ID   APC4_DICDI              Reviewed;         837 AA.
AC   Q54NI1;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Anaphase-promoting complex subunit 4;
DE            Short=APC4;
GN   Name=anapc4; Synonyms=apc4; ORFNames=DDB_G0285223;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC       (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC       that controls progression through mitosis and the G1 phase of the cell
CC       cycle. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: The APC/C is composed of at least 13 subunits that stay
CC       tightly associated throughout the cell cycle: anapc1, anapc2, anapc3,
CC       anapc4, anapc5, anapc6, anapc7, anapc8, anapc10, anapc11, cdc20, cdc26
CC       and cdh1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the APC4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000076; EAL64823.1; -; Genomic_DNA.
DR   RefSeq; XP_638336.1; XM_633244.1.
DR   AlphaFoldDB; Q54NI1; -.
DR   STRING; 44689.DDB0234268; -.
DR   PaxDb; Q54NI1; -.
DR   EnsemblProtists; EAL64823; EAL64823; DDB_G0285223.
DR   GeneID; 8625004; -.
DR   KEGG; ddi:DDB_G0285223; -.
DR   dictyBase; DDB_G0285223; anapc4.
DR   eggNOG; KOG4640; Eukaryota.
DR   HOGENOM; CLU_339626_0_0_1; -.
DR   InParanoid; Q54NI1; -.
DR   OMA; FLNIFHI; -.
DR   PhylomeDB; Q54NI1; -.
DR   Reactome; R-DDI-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR   Reactome; R-DDI-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR   Reactome; R-DDI-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR   Reactome; R-DDI-174154; APC/C:Cdc20 mediated degradation of Securin.
DR   Reactome; R-DDI-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR   Reactome; R-DDI-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR   Reactome; R-DDI-176407; Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
DR   Reactome; R-DDI-176408; Regulation of APC/C activators between G1/S and early anaphase.
DR   Reactome; R-DDI-176409; APC/C:Cdc20 mediated degradation of mitotic proteins.
DR   Reactome; R-DDI-176412; Phosphorylation of the APC/C.
DR   Reactome; R-DDI-179409; APC-Cdc20 mediated degradation of Nek2A.
DR   Reactome; R-DDI-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DDI-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR   Reactome; R-DDI-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR   Reactome; R-DDI-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q54NI1; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR   GO; GO:0034399; C:nuclear periphery; IBA:GO_Central.
DR   GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR   GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR024789; APC4.
DR   InterPro; IPR024790; APC4_long_dom.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR13260; PTHR13260; 1.
DR   Pfam; PF12896; ANAPC4; 1.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome;
KW   Ubl conjugation pathway.
FT   CHAIN           1..837
FT                   /note="Anaphase-promoting complex subunit 4"
FT                   /id="PRO_0000328279"
FT   REGION          59..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          547..588
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   837 AA;  96767 MW;  6C55CDBFC495D645 CRC64;
     MKDFVLLNDK ILPNDVKCYS CCTTMDLIAL VTKDDQIVIH RFLTWQKLFT INHMSTTIND
     NNTNNNNNNN NNNNNNNNDN NNDNDKSNKS IVSIQWSPNG KMISIGCEDG SIFIYNIENA
     KLINKSHNHK HPIHKLAWIK EVSQQRSQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQQQNKNGS TKCNNNYVSP PLFLSQIKQN MNLFPSISYY FENSKEENIY LGGDIYDRPF
     DILICCDSIG VISLLAFGLF KIVTIDLLSL LKQKYSNTHF LIKPSKSLKI LDITLTESLN
     KLSVMIETDN GLFLSVTIDT SILLEKRNEI HEISLQYFLL FQLQQSLDIH IKEITEKWKE
     TQQQLSTKWV EFEKVLSDYG FSSSIEQELI DLLMCGVPSP PTNQFIVNNI NIKKLKLTES
     NCNSIREILI KYILPSFLNI FHIITVLHNN SLENDGYKGL LDTNTVKNIL DYCGSFGMRL
     QSLETLICGI ESHYTSFFSW LYKVQCTLNE IQPDRKLSLP FNELSIMSLL KKGLKFDLLF
     STSTLFSSSS SSSPSSPPSN NNSPTFSSSS SINNNNNNNN NELNQSGNIG FSNNNENLEF
     QYKDLKGNFH DLFKDFSSKI FETFNEITIV LPSLFKFENV IPFCLYDKND TSVSHKFNCS
     LISHPNDTIY LSIYTTLSNS NRLFICKRED KLNWSFTCYQ LNEKYSILDC KFYNNSSLMA
     LVSEDIIKAN QKKSTRKNTY LKQYQYKTDD NDDSDNREYI KLDVNIPQST ILLDLLDSFK
     SREIILKSRI SPITFELSTS RKISATFIGK RRVALYDLAE DEEEEEEGEE EDICLVR