IEND2_NEUCR
ID IEND2_NEUCR Reviewed; 452 AA.
AC Q35134; M1QL59;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 2.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Probable intron-encoded endonuclease 2;
DE EC=3.1.-.-;
GN ORFNames=NCU16009;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=3035337; DOI=10.1007/bf00333589;
RA Nelson M.A., Macino G.;
RT "Structure and expression of the overlapping ND4L and ND5 genes of
RT Neurospora crassa mitochondria.";
RL Mol. Gen. Genet. 206:307-317(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RA Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E.;
RT "Mitochondrial genetics of Neurospora.";
RL (In) Kueck U. (eds.);
RL The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112,
RL Springer-Verlag, Berlin-Heidelberg (2004).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Encoded from partially processed ndh-4L mRNA that
CC terminates with the in-frame coding sequence of the first intron.
CC -!- SIMILARITY: In the N-terminal section; belongs to the complex I subunit
CC 4L family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA28761.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X05115; CAA28761.1; ALT_SEQ; Genomic_DNA.
DR EMBL; KC683708; AGG15998.1; -; Genomic_DNA.
DR PIR; S10840; S10840.
DR RefSeq; YP_009126710.1; NC_026614.1.
DR AlphaFoldDB; Q35134; -.
DR SMR; Q35134; -.
DR EnsemblFungi; AGG15998; AGG15998; NCU16009.
DR GeneID; 23681562; -.
DR KEGG; ncr:NCU16009; -.
DR VEuPathDB; FungiDB:NCU16009; -.
DR InParanoid; Q35134; -.
DR Proteomes; UP000001805; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 2.
DR HAMAP; MF_01456; NDH1_NuoK; 1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR001133; NADH_UbQ_OxRdtase_chain4L/K.
DR InterPro; IPR039428; NUOK/Mnh_C1-like.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR Pfam; PF00420; Oxidored_q2; 1.
DR SUPFAM; SSF55608; SSF55608; 2.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..452
FT /note="Probable intron-encoded endonuclease 2"
FT /id="PRO_0000414734"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..80
FT /note="ndh-4L exon 1 encoded"
FT REGION 81..452
FT /note="ndh-4L intron 1 encoded"
SQ SEQUENCE 452 AA; 50902 MW; B572DB2A41AA5C1F CRC64;
MNITLILFLI GILGFVLNRK NIILMLISIE IMLLAITFLI LVSSLNMDDI IGQTYAIYII
VVAGAESAIG LAILVAFYRL INSPVKNPRS NYSGDPAPPS GGRGPYLHFN LLPVVSSCNL
IQSRNYSSVL HRKIPTHTSC VWAGLNPSFI TGFSDAEGSF VVTILKNPRY KIGWNVQARF
QIKLNEKDRA LLLLIQNYFD NIGYISKIND RSTVEFRVSD ITSLNNIIIP HFEKYQLITN
KYGDLVIFKQ IVSLMLENKH TTLEGLKEIL EHRASLNWGL SKTLKESFPS IIPVKRVKIE
NNILSNLSSL PLLPGGGNWV AGFSSGEANF FITMSGTKVW LRFSIAQDSR DILLLKSLVK
FFNCGYIAQY KNRKVCEFIV TKINDIIIYI IPFFDQYKIE GSKYNDYVKF KEAAILIKNK
EHLTEKGLNK IIELKNSLPP PASLEGGMNK NI
