IDTB_CLAPA
ID IDTB_CLAPA Reviewed; 239 AA.
AC J7FIJ6;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 10.
DE RecName: Full=Terpene cyclase idtB {ECO:0000303|PubMed:23468653};
DE EC=4.2.3.- {ECO:0000305|PubMed:29457197};
DE AltName: Full=Indole-diterpene biosynthesis cluster protein B {ECO:0000303|PubMed:23468653};
GN Name=idtB {ECO:0000303|PubMed:23468653};
OS Claviceps paspali (Rye ergot fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=40601;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, AND FUNCTION.
RC STRAIN=RRC-1481;
RX PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA Yoshida R., Zeng Z.;
RT "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT Clavicipitaceae reveals dynamics of alkaloid loci.";
RL PLoS Genet. 9:E1003323-E1003323(2013).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=29457197; DOI=10.1007/s00253-018-8807-x;
RA Kozak L., Szilagyi Z., Vago B., Kakuk A., Toth L., Molnar I., Pocsi I.;
RT "Inactivation of the indole-diterpene biosynthetic gene cluster of
RT Claviceps paspali by Agrobacterium-mediated gene replacement.";
RL Appl. Microbiol. Biotechnol. 102:3255-3266(2018).
RN [3]
RP FUNCTION.
RX PubMed=32077051; DOI=10.1007/s12223-020-00777-6;
RA Kozak L., Szilagyi Z., Toth L., Pocsi I., Molnar I.;
RT "Functional characterization of the idtF and idtP genes in the Claviceps
RT paspali indole diterpene biosynthetic gene cluster.";
RL Folia Microbiol. (Praha) 65:605-613(2020).
CC -!- FUNCTION: Terpene cyclase; part of the gene cluster that mediates the
CC biosynthesis of paspalitrems, indole-diterpene (IDT) mycotoxins that
CC are potent tremorgens in mammals (PubMed:23468653, PubMed:29457197,
CC PubMed:32077051). The geranylgeranyl diphosphate (GGPP) synthase idtG
CC is proposed to catalyze the first step in IDT biosynthesis via
CC catalysis of a series of iterative condensations of isopentenyl
CC diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), geranyl
CC diphosphate (GPP), and farnesyl diphosphate (FPP), to form GGPP
CC (Probable). Condensation of indole-3-glycerol phosphate with GGPP by
CC the prenyltransferase idtC then forms 3-geranylgeranylindole (3-GGI)
CC (Probable). Epoxidation of the two terminal alkenes of the
CC geranylgeranyl moiety by the FAD-dependent monooxygenase idtM, and
CC cyclization by the terpene cyclase idtB then leads to the production of
CC paspaline (Probable). The cytochrome P450 monooxygenase idtP then
CC catalyzes oxidative elimination of the pendant methyl group at C-12 of
CC paspaline and generates the C-10 ketone to yield 13-desoxypaxilline
CC (PubMed:32077051). The cytochrome P450 monooxygenase idtQ may catalyze
CC the C-13 oxidation of 13-desoxypaxilline to afford paxilline
CC (Probable). Considering that both paspalicine and paxilline were
CC detected in C.paspali, idtQ also catalyzes the formation of paspalinine
CC from 13-desoxypaxilline via paspalicine as an intermediate (Probable).
CC Finally, the alpha-prenyltransferase idtF prenylates paspalinine at the
CC C-20 or the C-21 positions to yield paspalitrems A and C, respectively
CC (PubMed:32077051). The hydroxylation of paspalitrem A at C-32 by a
CC still unknown oxidase affords paspalitrem B (Probable).
CC {ECO:0000269|PubMed:23468653, ECO:0000269|PubMed:29457197,
CC ECO:0000269|PubMed:32077051, ECO:0000305|PubMed:29457197}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:29457197}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Simultaneous disruption of idtB, idtC, idtF and
CC idtG eliminates the biosynthesis of the whole spectrum of indole-
CC diterpenes reported to be produced by C.paspali, including paspaline,
CC paxilline, paspalinine, paspalitrem A and paspalitrem B.
CC {ECO:0000269|PubMed:29457197}.
CC -!- SIMILARITY: Belongs to the paxB family. {ECO:0000305}.
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DR EMBL; JN613321; AFO85421.1; -; Genomic_DNA.
DR AlphaFoldDB; J7FIJ6; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR039020; PaxB-like.
DR PANTHER; PTHR42038; PTHR42038; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Lyase; Membrane; Transmembrane; Transmembrane helix.
FT CHAIN 1..239
FT /note="Terpene cyclase idtB"
FT /id="PRO_0000451432"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 164
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 239 AA; 26633 MW; 39330615C16D2A93 CRC64;
MDGFSILHEP PAAYKEVKWM ADTFVAGMGL GWIVNYALMI RFSWKGRPHC MALLPLCNNI
AWELTYTIVY PSANRVELLV FAIGLTLNFF IMVGARRSAR VEWRHSPLLS EHAGFILLVG
TLLCFTGHVA LAMEIGPGLA YSWGAVVCQL ALSIGGLFQL LQRNSTAGTS WTLWSSRFLG
SCCTVAFAGL RCKYWPEVFG WLASPLVLWS LVTFLLADSA YGFCLYRVSH AETKARKKH
