IE1_NPVAC
ID IE1_NPVAC Reviewed; 582 AA.
AC P11138; Q9WIA6;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 3.
DT 23-FEB-2022, entry version 84.
DE RecName: Full=Trans-activating transcriptional regulatory protein;
DE AltName: Full=Immediate early protein 1;
DE Short=IE-1;
GN Name=IE1;
OS Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX NCBI_TaxID=46015;
OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3043024; DOI=10.1128/jvi.62.9.3193-3200.1988;
RA Chisholm G.E., Henner D.J.;
RT "Multiple early transcripts and splicing of the Autographa californica
RT nuclear polyhedrosis virus IE-1 gene.";
RL J. Virol. 62:3193-3200(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=E2;
RX PubMed=16789264; DOI=10.1128/jvi.61.7.2091-2099.1987;
RA Guarino L.A., Summers M.D.;
RT "Nucleotide sequence and temporal expression of a baculovirus regulatory
RT gene.";
RL J. Virol. 61:2091-2099(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT virus.";
RL Virology 202:586-605(1994).
RN [4]
RP FUNCTION.
RX PubMed=10936100; DOI=10.1006/viro.2000.0452;
RA Leisy D.J., Rohrmann G.F.;
RT "The Autographa californica nucleopolyhedrovirus IE-1 protein complex has
RT two modes of specific DNA binding.";
RL Virology 274:196-202(2000).
RN [5]
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=12186932; DOI=10.1128/jvi.76.18.9505-9515.2002;
RA Olson V.A., Wetter J.A., Friesen P.D.;
RT "Baculovirus transregulator IE1 requires a dimeric nuclear localization
RT element for nuclear import and promoter activation.";
RL J. Virol. 76:9505-9515(2002).
RN [6]
RP INTERACTION WITH LEF-3 AND HELI.
RX PubMed=15518813; DOI=10.1016/j.virol.2004.08.035;
RA Ito E., Sahri D., Knippers R., Carstens E.B.;
RT "Baculovirus proteins IE-1, LEF-3, and P143 interact with DNA in vivo: a
RT formaldehyde cross-linking study.";
RL Virology 329:337-347(2004).
RN [7]
RP FUNCTION.
RX PubMed=20705310; DOI=10.1016/j.virol.2010.07.020;
RA Efrose R., Swevers L., Iatrou K.;
RT "Baculoviruses deficient in ie1 gene function abrogate viral gene
RT expression in transduced mammalian cells.";
RL Virology 406:293-301(2010).
RN [8]
RP REGION, FUNCTION, PHOSPHORYLATION, AND MUTAGENESIS OF THR-15.
RX PubMed=22496221; DOI=10.1128/jvi.00373-12;
RA Taggart D.J., Mitchell J.K., Friesen P.D.;
RT "A conserved N-terminal domain mediates required DNA replication activities
RT and phosphorylation of the transcriptional activator IE1 of Autographa
RT californica multicapsid nucleopolyhedrovirus.";
RL J. Virol. 86:6575-6585(2012).
CC -!- FUNCTION: Regulatory transcriptional protein, which trans-activates
CC gene expression from early baculovirus promoters. Can also trans-
CC activate its own promoter, suggesting an autoregulation during
CC infection of host cells. Promotes also viral DNA genome replication via
CC the N-terminal region. {ECO:0000269|PubMed:10936100,
CC ECO:0000269|PubMed:20705310, ECO:0000269|PubMed:22496221}.
CC -!- SUBUNIT: Homodimer. Interacts with helicase and LEF-3.
CC {ECO:0000269|PubMed:12186932}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:12186932}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=The spliced form of IE-1 was expressed only in the first 6
CC hours postinfection, whereas the nonspliced IE-1 transcript was
CC expressed at constant level up to 24 hours.;
CC Name=Short;
CC IsoId=P11138-1; Sequence=Displayed;
CC Name=Long;
CC IsoId=P11138-2; Sequence=Not described;
CC -!- DOMAIN: The N-terminal region is required for origin-specific DNA
CC replication and phosphorylation. {ECO:0000269|PubMed:22496221}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:22496221}.
CC -!- MISCELLANEOUS: [Isoform Long]: Contains 54 additional residues in the
CC N-terminus. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nucleopolyhedrovirus IE-1 protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD18158.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M21884; AAA46696.1; -; Genomic_DNA.
DR EMBL; M16820; AAA46699.1; -; Genomic_DNA.
DR EMBL; L22858; AAA66777.1; -; Genomic_DNA.
DR EMBL; L22858; AAD18158.1; ALT_INIT; Genomic_DNA.
DR PIR; A27838; RGNVBV.
DR PIR; A28874; RGNVE2.
DR PIR; E72868; E72868.
DR PIR; F72867; F72867.
DR RefSeq; NP_054171.1; NC_001623.1.
DR RefSeq; NP_054178.1; NC_001623.1. [P11138-1]
DR GeneID; 1403980; -.
DR GeneID; 1403988; -.
DR KEGG; vg:1403980; -.
DR KEGG; vg:1403988; -.
DR Proteomes; UP000008292; Genome.
DR GO; GO:0039715; C:nuclear viral factory; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR GO; GO:0019079; P:viral genome replication; IDA:UniProtKB.
DR InterPro; IPR005092; TATR.
DR Pfam; PF03430; TATR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA replication; DNA-binding; Early protein;
KW Host nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Viral DNA replication.
FT CHAIN 1..582
FT /note="Trans-activating transcriptional regulatory protein"
FT /id="PRO_0000132847"
FT REGION 101..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 15
FT /note="T->A: Complete loss of replicative function."
FT /evidence="ECO:0000269|PubMed:22496221"
FT CONFLICT 60
FT /note="A -> R (in Ref. 2; AAA46699)"
FT /evidence="ECO:0000305"
FT CONFLICT 210..215
FT /note="NSNRFS -> QFQQVL (in Ref. 2; AAA46699)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="D -> G (in Ref. 1; AAA46696)"
FT /evidence="ECO:0000305"
FT CONFLICT 581..582
FT /note="FN -> L (in Ref. 2; AAA46699)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 582 AA; 66881 MW; 7515BD8991818580 CRC64;
MTQINFNASY TSASTPSRAS FDNSYSEFCD KQPNDYLSYY NHPTPDGADT VISDSETAAA
SNFLASVNSL TDNDLVECLL KTTDNLEEAV SSAYYSESLE QPVVEQPSPS SAYHAESFEH
SAGVNQPSAT GTKRKLDEYL DNSQGVVGQF NKIKLRPKYK KSTIQSCATL EQTINHNTNI
CTVASTQEIT HYFTNDFAPY LMRFDDNDYN SNRFSDHMSE TGYYMFVVKK SEVKPFEIIF
AKYVSNVVYE YTNNYYMVDN RVFVVTFDKI RFMISYNLVK ETGIEIPHSQ DVCNDETAAQ
NCKKCHFVDV HHTFKAALTS YFNLDMYYAQ TTFVTLLQSL GERKCGFLLS KLYEMYQDKN
LFTLPIMLSR KESNEIETAS NNFFVSPYVS QILKYSESVQ FPDNPPNKYV VDNLNLIVNK
KSTLTYKYSS VANLLFNNYK YHDNIASNNN AENLKKVKKE DGSMHIVEQY LTQNVDNVKG
HNFIVLSFKN EERLTIAKKN KEFYWISGEI KDVDVSQVIQ KYNRFKHHMF VIGKVNRRES
TTLHNNLLKL LALILQGLVP LSDAITFAEQ KLNCKYKKFE FN
