ID   IELK1_BAURF             Reviewed;         144 AA.
AC   P84881;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Kunitz-type elastase inhibitor BrEI;
OS   Bauhinia rufa (Orchid tree) (Pauletia rufa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Cercidoideae; Cercideae; Bauhiniinae;
OC   Bauhinia.
OX   NCBI_TaxID=390785;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, GLYCOSYLATION AT ASN-38, AND DISULFIDE BOND.
RC   TISSUE=Seed {ECO:0000269|PubMed:16557451};
RX   PubMed=16557451; DOI=10.1055/s-2005-916237;
RA   Sumikawa J.T., Nakahata A.M., Fritz H., Mentele R., Sampaio M.U.,
RA   Oliva M.L.;
RT   "A Kunitz-type glycosylated elastase inhibitor with one disulfide bridge.";
RL   Planta Med. 72:393-397(2006).
CC   -!- FUNCTION: Inhibitor of porcine pancreatic elastase with a Ki of 27 nM.
CC       Does not inhibit human neutrophil elastase, bovine trypsin, human
CC       plasma kallikrein or porcine pancreatic kallikrein.
CC       {ECO:0000269|PubMed:16557451}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is:
CC       4.56, its MW is: 16992.79 kDa. {ECO:0000269|PubMed:16557451}.
CC   -!- SIMILARITY: Belongs to the leguminous Kunitz-type inhibitor family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P84881; -.
DR   SMR; P84881; -.
DR   iPTMnet; P84881; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Protease inhibitor; Serine protease inhibitor.
FT   CHAIN           1..144
FT                   /note="Kunitz-type elastase inhibitor BrEI"
FT                   /id="PRO_0000245796"
FT   SITE            66..67
FT                   /note="Reactive bond for elastase"
FT                   /evidence="ECO:0000269|PubMed:16557451"
FT   CARBOHYD        38
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16557451"
FT   DISULFID        41..88
FT                   /evidence="ECO:0000269|PubMed:16557451"
SQ   SEQUENCE   144 AA;  15823 MW;  A600B5356C75077F CRC64;
     ASPVLDANGD PLVPGGQYYV LPHIWPGPGG LSFEKTGNQT CPVSVFQLPR LPLEQNNGKP
     LVFTPVSETD DINEDTAVEI AFAEPPSCAE SGKWLIVNDF KEEYWSVGIG GPQDHEGYQT
     LTGYFKIHKV GSFAYMFSFL PFVR