ID   IDE3_ERYLA              Reviewed;         172 AA.
AC   P68171; P07475;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Trypsin inhibitor DE-3;
OS   Erythrina latissima (Broad-leaved coral tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Erythrina.
OX   NCBI_TaxID=3844;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=3902809; DOI=10.1016/s0021-9258(17)38817-8;
RA   Joubert F.J., Heussen C., Dowdle E.B.D.;
RT   "The complete amino acid sequence of trypsin inhibitor DE-3 from Erythrina
RT   latissima seeds.";
RL   J. Biol. Chem. 260:12948-12953(1985).
CC   -!- FUNCTION: Inhibition of trypsin.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   PIR; A24082; A24082.
DR   AlphaFoldDB; P68171; -.
DR   SMR; P68171; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..172
FT                   /note="Trypsin inhibitor DE-3"
FT                   /id="PRO_0000083288"
FT   SITE            63..64
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        39..83
FT                   /evidence="ECO:0000250"
FT   DISULFID        132..139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   172 AA;  19258 MW;  2F71CED15C247780 CRC64;
     VLLDGNGEVV QNGGTYYLLP QVWAQGGGVQ LAKTGEETCP LTVVQSPNEL SDGKPIRIES
     RLRSTFIPDD DEVRIGFAYA PKCAPSPWWT VVEDEQEGLS VKLSEDESTQ FDYPFKFEQV
     SDKLHSYKLL YCEGKHEKCA SIGINRDQKG YRRLVVTEDN PLTVVLKKDE SS