IDA_ARATH
ID IDA_ARATH Reviewed; 77 AA.
AC Q8LAD7;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Protein IDA {ECO:0000303|PubMed:12972671};
DE AltName: Full=Protein INFLORESCENCE DEFICIENT IN ABSCISSION {ECO:0000303|PubMed:12972671};
DE Flags: Precursor;
GN Name=IDA {ECO:0000303|PubMed:12972671};
GN OrderedLocusNames=At1g68765 {ECO:0000312|Araport:AT1G68765};
GN ORFNames=F14K14 {ECO:0000312|EMBL:AC011914};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=12972671; DOI=10.1105/tpc.014365;
RA Butenko M.A., Patterson S.E., Grini P.E., Stenvik G.-E., Amundsen S.S.,
RA Mandal A., Aalen R.B.;
RT "Inflorescence deficient in abscission controls floral organ abscission in
RT Arabidopsis and identifies a novel family of putative ligands in plants.";
RL Plant Cell 15:2296-2307(2003).
RN [6]
RP INDUCTION BY WOUNDING.
RX PubMed=16990374; DOI=10.1093/jxb/erl130;
RA Butenko M.A., Stenvik G.-E., Alm V., Saether B., Patterson S.E.,
RA Aalen R.B.;
RT "Ethylene-dependent and -independent pathways controlling floral abscission
RT are revealed to converge using promoter::reporter gene constructs in the
RT ida abscission mutant.";
RL J. Exp. Bot. 57:3627-3637(2006).
RN [7]
RP FUNCTION.
RX PubMed=18660431; DOI=10.1105/tpc.108.059139;
RA Stenvik G.-E., Tandstad N.M., Guo Y., Shi C.-L., Kristiansen W.,
RA Holmgren A., Clark S.E., Aalen R.B., Butenko M.A.;
RT "The EPIP peptide of INFLORESCENCE DEFICIENT IN ABSCISSION is sufficient to
RT induce abscission in arabidopsis through the receptor-like kinases HAESA
RT and HAESA-LIKE2.";
RL Plant Cell 20:1805-1817(2008).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. C24, cv. Columbia, and cv. Landsberg erecta;
RX PubMed=23963677; DOI=10.1093/jxb/ert232;
RA Liu B., Butenko M.A., Shi C.-L., Bolivar J.L., Winge P., Stenvik G.-E.,
RA Vie A.K., Leslie M.E., Brembu T., Kristiansen W., Bones A.M.,
RA Patterson S.E., Liljegren S.J., Aalen R.B.;
RT "NEVERSHED and INFLORESCENCE DEFICIENT IN ABSCISSION are differentially
RT required for cell expansion and cell separation during floral organ
RT abscission in Arabidopsis thaliana.";
RL J. Exp. Bot. 64:5345-5357(2013).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 58-69 IN COMPLEX WITH RLK5,
RP FUNCTION, INTERACTION WITH RLK5, AND MUTAGENESIS OF LYS-66 AND
RP 66-LYS-ARG-67.
RX PubMed=27058169; DOI=10.7554/elife.15075;
RA Santiago J., Brandt B., Wildhagen M., Hohmann U., Hothorn L.A.,
RA Butenko M.A., Hothorn M.;
RT "Mechanistic insight into a peptide hormone signaling complex mediating
RT floral organ abscission.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Involved in an ethylene-independent separation step of floral
CC abscission (PubMed:18660431, PubMed:23963677, PubMed:27058169).
CC Promotes abscission zone (AZ) cells rounding (PubMed:23963677,
CC PubMed:27058169). May act with RLK5 and HSL2 as ligand-receptor pairs
CC (PubMed:18660431). {ECO:0000269|PubMed:18660431,
CC ECO:0000269|PubMed:23963677, ECO:0000269|PubMed:27058169}.
CC -!- SUBUNIT: Interaction with RLK5. {ECO:0000269|PubMed:27058169}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000269|PubMed:12972671}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in the floral abscission
CC zone. {ECO:0000269|PubMed:12972671}.
CC -!- DEVELOPMENTAL STAGE: Expressed only during floral abscission.
CC {ECO:0000269|PubMed:12972671}.
CC -!- INDUCTION: By wounding. {ECO:0000269|PubMed:16990374}.
CC -!- DOMAIN: An extended PIP motif (50-69) is sufficient for abscission
CC induction.
CC -!- DISRUPTION PHENOTYPE: Floral organs remain attached to the plant body
CC after the shedding of mature seeds despite a normal floral abscission
CC zone development, including abnormal petal breakstrength (pBS)
CC (PubMed:12972671, PubMed:23963677). Delayed cell-wall loosening during
CC shedding associated with less pronounced and delayed abscission zone
CC (AZ) cells rounding (PubMed:23963677). {ECO:0000269|PubMed:12972671,
CC ECO:0000269|PubMed:23963677}.
CC -!- MISCELLANEOUS: IDL1 is capable of replacing the activity of IDA while
CC IDL2, IDL3, IDL4 and IDL5 are only partially redundant.
CC -!- MISCELLANEOUS: IDA is processed in vitro by the same proteolytic
CC activity releasing the CLE peptide from CLV3.
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DR EMBL; AC011914; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE34837.1; -; Genomic_DNA.
DR EMBL; AY087883; AAM65435.1; -; mRNA.
DR EMBL; BT025310; ABF47126.1; -; mRNA.
DR RefSeq; NP_564941.1; NM_105550.2.
DR PDB; 5IXQ; X-ray; 1.86 A; B=58-69.
DR PDB; 5IXT; X-ray; 1.94 A; B=54-69.
DR PDB; 5IYX; X-ray; 2.43 A; B=56-69.
DR PDB; 7ODV; X-ray; 2.31 A; CCC/FFF=57-69.
DR PDBsum; 5IXQ; -.
DR PDBsum; 5IXT; -.
DR PDBsum; 5IYX; -.
DR PDBsum; 7ODV; -.
DR AlphaFoldDB; Q8LAD7; -.
DR SMR; Q8LAD7; -.
DR STRING; 3702.AT1G68765.1; -.
DR PaxDb; Q8LAD7; -.
DR PRIDE; Q8LAD7; -.
DR EnsemblPlants; AT1G68765.1; AT1G68765.1; AT1G68765.
DR GeneID; 843208; -.
DR Gramene; AT1G68765.1; AT1G68765.1; AT1G68765.
DR KEGG; ath:AT1G68765; -.
DR Araport; AT1G68765; -.
DR TAIR; locus:505006209; AT1G68765.
DR eggNOG; ENOG502S9CH; Eukaryota.
DR HOGENOM; CLU_180384_0_0_1; -.
DR OMA; PCRTMMV; -.
DR OrthoDB; 1644639at2759; -.
DR PhylomeDB; Q8LAD7; -.
DR PRO; PR:Q8LAD7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LAD7; baseline and differential.
DR Genevisible; Q8LAD7; AT.
DR GO; GO:0048046; C:apoplast; TAS:TAIR.
DR GO; GO:0005576; C:extracellular region; IDA:TAIR.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0005102; F:signaling receptor binding; TAS:TAIR.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:TAIR.
DR GO; GO:0010227; P:floral organ abscission; IMP:UniProtKB.
DR GO; GO:0010102; P:lateral root morphogenesis; IMP:TAIR.
DR GO; GO:0060866; P:leaf abscission; IMP:TAIR.
DR GO; GO:0045490; P:pectin catabolic process; IMP:TAIR.
DR GO; GO:0060305; P:regulation of cell diameter; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IGI:TAIR.
DR GO; GO:0009723; P:response to ethylene; IMP:TAIR.
DR InterPro; IPR039639; IDA-like.
DR PANTHER; PTHR33599; PTHR33599; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..77
FT /note="Protein IDA"
FT /id="PRO_0000383588"
FT REGION 56..69
FT /note="RLK5-binding"
FT /evidence="ECO:0000269|PubMed:27058169,
FT ECO:0007744|PDB:5IXQ, ECO:0007744|PDB:5IXT,
FT ECO:0007744|PDB:5IYX"
FT MUTAGEN 66..67
FT /note="KR->AA: Reduced activity leading to delayed floral
FT abscission."
FT /evidence="ECO:0000269|PubMed:27058169"
FT MUTAGEN 66
FT /note="K->A: Reduced binding affinity for RLK5."
FT /evidence="ECO:0000269|PubMed:27058169"
SQ SEQUENCE 77 AA; 8433 MW; 73F14D0FBA295CC1 CRC64;
MAPCRTMMVL LCFVLFLAAS SSCVAAARIG ATMEMKKNIK RLTFKNSHIF GYLPKGVPIP
PSAPSKRHNS FVNSLPH
