IE2_HHV6U
ID IE2_HHV6U Reviewed; 1500 AA.
AC Q77Z83; O42080; Q69471; Q69564;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Immediate-early protein 2;
DE Short=IE2;
GN Name=U90/U87/U86; Synonyms=BCLF0;
OS Human herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B
OS lymphotropic virus).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Roseolovirus.
OX NCBI_TaxID=10370;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=7747482; DOI=10.1006/viro.1995.1228;
RA Gompels U.A., Nicholas J., Lawrence G.L., Jones M., Thomson B.J.,
RA Martin M.E.D., Efstathiou S., Craxton M.A., Macaulay H.A.;
RT "The DNA sequence of human herpesvirus-6: structure, coding content, and
RT genome evolution.";
RL Virology 209:29-51(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Soergel A., Schiewe U., Fleckenstein B., Neipel F.;
RT "Identification of an human herpesvirus-6 transcription unit homologous to
RT the immediate-early 2 gene of human cytomegalovirus -- evidence for an
RT early/late gene.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-1500.
RX PubMed=7941342; DOI=10.1006/viro.1994.1589;
RA Nicholas J.;
RT "Nucleotide sequence analysis of a 21-kbp region of the genome of human
RT herpesvirus-6 containing homologues of human cytomegalovirus major
RT immediate-early and replication genes.";
RL Virology 204:738-750(1994).
RN [4]
RP ALTERNATIVE SPLICING, AND SUBCELLULAR LOCATION.
RX PubMed=12367753; DOI=10.1016/s0168-1702(02)00126-0;
RA Papanikolaou E., Kouvatsis V., Dimitriadis G., Inoue N., Arsenakis M.;
RT "Identification and characterization of the gene products of open reading
RT frame U86/87 of human herpesvirus 6.";
RL Virus Res. 89:89-101(2002).
CC -!- FUNCTION: Transcriptional transactivator. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with human UBE2I in the nucleus. Although this
CC interaction does not promote IE2 sumoylation, it represses
CC transactivation activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:12367753}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=IE2 protein, Protein U90-U86/87;
CC IsoId=Q77Z83-1; Sequence=Displayed;
CC Name=2; Synonyms=IE1 protein, Protein U90-U89;
CC IsoId=Q69567-1; Sequence=External;
CC Name=3; Synonyms=IE-100;
CC IsoId=Q77Z83-2; Sequence=VSP_034763;
CC Name=4; Synonyms=IE-55;
CC IsoId=Q77Z83-3; Sequence=VSP_034764;
CC -!- DOMAIN: The C-terminus binds to a DNA fragment that contains the
CC transcription initiation site and an upstream sequence of the IEA
CC promoter.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA58335.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X83413; CAA58335.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF015297; AAB67722.1; -; mRNA.
DR EMBL; AF015298; AAB69858.1; -; Genomic_DNA.
DR EMBL; U13194; AAA68477.1; -; Genomic_DNA.
DR PIR; T03824; T03824.
DR RefSeq; NP_042979.2; NC_001664.2.
DR PDB; 5WX8; X-ray; 2.50 A; A/B/C/D=1324-1500.
DR PDBsum; 5WX8; -.
DR SMR; Q77Z83; -.
DR GeneID; 1487966; -.
DR KEGG; vg:1487966; -.
DR Proteomes; UP000009295; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR005028; Herpes_IE2_3.
DR InterPro; IPR005507; HHV6-IE.
DR Pfam; PF03361; Herpes_IE2_3; 1.
DR Pfam; PF03753; HHV6-IE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; DNA-binding; Host nucleus;
KW Host-virus interaction; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..1500
FT /note="Immediate-early protein 2"
FT /id="PRO_0000260225"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 516..979
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1024..1072
FT /note="Interaction with human UBE2I"
FT /evidence="ECO:0000250"
FT REGION 1041..1101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1149..1213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1225..1257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..234
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..364
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 433..471
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..555
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 561..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 606..626
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 633..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 687..709
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 710..859
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 860..877
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 878..979
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1057..1075
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1149..1186
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 170..1230
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_034764"
FT VAR_SEQ 614..1230
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_034763"
FT CONFLICT 136
FT /note="T -> S (in Ref. 1; CAA58335)"
FT /evidence="ECO:0000305"
FT CONFLICT 143..144
FT /note="RI -> L (in Ref. 1; CAA58335)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="H -> R (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 675
FT /note="D -> N (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 696
FT /note="E -> D (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 755..802
FT /note="Missing (in Ref. 1; CAA58335 and 3; AAA68477)"
FT /evidence="ECO:0000305"
FT CONFLICT 918
FT /note="E -> K (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 958
FT /note="R -> G (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 1169
FT /note="N -> D (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT CONFLICT 1430
FT /note="T -> S (in Ref. 2; AAB67722)"
FT /evidence="ECO:0000305"
FT HELIX 1335..1345
FT /evidence="ECO:0007829|PDB:5WX8"
FT STRAND 1352..1358
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1364..1376
FT /evidence="ECO:0007829|PDB:5WX8"
FT STRAND 1381..1384
FT /evidence="ECO:0007829|PDB:5WX8"
FT STRAND 1389..1391
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1399..1409
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1412..1419
FT /evidence="ECO:0007829|PDB:5WX8"
FT STRAND 1436..1444
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1445..1461
FT /evidence="ECO:0007829|PDB:5WX8"
FT TURN 1463..1465
FT /evidence="ECO:0007829|PDB:5WX8"
FT STRAND 1466..1474
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1481..1483
FT /evidence="ECO:0007829|PDB:5WX8"
FT HELIX 1484..1495
FT /evidence="ECO:0007829|PDB:5WX8"
SQ SEQUENCE 1500 AA; 168789 MW; 4EB4BBD1F25B39C0 CRC64;
MEPAKPSGNN MGSNDERMQD YRPDPMMEES IKEILEESLM CDTSFDDLII PGLESFGLII
PESSNNIESN NVEEGSDGEL KTLAEQKCKQ GNDNDVIQSA MKLSGLYCDA DITHTQPLSD
NTHQDPIYSQ ESRIFTKTIQ DPRIVAQTHR QCTSSASNLQ SNESGSTQVR FASELPNQLL
QPMYTSHNQN ANLQNNFTSL PYQPYHDPYR DIESSYRESR NTNRGYDYNF RHHPYRPRGG
NGKYNYYNPN SKYQQPYKRC FTRTYNRRGR GHRSYDCSDR SADLPYEHYT YPNYEQQNPD
PRMNNYKDFT QLTNKFNFES YDYSMAFSTD STHVQSDNYN HPTKAQTIPE TTKTKKHEAT
KDNETSTENQ VLTPDVISLS YRPSSYKMDI IKKIYDTDVI PLPKEALTAN GSNRDVDIQK
YKKAHIRCRS VQKKKERSSQ TNKHDENHAS SRSDLKERKS NENEDKAVTK ARDFSKLNPL
LSPLPLTPEP AIDFADHTDK FYSTPEFNQI QQNLHRSKTS LQDTVPISKH TPRAPTKDNS
YKKHHDSKDN YPKMKHSPGR TTSKKNTTNS NGHQNFKEVS VKNVSGKATS TSPKSKTHHY
SSSSDEEGQY KSPVKTIIQS PSPYCKLKNP SIMDKNSAKN HTASADKNLT DNSPIRSNLN
PTAFNKSNSN KSITDSTSNS DECTDRKPNC NSTKNESKDP NRTCGKNSDK HLSKSCTMAS
KRAPSRASSR ASSRDSSRAS SRASSRASSR DSSRASSRAS SRDSSRASSR ASSRASSKAS
SRASSRASSR ASSRDSSRAS SKASSRASSR DSSRASSRAS SRASSKASSR ASSRASSRAS
SRDSSRASSK ASSRASSRDS SRASSRDSSR DSSRASSRAS SRDSSRASSK ASRKASSRAS
SRASSRASSK ASGKASSEAS SRASSRNSSR ASSRASSRAS SRDSSRASSR ASSRDSSRAS
SKASRKASSR ASSRASSREL RQIYCDSNKR QTPPHDTSIN TKFEISEIKF RCGEDLNFYK
NTAARLQCFN HNDQFYNPRF RPHIRTNRKK SESTNDTDSE SSMSRCKSHC RNSPDSLTVV
RRKKHKSGSS SISSSIEENC RSNSHIVTGK EKFTPFYYQS SRTRSSSSSS SSSASLSCSK
STLKTCRKTQ YKDNKQIKSK SDSKHKTTNM SSDYESNRHA DVFKNSPEAG EKFPLHNSSP
FNTHEQSNHS ENAIDEEQKK APNITTSHLQ GKQNVRLHNT KKCKKKRPRD DDSDSSIKNF
CKKRISGAQK TESEVSEPDD LCYRDYVRLK ERKVSEKFKI HRGRVATKDF QKLFRNTMRA
FEYKQIPKKP CNEKNLKEAV YDICCNGLSN NAAIIMYFTR SKKVAQIIKI MQKELMIRPN
ITVSEAFKMN HAPPKYYDKD EIKRFIQLQK QGPQELWDKF ENNTTHDLFT RHSDVKTMII
YAATPIDFVG AVKTCNKYAK DNPKEIVLRV CSIIDGDNPI SIYNPISKEF KSKFSTLSKC
