ID   IEND4_NEUCR             Reviewed;         545 AA.
AC   Q35136; M1RV26;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Probable intron-encoded endonuclease 4;
DE            EC=3.1.-.-;
GN   ORFNames=NCU16011;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=3035337; DOI=10.1007/bf00333589;
RA   Nelson M.A., Macino G.;
RT   "Structure and expression of the overlapping ND4L and ND5 genes of
RT   Neurospora crassa mitochondria.";
RL   Mol. Gen. Genet. 206:307-317(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RA   Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E.;
RT   "Mitochondrial genetics of Neurospora.";
RL   (In) Kueck U. (eds.);
RL   The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112,
RL   Springer-Verlag, Berlin-Heidelberg (2004).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Encoded from partially processed ndh-5 mRNA that
CC       terminates with the in-frame coding sequence of the second intron.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the complex I subunit
CC       5 family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC       endonuclease family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA28766.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; X05115; CAA28766.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; KC683708; AGG16000.1; -; Genomic_DNA.
DR   RefSeq; YP_009126712.1; NC_026614.1.
DR   AlphaFoldDB; Q35136; -.
DR   SMR; Q35136; -.
DR   STRING; 367110.Q35136; -.
DR   EnsemblFungi; AGG16000; AGG16000; NCU16011.
DR   GeneID; 23681564; -.
DR   KEGG; ncr:NCU16011; -.
DR   VEuPathDB; FungiDB:NCU16011; -.
DR   InParanoid; Q35136; -.
DR   Proteomes; UP000001805; Mitochondrion.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   InterPro; IPR003945; NU5C-like.
DR   InterPro; IPR001516; Proton_antipo_N.
DR   PANTHER; PTHR42829; PTHR42829; 1.
DR   Pfam; PF00961; LAGLIDADG_1; 2.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   Pfam; PF00662; Proton_antipo_N; 1.
DR   SUPFAM; SSF55608; SSF55608; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..545
FT                   /note="Probable intron-encoded endonuclease 4"
FT                   /id="PRO_0000414736"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..239
FT                   /note="ndh-5 exons 1 and 2 encoded"
FT   REGION          240..545
FT                   /note="ndh-5 intron 2 encoded"
FT   CONFLICT        357
FT                   /note="H -> R (in Ref. 1; CAA28766)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        404
FT                   /note="H -> R (in Ref. 1; CAA28766)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        459
FT                   /note="E -> K (in Ref. 1; CAA28766)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        532
FT                   /note="M -> T (in Ref. 1; CAA28766)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   545 AA;  62419 MW;  2484D7FB25536198 CRC64;
     MYLSIIILPL LGSIVAGFFG RKVGVSGAQL ITCLSVIITT GLAILAFFEV GFNNIPVTIN
     LFRWIDSEWY NILWGFQFDS LTVAMLIPVL IISSLVHIYS ISYMSHDPHN QRFFSYLSLF
     TFMMIILVTA NNYLLMFVGW EGVGVCSYLL VSFWFTRIAA NQSSMSAFLT NRVGDCFLTI
     GMFVVLWTLG NLDYATVFSL APYINSDIAT IIGICLLIGA MAKSSQVGLH VWLPMAMEGF
     FSRAFLKLHY MQEHPVLSLG PLRFSLFGKI QDQGQFAGNS IRSSSEITSE AFMLKESWFK
     WWFIGFVEGD GSFIINKDGY LEFRITQSSP DAQILFMIKK ELGFGVVRKQ DSVRNTHCYR
     VRDKNNLIKL ISIFNGNIFL DTRKEQFKLW LNAFNLKYKE NIPHIDSSFR PNLDNAWLSG
     FTDAEGCFTC SVYDNKSNTA KLVRLRYILS QKGNSSCMEY LAEILGGKKH LLKSYEGYNV
     TVNTTKLSPI VQYFNLYPLK TKKYITYFNW IKIYKLVIDK KHNDPENLLL IMKYKNNINK
     SDYNK