IEND3_NEUCR
ID IEND3_NEUCR Reviewed; 533 AA.
AC Q35135; M1RFR6;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Probable intron-encoded endonuclease 3;
DE EC=3.1.-.-;
GN ORFNames=NCU16010;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=3035337; DOI=10.1007/bf00333589;
RA Nelson M.A., Macino G.;
RT "Structure and expression of the overlapping ND4L and ND5 genes of
RT Neurospora crassa mitochondria.";
RL Mol. Gen. Genet. 206:307-317(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RA Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E.;
RT "Mitochondrial genetics of Neurospora.";
RL (In) Kueck U. (eds.);
RL The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112,
RL Springer-Verlag, Berlin-Heidelberg (2004).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Encoded from partially processed ndh-5 mRNA that
CC terminates with the in-frame coding sequence of the first intron.
CC -!- SIMILARITY: In the N-terminal section; belongs to the complex I subunit
CC 5 family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA28764.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X05115; CAA28764.1; ALT_SEQ; Genomic_DNA.
DR EMBL; KC683708; AGG16001.1; -; Genomic_DNA.
DR PIR; S10841; S10841.
DR RefSeq; YP_009126713.1; NC_026614.1.
DR AlphaFoldDB; Q35135; -.
DR SMR; Q35135; -.
DR PRIDE; Q35135; -.
DR EnsemblFungi; AGG16001; AGG16001; NCU16010.
DR GeneID; 23681565; -.
DR KEGG; ncr:NCU16010; -.
DR VEuPathDB; FungiDB:NCU16010; -.
DR InParanoid; Q35135; -.
DR Proteomes; UP000001805; Mitochondrion.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR001516; Proton_antipo_N.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR SUPFAM; SSF55608; SSF55608; 2.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..533
FT /note="Probable intron-encoded endonuclease 3"
FT /id="PRO_0000414735"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..108
FT /note="ndh-5 exon 1 encoded"
FT REGION 109..533
FT /note="ndh-5 intron 1 encoded"
FT CONFLICT 385
FT /note="F -> L (in Ref. 1; CAA28764)"
FT /evidence="ECO:0000305"
FT CONFLICT 445
FT /note="D -> N (in Ref. 1; CAA28764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 533 AA; 60490 MW; D1EF09E1F7880F0B CRC64;
MYLSIIILPL LGSIVAGFFG RKVGVSGAQL ITCLSVIITT GLAILAFFEV GFNNIPVTIN
LFRWIDSEWY NILWGFQFDS LTVAMLIPVL IISSLVHIYS ISYMSHDPRG RVRGKRVYGD
KLSNSGEVLK LKVPSCSWKT MSGWSNYSGT VTSLKMSENK MDNRGSKSVV IDSNSTVKEQ
RVDGSWSIKS HLMDLRCTLR GFERNRGIKL GFNLQQGWNS AKIPSKQFDF KKKFSTYNST
LRVNPWVWSG LIDGEGSFNI IVDRNKSRKL GWRAQLKFQL SLHTKDLNLL YLLQQYLGGI
GSIHLARNRD IVNYSIDSIE DLNKLIIHLE NYPLLTQKAA DFFLFKQAVK LVNNKAHLTV
EGLNQIVNIK ASMNLGLSDT LKSEFAGYTP VERPVINCDN VFLDPYWISG FVSAEGNFDV
RMPSTNSKLG YRVQLRFRIS TLRVDIRLME KIVEYFGSGK IYKYGGKSAV SLTIVDFTDI
TNILVPFFNK YPIIGIKLYD YLDWCKIHSL MINRSHLTVE GINSISLLLG RRR
