ID   IDHC_NAEFO              Reviewed;          20 AA.
AC   P83726;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 1.
DT   07-OCT-2020, entry version 51.
DE   RecName: Full=Isocitrate dehydrogenase [NADP];
DE            Short=IDH;
DE            EC=1.1.1.42;
DE   AltName: Full=IDP;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=NF033;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   Flags: Fragment;
OS   Naegleria fowleri (Brain eating amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5763 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 30214 / Nf 66;
RA   Omura M., Furushima-Shimogawara R., Izumiyama S., Endo T.;
RT   "Comparative study of protein profiles on pathogenic and nonpathogenic
RT   Naegleria species by 2D-PAGE.";
RL   Submitted (DEC-2003) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC         Evidence={ECO:0000250|UniProtKB:Q06197};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 6.8,
CC       its MW is: 32.3 kDa. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000305}.
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DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Magnesium; Manganese; NADP;
KW   Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN           1..>20
FT                   /note="Isocitrate dehydrogenase [NADP]"
FT                   /id="PRO_0000083582"
FT   NON_TER         20
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   20 AA;  2215 MW;  4A8244E9BA01D677 CRC64;
     AXFQKIKVAN PVVELDGDEM