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IDS3_HORVU
ID   IDS3_HORVU              Reviewed;         339 AA.
AC   Q40062; Q40063; Q9LU11;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 3.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=2'-deoxymugineic-acid 2'-dioxygenase {ECO:0000303|PubMed:11346963};
DE            EC=1.14.11.24 {ECO:0000269|PubMed:11346963};
DE   AltName: Full=Protein iron deficiency-specific 3 {ECO:0000303|PubMed:11117263, ECO:0000303|PubMed:8019781};
GN   Name=IDS3 {ECO:0000303|PubMed:11117263, ECO:0000303|PubMed:8019781};
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=8019781;
RA   Nakanishi H., Okumura N., Umehara Y., Nishizawa N.-K., Chino M., Mori S.;
RT   "Expression of a gene specific for iron deficiency (Ids3) in the roots of
RT   Hordeum vulgare.";
RL   Plant Cell Physiol. 34:401-410(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND INDUCTION BY IRON
RP   DEFICIENCY.
RC   STRAIN=cv. Igri, and cv. NK 1558; TISSUE=Root;
RX   PubMed=11117263; DOI=10.1023/a:1006491521586;
RA   Nakanishi H., Yamaguchi H., Sasakuma T., Nishizawa N.K., Mori S.;
RT   "Two dioxygenase genes, Ids3 and Ids2, from Hordeum vulgare are involved in
RT   the biosynthesis of mugineic acid family phytosiderophores.";
RL   Plant Mol. Biol. 44:199-207(2000).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-12; 80-97 AND 172-191, AND INDUCTION.
RX   PubMed=9489019; DOI=10.1104/pp.116.2.725;
RA   Suzuki K., Itai R., Suzuki K., Nakanishi H., Nishizawa N.K., Yoshimura E.,
RA   Mori S.;
RT   "Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by
RT   iron deficiency in barley roots.";
RL   Plant Physiol. 116:725-732(1998).
RN   [4]
RP   FUNCTION, INDUCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=11346963; DOI=10.1007/s004250000453;
RA   Kobayashi T., Nakanishi H., Takahashi M., Kawasaki S., Nishizawa N.-K.,
RA   Mori S.;
RT   "In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that
RT   converts 2'-deoxymugineic acid to mugineic acid in transgenic rice.";
RL   Planta 212:864-871(2001).
RN   [5]
RP   FUNCTION.
RX   PubMed=17478636; DOI=10.1104/pp.107.096388;
RA   Patterson J., Ford K., Cassin A., Natera S., Bacic A.;
RT   "Increased abundance of proteins involved in phytosiderophore production in
RT   boron-tolerant barley.";
RL   Plant Physiol. 144:1612-1631(2007).
CC   -!- FUNCTION: Involved in the biosynthesis of mugineic acid family of
CC       phytosiderophores. Hydroxylates the C-2' positions of 2'-deoxymugineic
CC       acid (DMA) and 3-epihydroxymugineic acid (epiHDMA). May be involved in
CC       boron tolerance. {ECO:0000269|PubMed:11117263,
CC       ECO:0000269|PubMed:11346963, ECO:0000269|PubMed:17478636}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2'-deoxymugineate + 2-oxoglutarate + O2 = CO2 + mugineate +
CC         succinate; Xref=Rhea:RHEA:12200, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC         ChEBI:CHEBI:58487, ChEBI:CHEBI:77826; EC=1.14.11.24;
CC         Evidence={ECO:0000269|PubMed:11346963};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12201;
CC         Evidence={ECO:0000269|PubMed:11346963};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU00805};
CC   -!- COFACTOR:
CC       Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC         Evidence={ECO:0000250|UniProtKB:Q5W726};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Up-regulated by iron deficiency and down-regulated by iron.
CC       Not induced by anaerobic conditions. {ECO:0000269|PubMed:11117263,
CC       ECO:0000269|PubMed:11346963, ECO:0000269|PubMed:8019781,
CC       ECO:0000269|PubMed:9489019}.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. {ECO:0000305}.
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DR   EMBL; AB024058; BAA75493.1; -; mRNA.
DR   EMBL; AB024007; BAB07798.1; -; Genomic_DNA.
DR   EMBL; D37796; BAA07042.1; -; Genomic_DNA.
DR   PIR; T05743; T05743.
DR   PIR; T05903; T05903.
DR   AlphaFoldDB; Q40062; -.
DR   SMR; Q40062; -.
DR   EnsemblPlants; HORVU.MOREX.r2.4HG0341060.1; HORVU.MOREX.r2.4HG0341060.1; HORVU.MOREX.r2.4HG0341060.
DR   Gramene; HORVU.MOREX.r2.4HG0341060.1; HORVU.MOREX.r2.4HG0341060.1; HORVU.MOREX.r2.4HG0341060.
DR   KEGG; ag:BAA75493; -.
DR   BioCyc; MetaCyc:MON-13953; -.
DR   BRENDA; 1.14.11.24; 2687.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033760; F:2'-deoxymugineic-acid 2'-dioxygenase activity; IDA:UniProtKB.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0010041; P:response to iron(III) ion; IDA:UniProtKB.
DR   Gene3D; 2.60.120.330; -; 1.
DR   InterPro; IPR026992; DIOX_N.
DR   InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR   InterPro; IPR027443; IPNS-like_sf.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR   Pfam; PF14226; DIOX_N; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Dioxygenase; Direct protein sequencing; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN           1..339
FT                   /note="2'-deoxymugineic-acid 2'-dioxygenase"
FT                   /id="PRO_0000389553"
FT   DOMAIN          184..284
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   BINDING         209
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   BINDING         211
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   BINDING         265
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   BINDING         275
FT                   /ligand="2-oxoglutarate"
FT                   /ligand_id="ChEBI:CHEBI:16810"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT   VARIANT         11
FT                   /note="H -> P (in strain: cv. Ehimehadaka No.1)"
FT   VARIANT         117
FT                   /note="K -> R (in strain: cv. Igri)"
FT   VARIANT         174
FT                   /note="Q -> R (in strain: cv. Ehimehadaka No.1 and cv.
FT                   Igri)"
FT   VARIANT         261
FT                   /note="K -> R (in strain: cv. Igri)"
FT   VARIANT         272
FT                   /note="A -> V (in strain: cv. Igri)"
FT   VARIANT         299
FT                   /note="K -> E (in strain: cv. Igri)"
FT   VARIANT         308
FT                   /note="T -> I (in strain: cv. Igri)"
SQ   SEQUENCE   339 AA;  37732 MW;  C5A315F018A6A03F CRC64;
     MENILHATPA HVSLPESFVF ASDKVPPATK AVVSLPIIDL SCGRDEVRRS ILEAGKELGF
     FQVVNHGVSK QVMRDMEGMC EQFFHLPAAD KASLYSEERH KPNRLFSGAT YDTGGEKYWR
     DCLRLACPFP VDDSINEWPD TPKGLRDVIE KFTSQTRDVG KELLRLLCEG MGIQADYFEG
     DLSGGNVILN INHYPSCPNP DKALGQPPHC DRNLITLLLP GAVNGLEVSY KGDWIKVDPA
     PNAFVVNFGQ QLEVVTNGLL KSIEHRAMTN SALARTSVAT FIMPTQECLI GPAKEFLSKE
     NPPCYRTTMF RDFMRIYNVV KLGSSLNLTT NLKNVQKEI
 
 
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