ID   ID5A_PROJU              Reviewed;         137 AA.
AC   P32733;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Kunitz-type trypsin inhibitor alpha chain;
OS   Prosopis juliflora (Mesquite) (Mimosa juliflora).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Caesalpinioideae; mimosoid clade;
OC   Mimoseae; Prosopis.
OX   NCBI_TaxID=13230;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=1367792; DOI=10.1016/s0031-9422(00)98207-4;
RA   Negreiros A.N., Carvalho M.M., Xavier Filho J., Blanco-Labra A.,
RA   Shewry P.R., Richardson M.;
RT   "The complete amino acid sequence of the major Kunitz trypsin inhibitor
RT   from the seeds of Prosopsis juliflora.";
RL   Phytochemistry 30:2829-2833(1991).
CC   -!- FUNCTION: Inhibition of trypsin.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain linked by a disulfide
CC       bond.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   PIR; B45588; B45588.
DR   AlphaFoldDB; P32733; -.
DR   SMR; P32733; -.
DR   MEROPS; I03.019; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..137
FT                   /note="Kunitz-type trypsin inhibitor alpha chain"
FT                   /id="PRO_0000083300"
FT   SITE            64..65
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        40..86
FT                   /evidence="ECO:0000250"
FT   DISULFID        131
FT                   /note="Interchain (with beta chain)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   137 AA;  15469 MW;  6AE2B3C354C1D0E5 CRC64;
     QELLDVDGEI LRNGGSYYIL PAFRGKGGGL ELAKTEGETC PLTVVQARSE TDRGLPASIW
     SPPRIAIIRP GFSLNIEFRP RNPSACHRES SLQWKVEEES QQVKIAVKED ARGFGPFRIR
     PHRDDYKLVY CDEGQKR