ID   IDGFL_BOMMO             Reviewed;         433 AA.
AC   Q9GV28; P82223; P82224;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Chitinase-like protein EN03;
DE   AltName: Full=Imaginal disk growth factor-like protein;
DE   Flags: Precursor;
GN   Name=EN03 {ECO:0000312|EMBL:BAB16695.1};
OS   Bombyx mori (Silk moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Bombycidae; Bombycinae; Bombyx.
OX   NCBI_TaxID=7091;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAB16695.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   STRAIN=Kinshu X Showa {ECO:0000269|PubMed:11222941};
RC   TISSUE=Anterior silk gland {ECO:0000269|PubMed:11222941};
RX   PubMed=11222941; DOI=10.1016/s0965-1748(00)00124-7;
RA   Tsuzuki S., Iwami M., Sakurai S.;
RT   "Ecdysteroid-inducible genes in the programmed cell death during insect
RT   metamorphosis.";
RL   Insect Biochem. Mol. Biol. 31:321-331(2001).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 17-27.
RC   STRAIN=Xinhang X Keming {ECO:0000269|PubMed:11280994};
RC   TISSUE=Body wall {ECO:0000269|PubMed:11280994}, and
RC   Fat body {ECO:0000269|PubMed:11280994};
RX   PubMed=11280994;
RA   Zhong B.-X.;
RT   "Protein database for several tissues derived from five instar of
RT   silkworm.";
RL   Yi Chuan Xue Bao 28:217-224(2001).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- INDUCTION: Induced 2 to 4 hours after treatment with 20-hydroxyecdysone
CC       (20E). Repressed by 8 hours after treatment with 20E, except in the
CC       presence of cycloheximide. {ECO:0000269|PubMed:11222941}.
CC   -!- MISCELLANEOUS: Lacks the typical Glu active site in position 152 that
CC       is replaced by a Gln residue, preventing the hydrolase activity. Its
CC       precise function remains unclear. {ECO:0000269|PubMed:11222941}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. IDGF
CC       subfamily. {ECO:0000255}.
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DR   EMBL; AB041634; BAB16695.1; -; mRNA.
DR   RefSeq; NP_001036847.1; NM_001043382.1.
DR   STRING; 7091.BGIBMGA000648-TA; -.
DR   PRIDE; Q9GV28; -.
DR   GeneID; 692387; -.
DR   KEGG; bmor:692387; -.
DR   CTD; 100160032; -.
DR   eggNOG; KOG2806; Eukaryota.
DR   HOGENOM; CLU_002833_3_2_1; -.
DR   InParanoid; Q9GV28; -.
DR   OrthoDB; 482694at2759; -.
DR   BRENDA; 2.4.1.16; 890.
DR   Proteomes; UP000005204; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008061; F:chitin binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd02873; GH18_IDGF; 1.
DR   Gene3D; 3.10.50.10; -; 1.
DR   InterPro; IPR011583; Chitinase_II.
DR   InterPro; IPR029070; Chitinase_insertion_sf.
DR   InterPro; IPR001223; Glyco_hydro18_cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR015520; IDGF.
DR   PANTHER; PTHR11177:SF235; PTHR11177:SF235; 1.
DR   Pfam; PF00704; Glyco_hydro_18; 1.
DR   SMART; SM00636; Glyco_18; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF54556; SSF54556; 1.
DR   PROSITE; PS51910; GH18_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000269|PubMed:11280994"
FT   CHAIN           17..433
FT                   /note="Chitinase-like protein EN03"
FT                   /evidence="ECO:0000269|PubMed:11280994"
FT                   /id="PRO_0000274553"
FT   DOMAIN          23..433
FT                   /note="GH18"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   CARBOHYD        220
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        27..54
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   DISULFID        337..418
FT                   /evidence="ECO:0000250|UniProtKB:Q9V3D4"
SQ   SEQUENCE   433 AA;  48144 MW;  EB1406C67328A00B CRC64;
     MKLFIALVGL LALAKALPAV THSKVLCYYD SRSYVRESQA RMLPLDLDPA LSFCTHLLYG
     YAVIQPDTYK LVSLNENLDI DRTHDNYRAI TSLKAKYPGL TVLLSVGGDA DTEEPEKYNL
     LLESQQARTA FINSGVLLAE QYGFDGIDLA WQFPRVKPKK IRSTWGSLWH GIKKTFGTTP
     VDEKESEHRE GFTALVRELK QALIHKPKMQ LGVTVLPNVN STIYHDVPAI INLVDYVNVG
     AYDYYTPTRN NKEADYTAPI YTPQNRNPLQ NADAAVTYWL TSGAPSQKIV LSXRLRSYLE
     TGXDSEIAGV PPIHTDGPGE AGPYVKTEGL LSYPEVCGKL INPNQQKGMR PHLRKVTDPS
     KRFGTYAFRL PDDNGEGGIW VSYEDPDTAG QKAAYVKSKN LGGVAIVDLS LDDFRGLCTG
     DKYPILRAAK YRL