IER2_HUMAN
ID IER2_HUMAN Reviewed; 223 AA.
AC Q9BTL4; Q03827; Q2TAZ2;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Immediate early response gene 2 protein;
DE AltName: Full=Protein ETR101;
GN Name=IER2 {ECO:0000312|HGNC:HGNC:28871};
GN Synonyms=ETR101 {ECO:0000303|PubMed:19584537},
GN PIP92 {ECO:0000303|PubMed:22120713};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND VARIANT VAL-133.
RC TISSUE=Promyelocytic leukemia;
RX PubMed=2061303; DOI=10.1016/s0021-9258(18)98873-3;
RA Shimizu N., Ohta M., Fujiwara C., Sagara J., Mochizuki N., Oda T.,
RA Utlyama H.;
RT "Expression of a novel immediate early gene during 12-O-
RT tetradecanoylphorbol-13-acetate-induced macrophagic differentiation of HL-
RT 60 cells.";
RL J. Biol. Chem. 266:12157-12161(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, Duodenum, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=19584537; DOI=10.1271/bbb.90162;
RA Takaya T., Kasatani K., Noguchi S., Nikawa J.;
RT "Functional analyses of immediate early gene ETR101 expressed in yeast.";
RL Biosci. Biotechnol. Biochem. 73:1653-1660(2009).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=22120713; DOI=10.1038/onc.2011.535;
RA Neeb A., Wallbaum S., Novac N., Dukovic-Schulze S., Scholl I.,
RA Schreiber C., Schlag P., Moll J., Stein U., Sleeman J.P.;
RT "The immediate early gene Ier2 promotes tumor cell motility and metastasis,
RT and predicts poor survival of colorectal cancer patients.";
RL Oncogene 31:3796-3806(2012).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: DNA-binding protein that seems to act as a transcription
CC factor (PubMed:19584537). Involved in the regulation of neuronal
CC differentiation, acts upon JNK-signaling pathway activation and plays a
CC role in neurite outgrowth in hippocampal cells (By similarity). May
CC mediate with FIBP FGF-signaling in the establishment of laterality in
CC the embryo (By similarity). Promotes cell motility, seems to stimulate
CC tumor metastasis (PubMed:22120713). {ECO:0000250|UniProtKB:B7SXM5,
CC ECO:0000250|UniProtKB:Q6P7D3, ECO:0000269|PubMed:19584537,
CC ECO:0000269|PubMed:22120713}.
CC -!- INTERACTION:
CC Q9BTL4; O94817: ATG12; NbExp=3; IntAct=EBI-2806011, EBI-746742;
CC Q9BTL4; O75031: HSF2BP; NbExp=3; IntAct=EBI-2806011, EBI-7116203;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22120713}. Nucleus
CC {ECO:0000269|PubMed:22120713}. Note=Cytoplasmic during quiescence,
CC translocates to the nucleus upon stimulation.
CC {ECO:0000269|PubMed:22120713}.
CC -!- TISSUE SPECIFICITY: Expressed in activated T-cells (at protein level)
CC (PubMed:22120713). Expression increases in metastatic tumor cells (at
CC protein level) (PubMed:22120713). {ECO:0000269|PubMed:22120713}.
CC -!- INDUCTION: By growth factors and 12-O-tetradecanoylphorbol-13-acetate
CC (TPA). {ECO:0000269|PubMed:2061303}.
CC -!- SIMILARITY: Belongs to the IER family. {ECO:0000305}.
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DR EMBL; M62831; AAA35814.1; -; mRNA.
DR EMBL; CR450352; CAG29348.1; -; mRNA.
DR EMBL; BT019466; AAV38273.1; -; mRNA.
DR EMBL; BC003625; AAH03625.1; -; mRNA.
DR EMBL; BC072432; AAH72432.1; -; mRNA.
DR EMBL; BC110647; AAI10648.1; -; mRNA.
DR CCDS; CCDS12295.1; -.
DR PIR; A40866; A40866.
DR RefSeq; NP_004898.2; NM_004907.2.
DR AlphaFoldDB; Q9BTL4; -.
DR SMR; Q9BTL4; -.
DR BioGRID; 114959; 37.
DR IntAct; Q9BTL4; 21.
DR STRING; 9606.ENSP00000465617; -.
DR iPTMnet; Q9BTL4; -.
DR PhosphoSitePlus; Q9BTL4; -.
DR BioMuta; IER2; -.
DR DMDM; 73920026; -.
DR EPD; Q9BTL4; -.
DR jPOST; Q9BTL4; -.
DR MassIVE; Q9BTL4; -.
DR MaxQB; Q9BTL4; -.
DR PaxDb; Q9BTL4; -.
DR PeptideAtlas; Q9BTL4; -.
DR PRIDE; Q9BTL4; -.
DR ProteomicsDB; 78996; -.
DR Antibodypedia; 13536; 124 antibodies from 22 providers.
DR DNASU; 9592; -.
DR Ensembl; ENST00000292433.4; ENSP00000292433.2; ENSG00000160888.7.
DR Ensembl; ENST00000587885.1; ENSP00000467294.1; ENSG00000160888.7.
DR Ensembl; ENST00000588173.1; ENSP00000465617.1; ENSG00000160888.7.
DR GeneID; 9592; -.
DR KEGG; hsa:9592; -.
DR MANE-Select; ENST00000292433.4; ENSP00000292433.2; NM_004907.3; NP_004898.2.
DR UCSC; uc002mwr.4; human.
DR CTD; 9592; -.
DR DisGeNET; 9592; -.
DR GeneCards; IER2; -.
DR HGNC; HGNC:28871; IER2.
DR HPA; ENSG00000160888; Low tissue specificity.
DR neXtProt; NX_Q9BTL4; -.
DR OpenTargets; ENSG00000160888; -.
DR PharmGKB; PA134982230; -.
DR VEuPathDB; HostDB:ENSG00000160888; -.
DR eggNOG; ENOG502S19F; Eukaryota.
DR GeneTree; ENSGT00900000141021; -.
DR HOGENOM; CLU_1234685_0_0_1; -.
DR InParanoid; Q9BTL4; -.
DR OMA; MVMRSAR; -.
DR OrthoDB; 1503216at2759; -.
DR PhylomeDB; Q9BTL4; -.
DR PathwayCommons; Q9BTL4; -.
DR SignaLink; Q9BTL4; -.
DR BioGRID-ORCS; 9592; 27 hits in 1078 CRISPR screens.
DR ChiTaRS; IER2; human.
DR GenomeRNAi; 9592; -.
DR Pharos; Q9BTL4; Tbio.
DR PRO; PR:Q9BTL4; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9BTL4; protein.
DR Bgee; ENSG00000160888; Expressed in nipple and 210 other tissues.
DR ExpressionAtlas; Q9BTL4; baseline and differential.
DR Genevisible; Q9BTL4; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0048870; P:cell motility; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0071774; P:response to fibroblast growth factor; ISS:UniProtKB.
DR InterPro; IPR008653; IER.
DR PANTHER; PTHR15895; PTHR15895; 1.
DR Pfam; PF05760; IER; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..223
FT /note="Immediate early response gene 2 protein"
FT /id="PRO_0000190435"
FT REGION 63..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VARIANT 133
FT /note="A -> V (in dbSNP:rs1042164)"
FT /evidence="ECO:0000269|PubMed:2061303"
FT /id="VAR_057581"
FT CONFLICT 165
FT /note="A -> G (in Ref. 1; AAA35814)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 223 AA; 24196 MW; CDC40214CCE0C2EE CRC64;
MEVQKEAQRI MTLSVWKMYH SRMQRGGLRL HRSLQLSLVM RSARELYLSA KVEALEPEVS
LPAALPSDPR LHPPREAEST AETATPDGEH PFPEPMDTQE APTAEETSAC CAPRPAKVSR
KRRSSSLSDG GDAGLVPSKK ARLEEKEEEE GASSEVADRL QPPPAQAEGA FPNLARVLQR
RFSGLLNCSP AAPPTAPPAC EAKPACRPAD SMLNVLVRAV VAF