APC15_MOUSE
ID APC15_MOUSE Reviewed; 132 AA.
AC P60007; Q3UT90; Q3UXV5; Q9CXK2; Q9Y269;
DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Anaphase-promoting complex subunit 15;
DE Short=APC15;
GN Name=Anapc15;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Egg, Embryonic head, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls
CC progression through mitosis and the G1 phase of the cell cycle. In the
CC complex, plays a role in the release of the mitotic checkpoint complex
CC (MCC) from the APC/C: not required for APC/C activity itself, but
CC promotes the turnover of CDC20 and MCC on the APC/C, thereby
CC participating in the responsiveness of the spindle assembly checkpoint.
CC Also required for degradation of CDC20 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The mammalian APC/C is composed at least of 14 distinct
CC subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6,
CC ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and
CC ANAPC16 that assemble into a complex of at least 19 chains with a
CC combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1
CC and FBXO5. {ECO:0000250|UniProtKB:P60006}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P60007-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P60007-2; Sequence=VSP_043771;
CC -!- SIMILARITY: Belongs to the APC15 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-12 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AK014309; BAB29261.2; -; mRNA.
DR EMBL; AK135201; BAE22458.1; -; mRNA.
DR EMBL; AK139635; BAE24090.1; -; mRNA.
DR EMBL; AC167240; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC006736; AAH06736.2; -; mRNA.
DR CCDS; CCDS21518.1; -. [P60007-1]
DR CCDS; CCDS80760.1; -. [P60007-2]
DR RefSeq; NP_001278277.1; NM_001291348.1. [P60007-2]
DR RefSeq; NP_001278278.1; NM_001291349.1.
DR RefSeq; NP_001278281.1; NM_001291352.1. [P60007-1]
DR RefSeq; NP_001278282.1; NM_001291353.1. [P60007-1]
DR RefSeq; NP_081808.2; NM_027532.4. [P60007-1]
DR RefSeq; XP_017167848.1; XM_017312359.1.
DR AlphaFoldDB; P60007; -.
DR SMR; P60007; -.
DR MaxQB; P60007; -.
DR PaxDb; P60007; -.
DR PRIDE; P60007; -.
DR ProteomicsDB; 296266; -. [P60007-1]
DR ProteomicsDB; 296267; -. [P60007-2]
DR Antibodypedia; 44861; 36 antibodies from 16 providers.
DR DNASU; 75430; -.
DR Ensembl; ENSMUST00000035395; ENSMUSP00000040286; ENSMUSG00000030649. [P60007-2]
DR Ensembl; ENSMUST00000106978; ENSMUSP00000102591; ENSMUSG00000030649. [P60007-1]
DR Ensembl; ENSMUST00000144207; ENSMUSP00000114771; ENSMUSG00000030649. [P60007-1]
DR Ensembl; ENSMUST00000178851; ENSMUSP00000136164; ENSMUSG00000030649. [P60007-1]
DR GeneID; 75430; -.
DR KEGG; mmu:75430; -.
DR UCSC; uc009ipp.2; mouse. [P60007-1]
DR UCSC; uc009ips.2; mouse. [P60007-2]
DR CTD; 25906; -.
DR MGI; MGI:1922680; Anapc15.
DR VEuPathDB; HostDB:ENSMUSG00000030649; -.
DR eggNOG; ENOG502RZUK; Eukaryota.
DR GeneTree; ENSGT00390000000938; -.
DR InParanoid; P60007; -.
DR Reactome; R-MMU-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR Reactome; R-MMU-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR Reactome; R-MMU-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR Reactome; R-MMU-174154; APC/C:Cdc20 mediated degradation of Securin.
DR Reactome; R-MMU-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-MMU-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR Reactome; R-MMU-176407; Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
DR Reactome; R-MMU-176408; Regulation of APC/C activators between G1/S and early anaphase.
DR Reactome; R-MMU-176409; APC/C:Cdc20 mediated degradation of mitotic proteins.
DR Reactome; R-MMU-176412; Phosphorylation of the APC/C.
DR Reactome; R-MMU-179409; APC-Cdc20 mediated degradation of Nek2A.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR Reactome; R-MMU-68867; Assembly of the pre-replicative complex.
DR Reactome; R-MMU-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR BioGRID-ORCS; 75430; 15 hits in 57 CRISPR screens.
DR ChiTaRS; Anapc15; mouse.
DR PRO; PR:P60007; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P60007; protein.
DR Bgee; ENSMUSG00000030649; Expressed in morula and 253 other tissues.
DR ExpressionAtlas; P60007; baseline and differential.
DR Genevisible; P60007; MM.
DR GO; GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0090266; P:regulation of mitotic cell cycle spindle assembly checkpoint; ISS:UniProtKB.
DR InterPro; IPR026182; ANAPC15.
DR PANTHER; PTHR22526; PTHR22526; 1.
DR Pfam; PF15243; ANAPC15; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Cell division; Mitosis;
KW Reference proteome.
FT CHAIN 1..132
FT /note="Anaphase-promoting complex subunit 15"
FT /id="PRO_0000084073"
FT REGION 58..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..124
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..10
FT /note="MEPGLKLSLG -> MRKLRPREVKCIGQNQRAR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_043771"
SQ SEQUENCE 132 AA; 15378 MW; 211303F56397D993 CRC64;
MEPGLKLSLG AMSTLFPSLF PRVTETLWFN LDRPCVEETE LQQQEQQHQA WLQSIAEKDN
NLVPIGKPAS EHYDDEEEED DEDDEDSEED SEDDEDMQDM DEMNDYNESP DDGEVNEVDM
EGNEQDQDQW MI
