IDNT_ECOLI
ID IDNT_ECOLI Reviewed; 439 AA.
AC P39344; Q2M644;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Gnt-II system L-idonate transporter;
DE Short=L-Ido transporter;
DE AltName: Full=5-keto-D-gluconate transporter;
GN Name=idnT; Synonyms=gntW, yjgT; OrderedLocusNames=b4265, JW4222;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=9658018; DOI=10.1128/jb.180.14.3704-3710.1998;
RA Bausch C., Peekhaus N., Utz C., Blais T., Murray E., Lowary T., Conway T.;
RT "Sequence analysis of the GntII (subsidiary) system for gluconate
RT metabolism reveals a novel pathway for L-idonic acid catabolism in
RT Escherichia coli.";
RL J. Bacteriol. 180:3704-3710(1998).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP FUNCTION AS A 5-KETO-D-GLUCONATE TRANSPORTER, ROLE IN 5-KETO-D-GLUCONATE
RP UTILIZATION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=17088549; DOI=10.1073/pnas.0603364103;
RA Reed J.L., Patel T.R., Chen K.H., Joyce A.R., Applebee M.K., Herring C.D.,
RA Bui O.T., Knight E.M., Fong S.S., Palsson B.O.;
RT "Systems approach to refining genome annotation.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:17480-17484(2006).
CC -!- FUNCTION: Transports L-idonate, D-gluconate and 5-keto-D-gluconate,
CC from the periplasm across the inner membrane.
CC {ECO:0000269|PubMed:17088549, ECO:0000269|PubMed:9658018}.
CC -!- PATHWAY: Carbohydrate acid metabolism; L-idonate degradation.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Up-regulated by 5-keto-D-gluconate.
CC {ECO:0000269|PubMed:17088549}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth rate when grown on 5-keto-D-
CC gluconate as sole carbon source. {ECO:0000269|PubMed:17088549}.
CC -!- SIMILARITY: Belongs to the GntP permease family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U14003; AAA97162.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77222.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78262.1; -; Genomic_DNA.
DR PIR; S56491; S56491.
DR RefSeq; NP_418686.1; NC_000913.3.
DR RefSeq; WP_001128335.1; NZ_LN832404.1.
DR AlphaFoldDB; P39344; -.
DR BioGRID; 4262729; 6.
DR DIP; DIP-10014N; -.
DR IntAct; P39344; 1.
DR STRING; 511145.b4265; -.
DR TCDB; 2.A.8.1.2; the gluconate:h(+) symporter (gntp) family.
DR PaxDb; P39344; -.
DR PRIDE; P39344; -.
DR DNASU; 948798; -.
DR EnsemblBacteria; AAC77222; AAC77222; b4265.
DR EnsemblBacteria; BAE78262; BAE78262; BAE78262.
DR GeneID; 948798; -.
DR KEGG; ecj:JW4222; -.
DR KEGG; eco:b4265; -.
DR PATRIC; fig|1411691.4.peg.2438; -.
DR EchoBASE; EB2428; -.
DR eggNOG; COG2610; Bacteria.
DR HOGENOM; CLU_027949_0_0_6; -.
DR InParanoid; P39344; -.
DR OMA; LMRIGIP; -.
DR PhylomeDB; P39344; -.
DR BioCyc; EcoCyc:YJGT-MON; -.
DR BioCyc; MetaCyc:YJGT-MON; -.
DR UniPathway; UPA00793; -.
DR PRO; PR:P39344; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015128; F:gluconate transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015568; F:L-idonate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0008643; P:carbohydrate transport; IDA:EcoCyc.
DR GO; GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0035429; P:gluconate transmembrane transport; IBA:GO_Central.
DR GO; GO:0046183; P:L-idonate catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015726; P:L-idonate transmembrane transport; IDA:EcoCyc.
DR InterPro; IPR003474; Glcn_transporter.
DR PANTHER; PTHR30354; PTHR30354; 1.
DR Pfam; PF02447; GntP_permease; 1.
DR PIRSF; PIRSF002746; Gluconate_transporter; 1.
DR TIGRFAMs; TIGR00791; gntP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Gluconate utilization; Membrane;
KW Reference proteome; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..439
FT /note="Gnt-II system L-idonate transporter"
FT /id="PRO_0000061940"
FT TOPO_DOM 1
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..51
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..254
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..326
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 439 AA; 46041 MW; EA6CBCA88D6C042C CRC64;
MPLIIIAAGV ALLLILMIGF KVNGFIALVL VAAVVGFAEG MDAQAVLHSI QNGIGSTLGG
LAMILGFGAM LGKLISDTGA AQRIATTLIA TFGKKRVQWA LVITGLVVGL AMFFEVGFVL
LLPLVFTIVA SSGLPLLYVG VPMVAALSVT HCFLPPHPGP TAIATIFEAN LGTTLLYGFI
ITIPTVIVAG PLFSKLLTRF EKAPPEGLFN PHLFSEEEMP SFWNSIFAAV IPVILMAIAA
VCEITLPKTN TVRLFFEFVG NPAVALFIAI VIAIFTLGRR NGRTIEQIMD IIGDSIGAIA
MIVFIIAGGG AFKQVLVDSG VGHYISHLMT GTTLSPLLMC WTVAALLRIA LGSATVAAIT
TAGVVLPIIN VTHADPALMV LATGAGSVIA SHVNDPGFWL FKGYFNLTVG ETLRTWTVME
TLISIMGLLG VLAINAVLH
