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IDHP_HUMAN
ID   IDHP_HUMAN              Reviewed;         452 AA.
AC   P48735; B2R6L6; B4DFL2; Q96GT3;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2002, sequence version 2.
DT   03-AUG-2022, entry version 210.
DE   RecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial;
DE            Short=IDH;
DE            EC=1.1.1.42 {ECO:0000269|PubMed:22416140, ECO:0000305|PubMed:19228619};
DE   AltName: Full=ICD-M;
DE   AltName: Full=IDP;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   Flags: Precursor;
GN   Name=IDH2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Heart;
RA   Huh T.-L., Oh I.-U., Kim Y.O., Huh J.-W., Song B.J.;
RL   Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain cortex, Heart, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16572171; DOI=10.1038/nature04601;
RA   Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA   Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA   FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA   Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA   Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA   DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA   Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA   Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA   Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA   O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA   Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA   Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT   "Analysis of the DNA sequence and duplication history of human chromosome
RT   15.";
RL   Nature 440:671-675(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Colon, and Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67; LYS-106; LYS-155; LYS-166;
RP   LYS-180; LYS-256; LYS-263; LYS-272; LYS-275; LYS-282 AND LYS-442, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19608861; DOI=10.1126/science.1175371;
RA   Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA   Olsen J.V., Mann M.;
RT   "Lysine acetylation targets protein complexes and co-regulates major
RT   cellular functions.";
RL   Science 325:834-840(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [8]
RP   FUNCTION, CATALYTIC ACTIVITY, ACETYLATION AT LYS-413, AND MUTAGENESIS OF
RP   LYS-413.
RX   PubMed=22416140; DOI=10.1074/jbc.m112.355206;
RA   Yu W., Dittenhafer-Reed K.E., Denu J.M.;
RT   "SIRT3 protein deacetylates isocitrate dehydrogenase 2 (IDH2) and regulates
RT   mitochondrial redox status.";
RL   J. Biol. Chem. 287:14078-14086(2012).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
RN   [11]
RP   VARIANTS GLM GLY-172; LYS-172 AND MET-172, FUNCTION, CATALYTIC ACTIVITY,
RP   AND CHARACTERIZATION OF VARIANTS GLM GLY-172; LYS-172 AND MET-172.
RX   PubMed=19228619; DOI=10.1056/nejmoa0808710;
RA   Yan H., Parsons D.W., Jin G., McLendon R., Rasheed B.A., Yuan W., Kos I.,
RA   Batinic-Haberle I., Jones S., Riggins G.J., Friedman H., Friedman A.,
RA   Reardon D., Herndon J., Kinzler K.W., Velculescu V.E., Vogelstein B.,
RA   Bigner D.D.;
RT   "IDH1 and IDH2 mutations in gliomas.";
RL   N. Engl. J. Med. 360:765-773(2009).
RN   [12]
RP   VARIANTS D2HGA2 GLN-140 AND GLY-140.
RX   PubMed=20847235; DOI=10.1126/science.1192632;
RA   Kranendijk M., Struys E.A., van Schaftingen E., Gibson K.M., Kanhai W.A.,
RA   van der Knaap M.S., Amiel J., Buist N.R., Das A.M., de Klerk J.B.,
RA   Feigenbaum A.S., Grange D.K., Hofstede F.C., Holme E., Kirk E.P.,
RA   Korman S.H., Morava E., Morris A., Smeitink J., Sukhai R.N., Vallance H.,
RA   Jakobs C., Salomons G.S.;
RT   "IDH2 mutations in patients with D-2-hydroxyglutaric aciduria.";
RL   Science 330:336-336(2010).
RN   [13]
RP   VARIANTS GLM LEU-158; SER-162 AND LYS-172.
RX   PubMed=25495392; DOI=10.1111/neup.12187;
RA   Koh J., Cho H., Kim H., Kim S.I., Yun S., Park C.K., Lee S.H., Choi S.H.,
RA   Park S.H.;
RT   "IDH2 mutation in gliomas including novel mutation.";
RL   Neuropathology 35:236-244(2015).
RN   [14]
RP   VARIANTS SER-172; THR-172 AND TRP-172, AND INVOLVEMENT IN DISEASE.
RX   PubMed=26161668; DOI=10.1371/journal.pone.0131998;
RA   Jin Y., Elalaf H., Watanabe M., Tamaki S., Hineno S., Matsunaga K.,
RA   Woltjen K., Kobayashi Y., Nagata S., Ikeya M., Kato T. Jr., Okamoto T.,
RA   Matsuda S., Toguchida J.;
RT   "Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in
RT   Association with Gene-Specific Histone Modifications to Cartilage- and
RT   Bone-Related Genes.";
RL   PLoS ONE 10:E0131998-E0131998(2015).
CC   -!- FUNCTION: Plays a role in intermediary metabolism and energy production
CC       (PubMed:22416140, PubMed:19228619). It may tightly associate or
CC       interact with the pyruvate dehydrogenase complex (PubMed:22416140,
CC       PubMed:19228619). {ECO:0000269|PubMed:19228619,
CC       ECO:0000269|PubMed:22416140}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC         Evidence={ECO:0000269|PubMed:22416140, ECO:0000305|PubMed:19228619};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:P33198};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000250|UniProtKB:P33198};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000250|UniProtKB:P33198};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P33198}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:P33198}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P48735-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P48735-2; Sequence=VSP_056278;
CC   -!- PTM: Acetylation at Lys-413 dramatically reduces catalytic activity.
CC       Deacetylated by SIRT3. {ECO:0000269|PubMed:22416140}.
CC   -!- DISEASE: D-2-hydroxyglutaric aciduria 2 (D2HGA2) [MIM:613657]: A
CC       neurometabolic disorder causing developmental delay, epilepsy,
CC       hypotonia, and dysmorphic features. Both a mild and a severe phenotype
CC       exist. The severe phenotype is homogeneous and is characterized by
CC       early infantile-onset epileptic encephalopathy and cardiomyopathy. The
CC       mild phenotype has a more variable clinical presentation. Diagnosis is
CC       based on the presence of an excess of D-2-hydroxyglutaric acid in the
CC       urine. {ECO:0000269|PubMed:20847235}. Note=The disease is caused by
CC       variants affecting the gene represented in this entry.
CC   -!- DISEASE: Glioma (GLM) [MIM:137800]: Gliomas are benign or malignant
CC       central nervous system neoplasms derived from glial cells. They
CC       comprise astrocytomas and glioblastoma multiforme that are derived from
CC       astrocytes, oligodendrogliomas derived from oligodendrocytes and
CC       ependymomas derived from ependymocytes. {ECO:0000269|PubMed:19228619,
CC       ECO:0000269|PubMed:25495392}. Note=The gene represented in this entry
CC       is involved in disease pathogenesis.
CC   -!- DISEASE: Note=enetic variations are associated with cartilaginous
CC       tumors such as enchondroma or chondrosarcoma.
CC       {ECO:0000269|PubMed:26161668}.
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; X69433; CAA49208.1; -; mRNA.
DR   EMBL; AK294148; BAG57473.1; -; mRNA.
DR   EMBL; AK312627; BAG35513.1; -; mRNA.
DR   EMBL; AK316388; BAH14759.1; -; mRNA.
DR   EMBL; AC087284; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC092769; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471101; EAX02082.1; -; Genomic_DNA.
DR   EMBL; BC009244; AAH09244.1; -; mRNA.
DR   EMBL; BC071828; AAH71828.1; -; mRNA.
DR   CCDS; CCDS10359.1; -. [P48735-1]
DR   CCDS; CCDS76792.1; -. [P48735-2]
DR   PIR; S57499; S57499.
DR   RefSeq; NP_001276839.1; NM_001289910.1. [P48735-2]
DR   RefSeq; NP_001277043.1; NM_001290114.1.
DR   RefSeq; NP_002159.2; NM_002168.3. [P48735-1]
DR   PDB; 4JA8; X-ray; 1.55 A; A/B=41-452.
DR   PDB; 5GIS; X-ray; 1.93 A; C=165-180.
DR   PDB; 5I95; X-ray; 1.54 A; A=40-452.
DR   PDB; 5I96; X-ray; 1.55 A; A/B=1-452.
DR   PDB; 5SVN; X-ray; 2.10 A; A/B=40-452.
DR   PDB; 5SVO; X-ray; 1.87 A; A/B=40-452.
DR   PDB; 6ADI; X-ray; 1.97 A; A/B=41-452.
DR   PDB; 6UJ7; X-ray; 1.90 A; C/F=134-143.
DR   PDB; 6UJ8; X-ray; 2.25 A; C/F=134-143.
DR   PDB; 6UJ9; X-ray; 2.90 A; C=134-143.
DR   PDB; 6VFZ; X-ray; 1.99 A; A/B=1-452.
DR   PDBsum; 4JA8; -.
DR   PDBsum; 5GIS; -.
DR   PDBsum; 5I95; -.
DR   PDBsum; 5I96; -.
DR   PDBsum; 5SVN; -.
DR   PDBsum; 5SVO; -.
DR   PDBsum; 6ADI; -.
DR   PDBsum; 6UJ7; -.
DR   PDBsum; 6UJ8; -.
DR   PDBsum; 6UJ9; -.
DR   PDBsum; 6VFZ; -.
DR   AlphaFoldDB; P48735; -.
DR   SMR; P48735; -.
DR   BioGRID; 109644; 233.
DR   CORUM; P48735; -.
DR   DIP; DIP-61416N; -.
DR   IntAct; P48735; 25.
DR   MINT; P48735; -.
DR   STRING; 9606.ENSP00000331897; -.
DR   BindingDB; P48735; -.
DR   ChEMBL; CHEMBL3991501; -.
DR   DrugBank; DB13874; Enasidenib.
DR   DrugBank; DB01727; Isocitric Acid.
DR   DrugCentral; P48735; -.
DR   CarbonylDB; P48735; -.
DR   GlyGen; P48735; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; P48735; -.
DR   MetOSite; P48735; -.
DR   PhosphoSitePlus; P48735; -.
DR   SwissPalm; P48735; -.
DR   BioMuta; IDH2; -.
DR   DMDM; 20141568; -.
DR   OGP; P48735; -.
DR   UCD-2DPAGE; P48735; -.
DR   CPTAC; CPTAC-221; -.
DR   CPTAC; CPTAC-222; -.
DR   EPD; P48735; -.
DR   jPOST; P48735; -.
DR   MassIVE; P48735; -.
DR   MaxQB; P48735; -.
DR   PaxDb; P48735; -.
DR   PeptideAtlas; P48735; -.
DR   PRIDE; P48735; -.
DR   ProteomicsDB; 4054; -.
DR   ProteomicsDB; 55932; -. [P48735-1]
DR   ABCD; P48735; 1 sequenced antibody.
DR   Antibodypedia; 15858; 476 antibodies from 44 providers.
DR   DNASU; 3418; -.
DR   Ensembl; ENST00000330062.8; ENSP00000331897.4; ENSG00000182054.10. [P48735-1]
DR   Ensembl; ENST00000540499.2; ENSP00000446147.2; ENSG00000182054.10. [P48735-2]
DR   GeneID; 3418; -.
DR   KEGG; hsa:3418; -.
DR   MANE-Select; ENST00000330062.8; ENSP00000331897.4; NM_002168.4; NP_002159.2.
DR   UCSC; uc002box.4; human. [P48735-1]
DR   CTD; 3418; -.
DR   DisGeNET; 3418; -.
DR   GeneCards; IDH2; -.
DR   HGNC; HGNC:5383; IDH2.
DR   HPA; ENSG00000182054; Group enriched (skeletal muscle, tongue).
DR   MalaCards; IDH2; -.
DR   MIM; 137800; phenotype.
DR   MIM; 147650; gene.
DR   MIM; 613657; phenotype.
DR   neXtProt; NX_P48735; -.
DR   OpenTargets; ENSG00000182054; -.
DR   Orphanet; 251589; Anaplastic astrocytoma.
DR   Orphanet; 251663; Anaplastic oligoastrocytoma.
DR   Orphanet; 251630; Anaplastic oligodendroglioma.
DR   Orphanet; 79315; D-2-hydroxyglutaric aciduria.
DR   Orphanet; 251601; Fibrillary astrocytoma.
DR   Orphanet; 251604; Gemistocytic astrocytoma.
DR   Orphanet; 163634; Maffucci syndrome.
DR   Orphanet; 251656; Oligoastrocytoma.
DR   Orphanet; 251627; Oligodendroglioma.
DR   Orphanet; 296; Ollier disease.
DR   Orphanet; 251598; Protoplasmic astrocytoma.
DR   PharmGKB; PA29631; -.
DR   VEuPathDB; HostDB:ENSG00000182054; -.
DR   eggNOG; KOG1526; Eukaryota.
DR   GeneTree; ENSGT00390000012547; -.
DR   HOGENOM; CLU_023296_1_1_1; -.
DR   InParanoid; P48735; -.
DR   OMA; IQKRWPL; -.
DR   OrthoDB; 1029485at2759; -.
DR   PhylomeDB; P48735; -.
DR   TreeFam; TF300428; -.
DR   BioCyc; MetaCyc:HS00021-MON; -.
DR   BRENDA; 1.1.1.42; 2681.
DR   PathwayCommons; P48735; -.
DR   Reactome; R-HSA-2151201; Transcriptional activation of mitochondrial biogenesis.
DR   Reactome; R-HSA-71403; Citric acid cycle (TCA cycle).
DR   SABIO-RK; P48735; -.
DR   SignaLink; P48735; -.
DR   SIGNOR; P48735; -.
DR   BioGRID-ORCS; 3418; 12 hits in 1080 CRISPR screens.
DR   ChiTaRS; IDH2; human.
DR   GeneWiki; IDH2; -.
DR   GenomeRNAi; 3418; -.
DR   Pharos; P48735; Tclin.
DR   PRO; PR:P48735; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   RNAct; P48735; protein.
DR   Bgee; ENSG00000182054; Expressed in apex of heart and 208 other tissues.
DR   ExpressionAtlas; P48735; baseline and differential.
DR   Genevisible; P48735; HS.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005759; C:mitochondrial matrix; TAS:Reactome.
DR   GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR   GO; GO:0005777; C:peroxisome; IEA:Ensembl.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IDA:CACAO.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; NAS:ProtInc.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006739; P:NADP metabolic process; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Alternative splicing; Disease variant;
KW   Glyoxylate bypass; Magnesium; Manganese; Metal-binding; Mitochondrion;
KW   NADP; Oxidoreductase; Reference proteome; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..39
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250|UniProtKB:P56574"
FT   CHAIN           40..452
FT                   /note="Isocitrate dehydrogenase [NADP], mitochondrial"
FT                   /id="PRO_0000014420"
FT   BINDING         115..117
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         117
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         122
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         134..140
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         149
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         172
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         291
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         299
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         314
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   BINDING         349..354
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         367
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   SITE            179
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   SITE            251
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250|UniProtKB:P33198"
FT   MOD_RES         45
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         48
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         67
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         69
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         80
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         80
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         106
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         106
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         155
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         166
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         166
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         180
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         180
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         193
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         193
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         199
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         256
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         256
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         263
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         272
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         275
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         280
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         282
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         282
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         384
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         384
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         400
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         413
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000269|PubMed:22416140"
FT   MOD_RES         442
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   VAR_SEQ         1..52
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_056278"
FT   VARIANT         140
FT                   /note="R -> G (in D2HGA2; dbSNP:rs267606870)"
FT                   /evidence="ECO:0000269|PubMed:20847235"
FT                   /id="VAR_065174"
FT   VARIANT         140
FT                   /note="R -> Q (in D2HGA2; dbSNP:rs121913502)"
FT                   /evidence="ECO:0000269|PubMed:20847235"
FT                   /id="VAR_065175"
FT   VARIANT         158
FT                   /note="P -> L (in GLM; somatic mutation)"
FT                   /evidence="ECO:0000269|PubMed:25495392"
FT                   /id="VAR_073181"
FT   VARIANT         162
FT                   /note="P -> S (in GLM; somatic mutation)"
FT                   /evidence="ECO:0000269|PubMed:25495392"
FT                   /id="VAR_073182"
FT   VARIANT         172
FT                   /note="R -> G (in GLM; somatic mutation; reduces enzymatic
FT                   activity; dbSNP:rs1057519906)"
FT                   /evidence="ECO:0000269|PubMed:19228619"
FT                   /id="VAR_073183"
FT   VARIANT         172
FT                   /note="R -> K (in GLM; somatic mutation; reduces enzymatic
FT                   activity; dbSNP:rs121913503)"
FT                   /evidence="ECO:0000269|PubMed:19228619,
FT                   ECO:0000269|PubMed:25495392"
FT                   /id="VAR_073184"
FT   VARIANT         172
FT                   /note="R -> M (in GLM; somatic mutation; reduces enzymatic
FT                   activity; dbSNP:rs121913503)"
FT                   /evidence="ECO:0000269|PubMed:19228619"
FT                   /id="VAR_073185"
FT   VARIANT         172
FT                   /note="R -> S (found in patients with cartilagenous tumors;
FT                   dbSNP:rs1057519736)"
FT                   /evidence="ECO:0000269|PubMed:26161668"
FT                   /id="VAR_076512"
FT   VARIANT         172
FT                   /note="R -> T (found in patients with cartilagenous
FT                   tumors)"
FT                   /evidence="ECO:0000269|PubMed:26161668"
FT                   /id="VAR_076513"
FT   VARIANT         172
FT                   /note="R -> W (found in patients with cartilagenous tumors;
FT                   dbSNP:rs1057519906)"
FT                   /evidence="ECO:0000269|PubMed:26161668"
FT                   /id="VAR_076514"
FT   MUTAGEN         413
FT                   /note="K->A: 44-fold loss in activity."
FT                   /evidence="ECO:0000269|PubMed:22416140"
FT   MUTAGEN         413
FT                   /note="K->Q: 20-fold decrease in Vmax."
FT                   /evidence="ECO:0000269|PubMed:22416140"
FT   MUTAGEN         413
FT                   /note="K->R: No appreciable difference in Km for isocitrate
FT                   and NADP."
FT                   /evidence="ECO:0000269|PubMed:22416140"
FT   CONFLICT        34
FT                   /note="Q -> H (in Ref. 1; CAA49208)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        435
FT                   /note="T -> M (in Ref. 1; CAA49208)"
FT                   /evidence="ECO:0000305"
FT   STRAND          50..54
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           57..69
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   TURN            70..74
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          79..83
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           86..91
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   TURN            92..94
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           95..107
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          108..112
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           135..143
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          146..151
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          169..173
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           177..180
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          182..186
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          190..196
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          205..214
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          216..220
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           225..242
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          246..250
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   TURN            252..254
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           258..273
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           275..280
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          285..289
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           290..299
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          304..308
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           310..324
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          329..335
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   STRAND          342..345
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           352..359
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           369..386
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           389..407
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           413..420
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           422..424
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   TURN            427..429
FT                   /evidence="ECO:0007829|PDB:5I95"
FT   HELIX           434..449
FT                   /evidence="ECO:0007829|PDB:5I95"
SQ   SEQUENCE   452 AA;  50909 MW;  4DDC830AFC06AB52 CRC64;
     MAGYLRVVRS LCRASGSRPA WAPAALTAPT SQEQPRRHYA DKRIKVAKPV VEMDGDEMTR
     IIWQFIKEKL ILPHVDIQLK YFDLGLPNRD QTDDQVTIDS ALATQKYSVA VKCATITPDE
     ARVEEFKLKK MWKSPNGTIR NILGGTVFRE PIICKNIPRL VPGWTKPITI GRHAHGDQYK
     ATDFVADRAG TFKMVFTPKD GSGVKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI
     QKKWPLYMST KNTILKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS
     SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT VTRHYREHQK
     GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQMLEK VCVETVESGA MTKDLAGCIH
     GLSNVKLNEH FLNTTDFLDT IKSNLDRALG RQ
 
 
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