IER5_MOUSE
ID IER5_MOUSE Reviewed; 308 AA.
AC O89113; Q8CGI0;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Immediate early response gene 5 protein;
GN Name=Ier5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND INDUCTION.
RC STRAIN=129/Sv; TISSUE=Brain;
RX PubMed=10049588; DOI=10.1006/geno.1998.5679;
RA Williams M., Lyu M.-S., Yang Y.-L., Lin E.P., Dunbrack R., Birren B.,
RA Cunningham J., Hunter K.;
RT "Ier5, a novel member of the slow-kinetics immediate-early genes.";
RL Genomics 55:327-334(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N-3; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a role as a transcription factor. Mediates positive
CC transcriptional regulation of several chaperone gene during the heat
CC shock response in a HSF1-dependent manner. Mediates negative
CC transcriptional regulation of CDC25B expression. Plays a role in the
CC dephosphorylation of the heat shock factor HSF1 and ribosomal protein
CC S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the
CC regulation of cell proliferation and resistance to thermal stress.
CC Involved in the cell cycle checkpoint and survival in response to
CC ionizing radiation. Associates with chromatin to the CDC25B promoter.
CC {ECO:0000250|UniProtKB:Q5VY09}.
CC -!- SUBUNIT: Monomer. Homodimer. Associates with the catalytic subunit of
CC protein phosphatase PP2A. Interacts (via N- and C-terminal regions)
CC with PPP2R2B. Interacts with PPP2R2A, PPP2R2C and PPP2R2D. Interacts
CC (via N-terminus) with RPS6KB1. Interacts (via central region) with
CC HSF1; this interaction promotes PPP2CA-induced HSF1 dephosphorylation,
CC leading to enhanced HSF1 transcriptional activity.
CC {ECO:0000250|UniProtKB:Q5VY09}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5VY09}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q5VY09}. Note=Predominantly cytoplasmic.
CC Translocated in the nucleus during heat shock.
CC {ECO:0000250|UniProtKB:Q5VY09}.
CC -!- INDUCTION: By serum and growth factors. {ECO:0000269|PubMed:10049588}.
CC -!- SIMILARITY: Belongs to the IER family. {ECO:0000305}.
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DR EMBL; AF079527; AAC31197.1; -; mRNA.
DR EMBL; AF079528; AAC28389.1; -; Genomic_DNA.
DR EMBL; BC037147; AAH37147.1; -; mRNA.
DR EMBL; BC051986; AAH51986.1; -; mRNA.
DR CCDS; CCDS15382.1; -.
DR RefSeq; NP_034630.1; NM_010500.2.
DR AlphaFoldDB; O89113; -.
DR SMR; O89113; -.
DR BioGRID; 200519; 3.
DR IntAct; O89113; 1.
DR STRING; 10090.ENSMUSP00000056948; -.
DR PhosphoSitePlus; O89113; -.
DR EPD; O89113; -.
DR PaxDb; O89113; -.
DR PRIDE; O89113; -.
DR ProteomicsDB; 273092; -.
DR Antibodypedia; 34432; 160 antibodies from 22 providers.
DR Ensembl; ENSMUST00000055322; ENSMUSP00000056948; ENSMUSG00000056708.
DR GeneID; 15939; -.
DR KEGG; mmu:15939; -.
DR UCSC; uc007dax.2; mouse.
DR CTD; 51278; -.
DR MGI; MGI:1337072; Ier5.
DR VEuPathDB; HostDB:ENSMUSG00000056708; -.
DR eggNOG; ENOG502S0NB; Eukaryota.
DR GeneTree; ENSGT00900000141021; -.
DR HOGENOM; CLU_057338_0_0_1; -.
DR InParanoid; O89113; -.
DR OMA; PCGCPLG; -.
DR OrthoDB; 1503216at2759; -.
DR PhylomeDB; O89113; -.
DR TreeFam; TF331376; -.
DR BioGRID-ORCS; 15939; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Ier5; mouse.
DR PRO; PR:O89113; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; O89113; protein.
DR Bgee; ENSMUSG00000056708; Expressed in granulocyte and 233 other tissues.
DR ExpressionAtlas; O89113; baseline and differential.
DR Genevisible; O89113; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000159; C:protein phosphatase type 2A complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0034605; P:cellular response to heat; ISS:UniProtKB.
DR GO; GO:1900036; P:positive regulation of cellular response to heat; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISS:UniProtKB.
DR InterPro; IPR008653; IER.
DR PANTHER; PTHR15895; PTHR15895; 1.
DR Pfam; PF05760; IER; 2.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; Nucleus; Reference proteome; Repressor;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..308
FT /note="Immediate early response gene 5 protein"
FT /id="PRO_0000190439"
FT REGION 63..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..109
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 308 AA; 31784 MW; F9484E63F69FC02B CRC64;
MEFKLEAHRI VSISLGKIYN SRVQRGGIKL HKNLLVSLVL RSARQVYLSD PCPGLYLAGP
AGTPAVPPPQ QPGEPVAGPP SGWGEPPPPV ARAAWPEPEP QPQPQPQRPS VCHTPGAGSS
EPVAAVAGSG EALRGGEEDS AAAAWGRVER PRAASSGGGS DACPEGPRAV RRPCGCPPAV
EERSSEDGSP APPAPCPRKR GAAGVGGGRA SCSAPGSTPL KKPRRNSEEQ PVTGEDDTDE
EMETGNVANL ISIFGSSFSG LLRKSPAGGR EEEEAEEGGP EAAEPGQICC DKPVLRDMSP
WSTAIVAF
