ID   IDE3_ERYVA              Reviewed;         172 AA.
AC   P81366;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Trypsin inhibitor DE-3;
DE   AltName: Full=ETIA;
OS   Erythrina variegata (Indian coral tree) (Erythrina indica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Erythrina.
OX   NCBI_TaxID=3845;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=Var. Orientalis; TISSUE=Seed;
RX   PubMed=1369077; DOI=10.1271/bbb.56.1819;
RA   Kouzuma Y., Suetake M., Kimura M., Yamasaki N.;
RT   "Isolation and primary structure of proteinase inhibitors from Erythrina
RT   variegata (Linn.) var. Orientalis seeds.";
RL   Biosci. Biotechnol. Biochem. 56:1819-1824(1992).
CC   -!- FUNCTION: Inhibition of trypsin.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   PIR; JH0780; JH0780.
DR   AlphaFoldDB; P81366; -.
DR   SMR; P81366; -.
DR   MEROPS; I03.011; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..172
FT                   /note="Trypsin inhibitor DE-3"
FT                   /id="PRO_0000083289"
FT   SITE            63..64
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        39..83
FT                   /evidence="ECO:0000250"
FT   DISULFID        132..139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   172 AA;  19240 MW;  ED9AB4D68F343522 CRC64;
     VLLDGNGEVV QNGGTYYLLP QVWAQGGGVQ LAKTGEETCP LTVVQSPNEL SNGKPIRIES
     RLRSAFIPDD DKVRIGFAYA PKCAPSPWWT VLEDEQEGLS VKLSEDESTQ FDYPFKFEQV
     SDKLHSYKLL YCEGKHEKCA SIGINRDQKG YRRLVVTEDN PLTVVLKKDE SS