ID   IDLP_ECOLI              Reviewed;          27 AA.
AC   P0DPC6; A0A385XJQ3;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=iraD leader peptide;
GN   Name=idlP; Synonyms=orf27 {ECO:0000303|PubMed:28851853};
GN   OrderedLocusNames=b4722;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   IDENTIFICATION, FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / CF7789;
RX   PubMed=28851853; DOI=10.1128/mbio.01355-17;
RA   Park H., McGibbon L.C., Potts A.H., Yakhnin H., Romeo T., Babitzke P.;
RT   "Translational repression of the RpoS antiadapter IraD by CsrA is mediated
RT   via translational coupling to a short upstream open reading frame.";
RL   MBio 8:0-0(2017).
CC   -!- FUNCTION: A short protein whose stop codon overlaps with the start
CC       codon of downstream iraD; its mRNA secondary structure is predicted to
CC       fold and sequester the Shine-Dalgarno sequence of iraD. When this
CC       protein is expressed the downstream iraD is also expressed due to
CC       ribosomal coupling (PubMed:28851853). {ECO:0000269|PubMed:28851853}.
CC   -!- INDUCTION: Expressed at high levels during late logarithmic and
CC       stationary growth (at protein level) (PubMed:28851853). Translation is
CC       repressed by CsrA (PubMed:28851853). {ECO:0000269|PubMed:28851853}.
CC   -!- DISRUPTION PHENOTYPE: Decreased expression of downstream gene iraD
CC       (PubMed:28851853). {ECO:0000269|PubMed:28851853}.
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DR   EMBL; U00096; AYC08260.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0DPC6; -.
DR   EnsemblBacteria; AYC08260; AYC08260; b4722.
DR   BioCyc; EcoCyc:MON0-4390; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Leader peptide; Reference proteome; Translation regulation.
FT   CHAIN           1..27
FT                   /note="iraD leader peptide"
FT                   /id="PRO_0000442644"
SQ   SEQUENCE   27 AA;  3016 MW;  AB77D4EAE857E6B7 CRC64;
     MENEHQYSGA RCSGQAAYVA KRQECAK