APC4_YEAST
ID APC4_YEAST Reviewed; 652 AA.
AC Q04601; D6VSA3;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Anaphase-promoting complex subunit 4;
GN Name=APC4; OrderedLocusNames=YDR118W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP PROTEIN SEQUENCE OF 38-44 AND 337-343, SUBUNIT, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=9469814; DOI=10.1126/science.279.5354.1216;
RA Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J.,
RA Mann M., Nasmyth K.;
RT "Mass spectrometric analysis of the anaphase-promoting complex from yeast:
RT identification of a subunit related to cullins.";
RL Science 279:1216-1219(1998).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C is thought to confer substrate specificity and, in the
CC presence of ubiquitin-conjugating E2 enzymes, it catalyzes the
CC formation of protein-ubiquitin conjugates that are subsequently
CC degraded by the 26S proteasome. In early mitosis, the APC/C is
CC activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5,
CC and other anaphase inhibitory proteins for proteolysis, thereby
CC triggering the separation of sister chromatids at the metaphase-to-
CC anaphase transition. In late mitosis and in G1, degradation of CLB5
CC allows activation of the APC/C by CDH1, which is needed to destroy
CC CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and
CC creating the low CDK state necessary for cytokinesis and for reforming
CC prereplicative complexes in G1 prior to another round of replication.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 13 subunits that stay
CC tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5,
CC APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.
CC {ECO:0000269|PubMed:9469814}.
CC -!- INTERACTION:
CC Q04601; Q08683: APC5; NbExp=7; IntAct=EBI-32842, EBI-35371;
CC Q04601; P40577: MND2; NbExp=5; IntAct=EBI-32842, EBI-25433;
CC Q04601; Q12379: SWM1; NbExp=5; IntAct=EBI-32842, EBI-33330;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1332 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z48758; CAA88671.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11963.1; -; Genomic_DNA.
DR PIR; S52683; S52683.
DR RefSeq; NP_010403.3; NM_001180426.3.
DR AlphaFoldDB; Q04601; -.
DR BioGRID; 32174; 46.
DR ComplexPortal; CPX-756; Anaphase-Promoting core complex.
DR ComplexPortal; CPX-760; Anaphase-Promoting Complex, CDC20 variant.
DR ComplexPortal; CPX-761; Anaphase-Promoting Complex, CDH1 variant.
DR ComplexPortal; CPX-762; Anaphase-Promoting complex AMA1 variant.
DR DIP; DIP-1132N; -.
DR IntAct; Q04601; 13.
DR MINT; Q04601; -.
DR STRING; 4932.YDR118W; -.
DR MaxQB; Q04601; -.
DR PaxDb; Q04601; -.
DR PRIDE; Q04601; -.
DR EnsemblFungi; YDR118W_mRNA; YDR118W; YDR118W.
DR GeneID; 851696; -.
DR KEGG; sce:YDR118W; -.
DR SGD; S000002525; APC4.
DR VEuPathDB; FungiDB:YDR118W; -.
DR eggNOG; KOG4640; Eukaryota.
DR HOGENOM; CLU_030192_0_0_1; -.
DR InParanoid; Q04601; -.
DR OMA; PACERII; -.
DR BioCyc; YEAST:G3O-29718-MON; -.
DR Reactome; R-SCE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q04601; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04601; protein.
DR GO; GO:0005680; C:anaphase-promoting complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0034399; C:nuclear periphery; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IDA:ComplexPortal.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IDA:ComplexPortal.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:InterPro.
DR InterPro; IPR024789; APC4.
DR InterPro; IPR024790; APC4_long_dom.
DR PANTHER; PTHR13260; PTHR13260; 1.
DR Pfam; PF12896; ANAPC4; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Direct protein sequencing; Mitosis;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..652
FT /note="Anaphase-promoting complex subunit 4"
FT /id="PRO_0000064623"
SQ SEQUENCE 652 AA; 75256 MW; CC7E3818FFD6AF25 CRC64;
MSSPINDYFI DYNPLFPIFA TRIAKGLAIY RVSDHARLAV IPIRNINLVA NYDWDTTTGK
FLSIFFKDGT IRIHDIFKDG RLVSFLRIPS TKISKGIWDR IPLRYEPNNR DFACNIIDDL
PKLIRFVKDS KRINIVPYTQ PNSLWRGPDE DDLDSNEKLD VHVVFNEGND KITVFFNGDY
AVFLSVDNIE NENSLKSIIK VQDGFYQCFY EDGTVQTLNL GPLLQSKSSV NLLNYIMVIK
ELIGYMLTHL EFINRELATP YLDFVKRLCD EAYGYGKLKS ELEALFLLGE ISCDLEDWLC
NSVGEKNFKR WKYLGCEAYQ KTVQILTLIF VPACERIIIY VEKLRAILQA FSIQNKLSYT
SDLTAVEVLL KSSQKLLTMT LNSIIGLGRD ETLFEKFFIW FNDRLHEALD EDYKLKFQFE
DDLYFGYDLL SYFDRILSKK GTEPSSIIDV KLYRDLINSM SDMEKDIAQS NVNSHIQQHI
LVDLKTDVFA QKYPSSQINL LDAIKLPKHN YIVYLIQVTK HNSAQEPFSE ENKKKLYIGT
LKDENLGIIS KESSVKIPAL FKSYRLSSTR FVPNRVHSLL RDIGLSDSNY HSSHVTDYRG
ENYENEEDDG TIAIPAYIRE NRENDDFIAC TAKVSVDGRS ASLVFPKEKQ NV
