ID   IE247_HCMVT             Reviewed;         410 AA.
AC   P06435;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 2.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=45 kDa immediate-early protein 2;
DE            Short=IE2;
GN   Name=UL122;
OS   Human cytomegalovirus (strain Towne) (HHV-5) (Human herpesvirus 5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX   NCBI_TaxID=10363;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 45 KDA IMMEDIATE-EARLY PROTEIN
RP   2).
RX   PubMed=2542583; DOI=10.1128/jvi.63.6.2699-2708.1989;
RA   Stenberg R.M., Depto A.S., Fortney J., Nelson J.A.;
RT   "Regulated expression of early and late RNAs and proteins from the human
RT   cytomegalovirus immediate-early gene region.";
RL   J. Virol. 63:2699-2708(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 27 KDA IMMEDIATE-EARLY PROTEIN
RP   2).
RX   PubMed=2999423; DOI=10.1128/jvi.56.3.665-675.1985;
RA   Stenberg R.M., Witte P.R., Stinski M.F.;
RT   "Multiple spliced and unspliced transcripts from human cytomegalovirus
RT   immediate-early region 2 and evidence for a common initiation site within
RT   immediate-early region 1.";
RL   J. Virol. 56:665-675(1985).
CC   -!- FUNCTION: Activates the E1.7 promoter. This activation is augmented by
CC       the IE1 protein. It down-regulates the transcription of genes under the
CC       control of the major IE promoter.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=At least 2 isoforms are produced.;
CC       Name=45 kDa immediate-early protein 2;
CC         IsoId=P06435-1; Sequence=Displayed;
CC       Name=27 kDa immediate-early protein 2;
CC         IsoId=P06435-2; Sequence=VSP_015365;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA57205.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAA57206.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAA57207.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M26973; AAA57208.1; -; Genomic_DNA.
DR   EMBL; M26973; AAA57205.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M26972; AAA57205.1; JOINED; Genomic_DNA.
DR   EMBL; M26973; AAA57206.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M26972; AAA57206.1; JOINED; Genomic_DNA.
DR   EMBL; M26973; AAA57207.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M26972; AAA57207.1; JOINED; Genomic_DNA.
DR   EMBL; M11298; AAA45950.1; -; Genomic_DNA.
DR   PIR; A33347; EDBE4T.
DR   SMR; P06435; -.
DR   DrugBank; DB06759; Fomivirsen.
DR   PRIDE; P06435; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR005028; Herpes_IE2_3.
DR   Pfam; PF03361; Herpes_IE2_3; 1.
PE   4: Predicted;
KW   Activator; Alternative splicing; DNA-binding; Early protein;
KW   G0/G1 host cell cycle checkpoint dysregulation by virus;
KW   G1/S host cell cycle checkpoint dysregulation by virus;
KW   Host-virus interaction; Metal-binding;
KW   Modulation of host cell cycle by virus; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..410
FT                   /note="45 kDa immediate-early protein 2"
FT                   /id="PRO_0000115352"
FT   ZN_FING         257..283
FT                   /evidence="ECO:0000255"
FT   REGION          36..166
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..71
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        86..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        136..151
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         187..351
FT                   /note="Missing (in isoform 27 kDa immediate-early protein
FT                   2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_015365"
SQ   SEQUENCE   410 AA;  44774 MW;  F4B224A4502634F1 CRC64;
     MLPLIKQEDI KPEPDFTIQY RNKIIDTAGC IVISDSEEEQ GEEVETRGAT ASSPSTGSGT
     PRVTSPTHPL SQMNHPPLPD PLGRPDEDSS SSSSSCSSAS DSESESEEMK CSSGGGASVT
     SSHHGRGGFG GAASSSLLSC GHQSSGGAST GPRKKKSKRI SELDNEKVRN IMKDKNTPFC
     TPNVQTRRGR VKIDEVSRMF RNTNRSLEYK NLPFTIPSMH QVLDEAIKAC KTMQVNNKGI
     QIIYTRNHEV KSEVDAVRCR LGTMCNLALS TPFLMEHTMP VTHPPKVAQR TADACNEGVK
     AAWSLKELHT HQLCPRSSDY RNMIIHAATP VDLLGALNLC LPLMQKFPKQ VMVRIFSTNQ
     GGFMLPIYET ATKAYAVGQF EQPTETPPED LDTLSLAIEA AIQDLRNKSQ