IDH_STRMU
ID IDH_STRMU Reviewed; 393 AA.
AC Q59940; Q59927;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2002, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Isocitrate dehydrogenase [NADP];
DE Short=IDH;
DE EC=1.1.1.42;
DE AltName: Full=IDP;
DE AltName: Full=NADP(+)-specific ICDH;
DE AltName: Full=Oxalosuccinate decarboxylase;
GN Name=icd; Synonyms=idh; OrderedLocusNames=SMU_672;
OS Streptococcus mutans serotype c (strain ATCC 700610 / UA159).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=210007;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JH1005;
RA Cvitkovitch D.G., Gutierrez J.A., Bleiweis A.S.;
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700610 / UA159;
RX PubMed=12397186; DOI=10.1073/pnas.172501299;
RA Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B.,
RA Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S.,
RA Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.;
RT "Genome sequence of Streptococcus mutans UA159, a cariogenic dental
RT pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-224.
RC STRAIN=JH1005;
RA Gutierrez J.A., Crowley P.J., Brown D.P., Hillman J.D., Youngman P.,
RA Bleiweis A.S.;
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit. {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC dehydrogenases family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U62799; AAC44826.1; -; Genomic_DNA.
DR EMBL; AE014133; AAN58406.1; -; Genomic_DNA.
DR EMBL; U48886; AAC44503.1; -; Genomic_DNA.
DR RefSeq; NP_721100.1; NC_004350.2.
DR RefSeq; WP_002261869.1; NC_004350.2.
DR AlphaFoldDB; Q59940; -.
DR SMR; Q59940; -.
DR STRING; 210007.SMU_672; -.
DR PRIDE; Q59940; -.
DR EnsemblBacteria; AAN58406; AAN58406; SMU_672.
DR KEGG; smu:SMU_672; -.
DR PATRIC; fig|210007.7.peg.597; -.
DR eggNOG; COG0538; Bacteria.
DR HOGENOM; CLU_031953_7_1_9; -.
DR OMA; CVRPCRY; -.
DR PhylomeDB; Q59940; -.
DR Proteomes; UP000002512; Chromosome.
DR GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR InterPro; IPR004439; Isocitrate_DH_NADP_dimer_prok.
DR InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR PANTHER; PTHR43504; PTHR43504; 2.
DR Pfam; PF00180; Iso_dh; 1.
DR SMART; SM01329; Iso_dh; 1.
DR TIGRFAMs; TIGR00183; prok_nadp_idh; 1.
DR PROSITE; PS00470; IDH_IMDH; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP;
KW Oxidoreductase; Phosphoprotein; Reference proteome;
KW Tricarboxylic acid cycle.
FT CHAIN 1..393
FT /note="Isocitrate dehydrogenase [NADP]"
FT /id="PRO_0000083568"
FT BINDING 102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 108
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 118
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 283
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT SITE 149
FT /note="Critical for catalysis"
FT /evidence="ECO:0000250"
FT SITE 219
FT /note="Critical for catalysis"
FT /evidence="ECO:0000250"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT CONFLICT 67
FT /note="N -> K (in Ref. 1; AAC44826)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="R -> C (in Ref. 1; AAC44826)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 393 AA; 43141 MW; 0F19F22A93D9DCD6 CRC64;
MAEKVSFEEG KLQVPDKPVI PYIEGDGVGQ DIWKNAQIVF DKAIAKVYGG HKQVIWREVL
AGKKAYNETG NWLPNETLEI IKTHLLAIKG PLETPVGGGI RSLNVALRQE LDLFACVRPV
RYFKGVPSPL KHPEKTAITI FRENTEDIYA GIEWNAGTAE VQKVINFLQD DMQVKKIRFP
KSSSIGIKPI SIEGSQRLIR AAIEYALANN LTKVTLVHKG NIQKFTEGGF RKWGYELAKR
EYAAELASGQ LVVDDIIADN FLQQILLKPE RFDVVALTNL NGDYASDALA AQVGGIGISP
GANINYQTGH AIFEATHGTA PDIAGQDLAN PSSVLLSGCM LFDYIGWSKV SDLIMKAVEK
AIANGQVTID FAKELGVEAL TTRQFSEVLL TYL