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IDH_THET8
ID   IDH_THET8               Reviewed;         496 AA.
AC   P33197; Q5SI45;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Isocitrate dehydrogenase [NADP];
DE            Short=IDH;
DE            EC=1.1.1.42;
DE   AltName: Full=IDP;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=Oxalosuccinate decarboxylase;
GN   Name=icd; OrderedLocusNames=TTHA1535;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1539996; DOI=10.1128/aem.58.1.93-98.1992;
RA   Miyazaki K., Eguchi H., Yamagishi A., Wakagi T., Oshima T.;
RT   "Molecular cloning of the isocitrate dehydrogenase gene of an extreme
RT   thermophile, Thermus thermophilus HB8.";
RL   Appl. Environ. Microbiol. 58:93-98(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; M94317; AAA27492.1; -; Genomic_DNA.
DR   EMBL; AP008226; BAD71358.1; -; Genomic_DNA.
DR   RefSeq; WP_011228743.1; NC_006461.1.
DR   RefSeq; YP_144801.1; NC_006461.1.
DR   PDB; 2D1C; X-ray; 1.80 A; A/B=1-496.
DR   PDBsum; 2D1C; -.
DR   AlphaFoldDB; P33197; -.
DR   SMR; P33197; -.
DR   STRING; 300852.55772917; -.
DR   EnsemblBacteria; BAD71358; BAD71358; BAD71358.
DR   GeneID; 3168917; -.
DR   KEGG; ttj:TTHA1535; -.
DR   PATRIC; fig|300852.9.peg.1509; -.
DR   eggNOG; COG0473; Bacteria.
DR   HOGENOM; CLU_031953_1_3_0; -.
DR   OMA; NGDDIGH; -.
DR   PhylomeDB; P33197; -.
DR   BRENDA; 1.1.1.42; 2305.
DR   EvolutionaryTrace; P33197; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR014273; Isocitrate_DH_aproteobac-typ.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   InterPro; IPR040978; TT1725.
DR   PANTHER; PTHR11835:SF43; PTHR11835:SF43; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   Pfam; PF18324; TT1725; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP;
KW   Oxidoreductase; Phosphoprotein; Reference proteome;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..496
FT                   /note="Isocitrate dehydrogenase [NADP]"
FT                   /id="PRO_0000083571"
FT   BINDING         104
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         224
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         224
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         248
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         252
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   SITE            144
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250"
FT   SITE            191
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         98
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        201..202
FT                   /note="TL -> P (in Ref. 1; AAA27492)"
FT                   /evidence="ECO:0000305"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          11..14
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          20..25
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           31..44
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          49..53
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           58..61
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            62..64
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           71..80
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          82..85
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          92..96
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           99..106
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          111..117
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            125..128
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          132..138
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          140..142
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           143..145
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          148..152
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          155..163
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           164..180
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          185..190
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            192..194
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           198..210
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          216..222
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           223..232
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           234..236
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          238..242
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           244..255
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            256..259
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           261..263
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          265..269
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          274..280
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            284..288
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           295..307
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           311..327
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           333..336
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            338..340
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           344..353
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   TURN            354..356
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          360..362
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           375..377
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          387..397
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           402..413
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          416..425
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          428..432
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          442..450
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          452..454
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   HELIX           458..469
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          472..483
FT                   /evidence="ECO:0007829|PDB:2D1C"
FT   STRAND          486..489
FT                   /evidence="ECO:0007829|PDB:2D1C"
SQ   SEQUENCE   496 AA;  54439 MW;  BF544C3F772E1AE9 CRC64;
     MPLITTETGK KMHVLEDGRK LITVIPGDGI GPECVEATLK VLEAAKAPLA YEVREAGASV
     FRRGIASGVP QETIESIRKT RVVLKGPLET PVGYGEKSAN VTLRKLFETY ANVRPVREFP
     NVPTPYAGRG IDLVVVRENV EDLYAGIEHM QTPSVAQTLK LISWKGSEKI VRFAFELARA
     EGRKKVHCAT KSNIMKLAEG TLKRAFEQVA QEYPDIEAVH IIVDNAAHQL VKRPEQFEVI
     VTTNMNGDIL SDLTSGLIGG LGFAPSANIG NEVAIFEAVH GSAPKYAGKN VINPTAVLLS
     AVMMLRYLEE FATADLIENA LLYTLEEGRV LTGDVVGYDR GAKTTEYTEA IIQNLGKTPR
     KTQVRGYKPF RLPQVDGAIA PIVPRSRRVV GVDVFVETNL LPEALGKALE DLAAGTPFRL
     KMISNRGTQV YPPTGGLTDL VDHYRCRFLY TGEGEAKDPE ILDLVSRVAS RFRWMHLEKL
     QEFDGEPGFT KAQGED
 
 
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