ID   IER2_DANRE              Reviewed;         180 AA.
AC   B7SXM5;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Immediate early response gene 2 protein;
GN   Name=ier2 {ECO:0000312|ZFIN:ZDB-GENE-030131-9126};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000312|EMBL:ACJ01791.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR
RP   LOCATION, INTERACTION WITH FIBPB, AND DISRUPTION PHENOTYPE.
RX   PubMed=19164561; DOI=10.1073/pnas.0812880106;
RA   Hong S.K., Dawid I.B.;
RT   "FGF-dependent left-right asymmetry patterning in zebrafish is mediated by
RT   Ier2 and Fibp1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:2230-2235(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
CC   -!- FUNCTION: DNA-binding protein that seems to act as a transcription
CC       factor (By similarity). Mediates with FIBPB FGF-signaling in Kupffer's
CC       vesicle ciliogenesis and in the establishment of laterality in the
CC       embryo (PubMed:19164561). {ECO:0000250|UniProtKB:Q9BTL4,
CC       ECO:0000269|PubMed:19164561}.
CC   -!- SUBUNIT: Interacts with FIBPB. {ECO:0000269|PubMed:19164561}.
CC   -!- INTERACTION:
CC       B7SXM5; Q6T938: fibpb; NbExp=3; IntAct=EBI-15752758, EBI-15752740;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19164561}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q9BTL4}.
CC   -!- DEVELOPMENTAL STAGE: Expressed after the mid-blastula transition
CC       (PubMed:19164561). Expressed in Kupffer's vesicle (PubMed:19164561).
CC       {ECO:0000269|PubMed:19164561}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein causes severe
CC       deficits in laterality (PubMed:19164561). Partial loss of Kupffer's
CC       vesicle cilia with the length of the remaining cilia reduced
CC       (PubMed:19164561). Double morpholino knockdown of IER2 and FIBPB causes
CC       an almost complete loss of Kupffer's vesicle cilia (PubMed:19164561).
CC       {ECO:0000269|PubMed:19164561}.
CC   -!- SIMILARITY: Belongs to the IER family. {ECO:0000305}.
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DR   EMBL; EU815060; ACJ01791.1; -; mRNA.
DR   EMBL; CABZ01012976; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; B7SXM5; -.
DR   SMR; B7SXM5; -.
DR   DIP; DIP-48706N; -.
DR   IntAct; B7SXM5; 1.
DR   ZFIN; ZDB-GENE-030131-9126; ier2a.
DR   PhylomeDB; B7SXM5; -.
DR   PRO; PR:B7SXM5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IGI:ZFIN.
DR   GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR   GO; GO:0060026; P:convergent extension; IMP:ZFIN.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR   GO; GO:0070121; P:Kupffer's vesicle development; IMP:ZFIN.
DR   InterPro; IPR008653; IER.
DR   PANTHER; PTHR15895; PTHR15895; 1.
DR   Pfam; PF05760; IER; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..180
FT                   /note="Immediate early response gene 2 protein"
FT                   /id="PRO_0000433931"
FT   REGION          53..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        53..96
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   180 AA;  19684 MW;  BE11005D45B55706 CRC64;
     MDVTAEAKQI MVQALGKMYS SRSQRGGLRL HRSLLLTLVM KSARDIYHSA RLMSEKSGQS
     VTEECTSHTQ EPMDTSSSTA TPLRETSGQS SEDGQRSGLE GHPHPLNPAA DKENCGPSRP
     DRHSRKRRSK TATDSDFIPC KKAKLECAEV RGVLQNSSAN CGRALDSLSL VPMPRTIVTF