APC9_YEAST
ID APC9_YEAST Reviewed; 265 AA.
AC Q12107; D6VYA2;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Anaphase-promoting complex subunit 9;
GN Name=APC9; OrderedLocusNames=YLR102C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, AND SUBUNIT.
RX PubMed=9469814; DOI=10.1126/science.279.5354.1216;
RA Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J.,
RA Mann M., Nasmyth K.;
RT "Mass spectrometric analysis of the anaphase-promoting complex from yeast:
RT identification of a subunit related to cullins.";
RL Science 279:1216-1219(1998).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C is thought to confer substrate specificity and, in the
CC presence of ubiquitin-conjugating E2 enzymes, it catalyzes the
CC formation of protein-ubiquitin conjugates that are subsequently
CC degraded by the 26S proteasome. In early mitosis, the APC/C is
CC activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5,
CC and other anaphase inhibitory proteins for proteolysis, thereby
CC triggering the separation of sister chromatids at the metaphase-to-
CC anaphase transition. In late mitosis and in G1, degradation of CLB5
CC allows activation of the APC/C by CDH1, which is needed to destroy
CC CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and
CC creating the low CDK state necessary for cytokinesis and for reforming
CC prereplicative complexes in G1 prior to another round of replication.
CC {ECO:0000269|PubMed:9469814}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 13 subunits that stay
CC tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5,
CC APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.
CC {ECO:0000269|PubMed:9469814}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
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DR EMBL; Z73274; CAA97667.1; -; Genomic_DNA.
DR EMBL; U53876; AAB67545.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09418.1; -; Genomic_DNA.
DR PIR; S64938; S64938.
DR RefSeq; NP_013203.1; NM_001181989.1.
DR AlphaFoldDB; Q12107; -.
DR BioGRID; 31375; 110.
DR ComplexPortal; CPX-756; Anaphase-Promoting core complex.
DR ComplexPortal; CPX-760; Anaphase-Promoting Complex, CDC20 variant.
DR ComplexPortal; CPX-761; Anaphase-Promoting Complex, CDH1 variant.
DR ComplexPortal; CPX-762; Anaphase-Promoting complex AMA1 variant.
DR DIP; DIP-1129N; -.
DR IntAct; Q12107; 20.
DR MINT; Q12107; -.
DR STRING; 4932.YLR102C; -.
DR PaxDb; Q12107; -.
DR PRIDE; Q12107; -.
DR EnsemblFungi; YLR102C_mRNA; YLR102C; YLR102C.
DR GeneID; 850792; -.
DR KEGG; sce:YLR102C; -.
DR SGD; S000004092; APC9.
DR VEuPathDB; FungiDB:YLR102C; -.
DR eggNOG; ENOG502S206; Eukaryota.
DR HOGENOM; CLU_077720_0_0_1; -.
DR InParanoid; Q12107; -.
DR OMA; CYEEDNC; -.
DR BioCyc; YEAST:G3O-32250-MON; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q12107; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12107; protein.
DR GO; GO:0005680; C:anaphase-promoting complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IDA:ComplexPortal.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0031497; P:chromatin assembly; IGI:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IDA:ComplexPortal.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR InterPro; IPR024274; APC9.
DR Pfam; PF12856; ANAPC9; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Mitosis; Nucleus; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..265
FT /note="Anaphase-promoting complex subunit 9"
FT /id="PRO_0000064626"
SQ SEQUENCE 265 AA; 30856 MW; 63423CC7CDFF47B2 CRC64;
MNQNGDKNEG KLFQLPSLPP WKTPRFNKAN FNNFTTPLRK RSTRVINDDS MPITGEVLEE
RTADDLYGIN MDVDEVDYLN TLSHIEEEKQ YDYSPFCERN TLRESRIDSF LKAERAAHCL
VFHKVGHLDG IDSYRPDIDI MCGEEANKYD SANPEGNGSM LLESVPGCNK EDLERLSRRE
FVTNSKPNMR RLDDIINHET NALKSFWNDS GLVNSLQSHH LHEEYLLLQE ELKNVYKIKC
HDRVPIESLR DKCRRHYSNE DSSFL
