /id="PRO_0000390727"
FT   DOMAIN          36..88
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   CONFLICT        38
FT                   /note="Y -> F (in Ref. 1; AAP34250)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   131 AA;  14756 MW;  1964B1EB7B822D6B CRC64;
     MKAVSPVRPQ SRKAQVPSVC GELALHCLSE HSLGVARYKM EEEETLCLQY DMNDCYSRLK
     RLVPTIPPNK KVSKVEILQH VIDYILDLQL ALDTHPVLLR QQPPTRTPLT DLNTDPAALV
     VNKQGDSILC R