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IDHP_PIG
ID   IDHP_PIG                Reviewed;         421 AA.
AC   P33198;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Isocitrate dehydrogenase [NADP], mitochondrial;
DE            Short=IDH;
DE            EC=1.1.1.42 {ECO:0000269|PubMed:14512428};
DE   AltName: Full=ICD-M;
DE   AltName: Full=IDP;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   Flags: Precursor; Fragment;
GN   Name=IDH2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1627563; DOI=10.1021/bi00142a007;
RA   Haselbeck R.J., Colman R.F., McAlister-Henn L.;
RT   "Isolation and sequence of a cDNA encoding porcine mitochondrial NADP-
RT   specific isocitrate dehydrogenase.";
RL   Biochemistry 31:6219-6223(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 82-91; 59-64; 201-211; 269-278 AND 369-392, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Heart;
RX   PubMed=1869531; DOI=10.1016/s0021-9258(18)98565-0;
RA   Smyth G.E., Colman R.F.;
RT   "Cysteinyl peptides of pig heart NADP-dependent isocitrate dehydrogenase
RT   that are modified upon inactivation by N-ethylmaleimide.";
RL   J. Biol. Chem. 266:14918-14925(1991).
RN   [3]
RP   PROTEIN SEQUENCE OF 383-395.
RX   PubMed=3624273; DOI=10.1016/s0021-9258(18)45250-7;
RA   Ehrlich R.S., Colman R.F.;
RT   "Characterization of an active site peptide modified by the substrate
RT   analogue 3-bromo-2-ketoglutarate on a single chain of dimeric NADP+-
RT   dependent isocitrate dehydrogenase.";
RL   J. Biol. Chem. 262:12614-12619(1987).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP   MUTAGENESIS OF TYR-148 AND LYS-220.
RX   PubMed=14512428; DOI=10.1074/jbc.m303781200;
RA   Kim T.K., Lee P., Colman R.F.;
RT   "Critical role of Lys212 and Tyr140 in porcine NADP-dependent isocitrate
RT   dehydrogenase.";
RL   J. Biol. Chem. 278:49323-49331(2003).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 9-421 IN COMPLEX WITH MANGANESE
RP   AND ISOCITRATE, COFACTOR, AND SUBUNIT.
RX   PubMed=12207025; DOI=10.1074/jbc.m207306200;
RA   Ceccarelli C., Grodsky N.B., Ariyaratne N., Colman R.F., Bahnson B.J.;
RT   "Crystal structure of porcine mitochondrial NADP+-dependent isocitrate
RT   dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme
RT   mechanism.";
RL   J. Biol. Chem. 277:43454-43462(2002).
CC   -!- FUNCTION: Plays a role in intermediary metabolism and energy production
CC       (PubMed:14512428). It may tightly associate or interact with the
CC       pyruvate dehydrogenase complex (PubMed:14512428).
CC       {ECO:0000269|PubMed:14512428}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC         Evidence={ECO:0000269|PubMed:14512428};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:12207025};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:12207025};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000269|PubMed:12207025};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.47 uM for isocitrate {ECO:0000269|PubMed:14512428};
CC         KM=9.85 uM for NADP {ECO:0000269|PubMed:14512428};
CC         KM=0.11 uM for manganese {ECO:0000269|PubMed:14512428};
CC         Vmax=39.6 umol/min/mg enzyme with isocitrate as substrate
CC         {ECO:0000269|PubMed:14512428};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12207025}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305|PubMed:1869531}.
CC   -!- PTM: Acetylation at Lys-382 dramatically reduces catalytic activity.
CC       Deacetylated by SIRT3 (By similarity). {ECO:0000250|UniProtKB:P48735}.
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; M86719; AAA31089.1; -; mRNA.
DR   PIR; A43294; A43294.
DR   PDB; 1LWD; X-ray; 1.85 A; A/B=9-421.
DR   PDBsum; 1LWD; -.
DR   AlphaFoldDB; P33198; -.
DR   SMR; P33198; -.
DR   STRING; 9823.ENSSSCP00000002026; -.
DR   PaxDb; P33198; -.
DR   PeptideAtlas; P33198; -.
DR   PRIDE; P33198; -.
DR   eggNOG; KOG1526; Eukaryota.
DR   HOGENOM; CLU_023296_1_1_1; -.
DR   InParanoid; P33198; -.
DR   BRENDA; 1.1.1.42; 6170.
DR   SABIO-RK; P33198; -.
DR   EvolutionaryTrace; P33198; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Genevisible; P33198; SS.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; ISS:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006739; P:NADP metabolic process; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Direct protein sequencing; Glyoxylate bypass;
KW   Magnesium; Manganese; Metal-binding; Mitochondrion; NADP; Oxidoreductase;
KW   Reference proteome; Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT         <1..8
FT                   /note="Mitochondrion"
FT   CHAIN           9..421
FT                   /note="Isocitrate dehydrogenase [NADP], mitochondrial"
FT                   /id="PRO_0000014422"
FT   BINDING         84..86
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         86
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         91
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         103..109
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         118
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         141
FT                   /ligand="D-threo-isocitrate"
FT                   /ligand_id="ChEBI:CHEBI:15562"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         260
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         268
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         283
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000269|PubMed:12207025,
FT                   ECO:0007744|PDB:1LWD"
FT   BINDING         318..323
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   BINDING         336
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O75874"
FT   SITE            148
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000269|PubMed:14512428"
FT   SITE            220
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000269|PubMed:14512428"
FT   MOD_RES         14
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         17
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         36
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         38
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         49
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         49
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         75
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         75
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         124
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         135
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         135
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         149
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         149
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         162
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         162
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         168
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         225
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         225
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         232
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         241
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         244
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         249
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         251
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         251
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         353
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         353
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         369
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P54071"
FT   MOD_RES         382
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MOD_RES         411
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P48735"
FT   MUTAGEN         148
FT                   /note="Y->F: Large decrease in Vmax and insignificant
FT                   change in KM for isocitrate and NADP."
FT                   /evidence="ECO:0000269|PubMed:14512428"
FT   MUTAGEN         220
FT                   /note="K->Q,Y: Large decrease in Vmax and insignificant
FT                   change in KM for isocitrate and NADP."
FT                   /evidence="ECO:0000269|PubMed:14512428"
FT   NON_TER         1
FT   STRAND          19..23
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           26..38
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   TURN            39..43
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          48..52
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           55..60
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   TURN            61..63
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           64..76
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          77..81
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           89..95
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           104..112
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          114..120
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          137..142
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           146..149
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          151..155
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          157..167
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          174..183
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          185..193
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           194..211
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          215..219
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   TURN            221..223
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           227..242
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           244..249
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          254..258
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           259..268
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          273..277
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           279..293
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          298..304
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   STRAND          311..314
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           321..328
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           338..355
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           358..376
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           382..389
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   TURN            396..398
FT                   /evidence="ECO:0007829|PDB:1LWD"
FT   HELIX           403..419
FT                   /evidence="ECO:0007829|PDB:1LWD"
SQ   SEQUENCE   421 AA;  47526 MW;  14862D47C00A053F CRC64;
     ARAAARHYAD QRIKVAKPVV EMDGDEMTRI IWQFIKEKLI LPHVDVQLKY FDLGLPNRDQ
     TNDQVTIDSA LATQKYSVAV KCATITPDEA RVEEFKLKKM WKSPNGTIRN ILGGTVFREP
     IICKNIPRLV PGWTKPITIG RHAHGDQYKA TDFVVDRAGT FKIVFTPKDG SSAKQWEVYN
     FPAGGVGMGM YNTDESISGF AHSCFQYAIQ KKWPLYMSTK NTILKAYDGR FKDIFQEIFE
     KHYKTDFDKY KIWYEHRLID DMVAQVLKSS GGFVWACKNY DGDVQSDILA QGFGSLGLMT
     SVLVCPDGKT IEAEAAHGTV TRHYREHQKG RPTSTNPIAS IFAWTRGLEH RGKLDGNQDL
     IRFAQTLEKV CVETVESGAM TKDLAGCIHG LSNVKLNEHF LNTSDFLDTI KSNLDRALGR
     Q
 
 
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