APC5B_XENLA
ID APC5B_XENLA Reviewed; 152 AA.
AC Q6DE18;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Actin-related protein 2/3 complex subunit 5-B {ECO:0000305};
DE AltName: Full=Actin-related protein 2/3 complex subunit 5-like {ECO:0000305};
GN Name=arpc5-b; Synonyms=arpc5l {ECO:0000312|Xenbase:XB-GENE-490528};
GN ORFNames=XELAEV_18038444mg {ECO:0000312|EMBL:OCT67163.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J;
RX PubMed=27762356; DOI=10.1038/nature19840;
RA Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL Nature 538:336-343(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ARP2/3 COMPLEX, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29925947; DOI=10.1038/s41586-018-0237-5;
RA Schrank B.R., Aparicio T., Li Y., Chang W., Chait B.T., Gundersen G.G.,
RA Gottesman M.E., Gautier J.;
RT "Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.";
RL Nature 559:61-66(2018).
CC -!- FUNCTION: Component of the Arp2/3 complex, a multiprotein complex that
CC mediates actin polymerization upon stimulation by nucleation-promoting
CC factor (NPF) (By similarity). The Arp2/3 complex mediates the formation
CC of branched actin networks in the cytoplasm, providing the force for
CC cell motility (By similarity). In addition to its role in the
CC cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin
CC polymerization in the nucleus, thereby regulating gene transcription
CC and repair of damaged DNA (Probable). The Arp2/3 complex promotes
CC homologous recombination (HR) repair in response to DNA damage by
CC promoting nuclear actin polymerization, leading to drive motility of
CC double-strand breaks (DSBs) (By similarity).
CC {ECO:0000250|UniProtKB:O15511, ECO:0000250|UniProtKB:Q68FI4,
CC ECO:0000305|PubMed:29925947}.
CC -!- SUBUNIT: Component of the Arp2/3 complex composed of actr2/arp2,
CC actr3/arp3, arpc1 (arpc1a or arpc1b), arpc2, arpc3, arpc4 and arpc5.
CC {ECO:0000269|PubMed:29925947}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q68FI4}. Cell projection
CC {ECO:0000250|UniProtKB:Q68FI4}. Nucleus {ECO:0000269|PubMed:29925947}.
CC -!- SIMILARITY: Belongs to the ARPC5 family. {ECO:0000305}.
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DR EMBL; CM004480; OCT67163.1; -; Genomic_DNA.
DR EMBL; BC077328; AAH77328.1; -; mRNA.
DR RefSeq; NP_001086702.1; NM_001093233.1.
DR AlphaFoldDB; Q6DE18; -.
DR SMR; Q6DE18; -.
DR MaxQB; Q6DE18; -.
DR DNASU; 446537; -.
DR GeneID; 446537; -.
DR KEGG; xla:446537; -.
DR CTD; 446537; -.
DR Xenbase; XB-GENE-490528; arpc5l.L.
DR OMA; SQIQGRN; -.
DR OrthoDB; 1565115at2759; -.
DR Proteomes; UP000186698; Chromosome 8L.
DR Bgee; 446537; Expressed in neurula embryo and 19 other tissues.
DR GO; GO:0005885; C:Arp2/3 protein complex; IDA:UniProtKB.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0035861; C:site of double-strand break; IDA:UniProtKB.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR Gene3D; 1.25.40.190; -; 1.
DR InterPro; IPR006789; ARPC5.
DR InterPro; IPR036743; ARPC5_sf.
DR InterPro; IPR030075; ARPC5L.
DR PANTHER; PTHR12644; PTHR12644; 1.
DR PANTHER; PTHR12644:SF2; PTHR12644:SF2; 1.
DR Pfam; PF04699; P16-Arc; 1.
DR PIRSF; PIRSF039096; p16-ARC; 1.
DR SUPFAM; SSF69103; SSF69103; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Cell projection; Cytoplasm; Cytoskeleton; Nucleus;
KW Reference proteome.
FT CHAIN 1..152
FT /note="Actin-related protein 2/3 complex subunit 5-B"
FT /id="PRO_0000445565"
FT REGION 21..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 152 AA; 17138 MW; 848F677B00162BB7 CRC64;
MAKNTLSSRF RKVDIDEYDE NKFVDDQLQE EPAEPQGPDE AEVDSLIRQG ELLRAFQSAL
RNSPVNSKNQ VAKERTQAIV LKVLTSFKSN EIEKAVNTLD PNGIDLLMKY IYKGFEKPTE
NSSAILLQWH EKAFVIGGLG SIVRVLTSRK TV
