IF122_CAEEL
ID IF122_CAEEL Reviewed; 1192 AA.
AC G5EFW7;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Intraflagellar transport protein 122 homolog {ECO:0000250|UniProtKB:Q9HBG6};
DE AltName: Full=Abnormal dauer formation protein 10 {ECO:0000312|WormBase:F23B2.4};
GN Name=daf-10 {ECO:0000312|WormBase:F23B2.4};
GN ORFNames=F23B2.4 {ECO:0000312|WormBase:F23B2.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:ABC88647.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16648645; DOI=10.1534/genetics.106.056721;
RA Bell L.R., Stone S., Yochem J., Shaw J.E., Herman R.K.;
RT "The molecular identities of the Caenorhabditis elegans intraflagellar
RT transport genes dyf-6, daf-10 and osm-1.";
RL Genetics 173:1275-1286(2006).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=7240452; DOI=10.1002/cne.901980305;
RA Albert P.S., Brown S.J., Riddle D.L.;
RT "Sensory control of dauer larva formation in Caenorhabditis elegans.";
RL J. Comp. Neurol. 198:435-451(1981).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=7705621; DOI=10.1093/genetics/139.1.171;
RA Starich T.A., Herman R.K., Kari C.K., Yeh W.H., Schackwitz W.S.,
RA Schuyler M.W., Collet J., Thomas J.H., Riddle D.L.;
RT "Mutations affecting the chemosensory neurons of Caenorhabditis elegans.";
RL Genetics 139:171-188(1995).
RN [5] {ECO:0000305}
RP SUBCELLULAR LOCATION.
RX PubMed=11301258; DOI=10.1016/s0960-9822(01)00122-1;
RA Qin H., Rosenbaum J.L., Barr M.M.;
RT "An autosomal recessive polycystic kidney disease gene homolog is involved
RT in intraflagellar transport in C. elegans ciliated sensory neurons.";
RL Curr. Biol. 11:457-461(2001).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=23284299; DOI=10.1371/journal.pgen.1003133;
RA Gaglia M.M., Jeong D.E., Ryu E.A., Lee D., Kenyon C., Lee S.J.;
RT "Genes that act downstream of sensory neurons to influence longevity, dauer
RT formation, and pathogen responses in Caenorhabditis elegans.";
RL PLoS Genet. 8:E1003133-E1003133(2012).
RN [7] {ECO:0000305}
RP FUNCTION.
RX PubMed=25830239; DOI=10.1371/journal.pgen.1005023;
RA Horikawa M., Sural S., Hsu A.L., Antebi A.;
RT "Co-chaperone p23 regulates C. elegans lifespan in response to
RT temperature.";
RL PLoS Genet. 11:E1005023-E1005023(2015).
RN [8]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX A, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Component of the IFT complex A (IFT-A), a complex required
CC for retrograde ciliary transport and entry into cilia of G protein-
CC coupled receptors (GPCRs) (PubMed:28479320). Plays a role in chemotaxis
CC and sensory perception (PubMed:7240452, PubMed:7705621). Required for
CC entry into and exit from the dauer larval stage and this may be
CC mediated by daf-12, daf-16 and daf-41 (PubMed:7240452, PubMed:23284299,
CC PubMed:25830239). Controls the behavioral response, namely the
CC avoidance response, and pathogen-responsive gene expression in the
CC response to pathogenic bacteria such as E.coli and P. aeruginosa
CC (PubMed:23284299). {ECO:0000269|PubMed:11301258,
CC ECO:0000269|PubMed:23284299, ECO:0000269|PubMed:25830239,
CC ECO:0000269|PubMed:28479320, ECO:0000269|PubMed:7240452,
CC ECO:0000269|PubMed:7705621}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) composed of at least
CC che-11, daf-10, dyf-2, ift-139, ift-43 and ifta-1.
CC {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000305|PubMed:11301258}. Note=Shuttles at the same rate as
CC intraflagellar transport (IFT) complex B proteins such as osm-1, osm-5
CC and osm-6 along the cilia of the amphid and phasmid sensory neurons.
CC {ECO:0000269|PubMed:11301258}.
CC -!- DISRUPTION PHENOTYPE: Sterile. Defective chemotaxis with 86% of mutants
CC exhibiting reduced tracking to the chemoattractant ammonium chloride.
CC {ECO:0000269|PubMed:7705621}.
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DR EMBL; DQ360810; ABC88647.1; -; mRNA.
DR EMBL; BX284604; CAB05177.4; -; Genomic_DNA.
DR PIR; H88772; H88772.
DR PIR; T21306; T21306.
DR RefSeq; NP_501591.2; NM_069190.3.
DR AlphaFoldDB; G5EFW7; -.
DR SMR; G5EFW7; -.
DR ComplexPortal; CPX-1289; Intraflagellar transport complex A.
DR STRING; 6239.F23B2.4; -.
DR PaxDb; G5EFW7; -.
DR EnsemblMetazoa; F23B2.4.1; F23B2.4.1; WBGene00000906.
DR GeneID; 184883; -.
DR KEGG; cel:CELE_F23B2.4; -.
DR CTD; 184883; -.
DR WormBase; F23B2.4; CE40504; WBGene00000906; daf-10.
DR eggNOG; KOG1538; Eukaryota.
DR GeneTree; ENSGT00390000001016; -.
DR HOGENOM; CLU_008896_0_0_1; -.
DR InParanoid; G5EFW7; -.
DR OMA; QSYTIGC; -.
DR OrthoDB; 449704at2759; -.
DR PhylomeDB; G5EFW7; -.
DR Reactome; R-CEL-5610787; Hedgehog 'off' state.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:G5EFW7; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00000906; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IC:ComplexPortal.
DR GO; GO:0030991; C:intraciliary transport particle A; ISS:UniProtKB.
DR GO; GO:0097730; C:non-motile cilium; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR GO; GO:0060271; P:cilium assembly; IGI:UniProtKB.
DR GO; GO:0035721; P:intraciliary retrograde transport; IBA:GO_Central.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:WormBase.
DR GO; GO:0061066; P:positive regulation of dauer larval development; IMP:WormBase.
DR GO; GO:0061512; P:protein localization to cilium; IBA:GO_Central.
DR GO; GO:0097500; P:receptor localization to non-motile cilium; IMP:WormBase.
DR GO; GO:0061065; P:regulation of dauer larval development; IGI:UniProtKB.
DR GO; GO:0046626; P:regulation of insulin receptor signaling pathway; IGI:WormBase.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR039857; Ift122.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR12764; PTHR12764; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell projection; Chemotaxis; Cilium; Reference proteome; Repeat; Transport;
KW WD repeat.
FT CHAIN 1..1192
FT /note="Intraflagellar transport protein 122 homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436568"
FT REPEAT 17..57
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 58..97
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 99..136
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 138..176
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 236..275
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 277..320
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 475..513
FT /note="WD 7"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1192 AA; 136068 MW; D15D251DD2088D27 CRC64;
MRPNLLWVDK ILDENNEAGV CIYDLAFKPD GSELLLAADN KVYLFDVNEG GQMQTLKGHK
DLVYTVAWSH NGELFASGGA DKLVILWNEK HEGTLRYSHT DVIQCMMFNP CNQILLTCAL
NEFGLWSTAD KNVIKQRSVV RCCSCAWNTD GTIFAIGHGD GTITLRKGTN ATEEPSIIIQ
RDNEPIWGIA FSSNRTFASR DSQGNPMGID EIMAVIDWNK TLSFYSLDGT FIESKNLEFE
PHCISYCLNG EYLLIGGSDK ILKIYTRKGV LLGTVAQMDH WIWSVTVRPN SQTVAMGCVD
GTIACYNLVF STVHCVDHAR YANRKSMTDV FVQNLEYRTS SNICCHDLVK KMSLYDTKLA
VQLSDKIQIY KQTGGVSKNE RRKQLKYTLQ DTIRKDLSFS LMVVTHGHLV VCNDEKLECY
DFKGIKKRSW NMKSIVRYLR VLGGPAHRET LVLGTTDGGV YKVFIDNDYP ILLDSRKTAI
KCIDINANRT VLASIEDTLV CKWSDIATGE TLLQEPGCYS VVFNTVNENL FAFTTNNMLH
VRTLAAPGHT TRGVGYVLGF VKNRTFCLVQ YNLIPLEVPY TIHLYQYIER GDFKEALRIA
CLGVVKNDWK YLANKALDAL EFDVARKAYK RVRDRKMLRM VWELKKMKSN GEPDAILRAT
ILAYTKKFRE AAKIFKENGF ENRAMELFTD MRMFDDVQEV MTTASGETKK MLMRKRASWA
RDANQPKIAA EMLISSGDLD KAALLIIDND WLELAIEISH KIDRSDLETM KKLSAYFIRK
HEFGLASRIF QSINDMKSIV DMHVNAGHWT DAFAIADRHP KYVEDVYLPY ARFLAERDRF
EEAQKAFHRA GKEQEAMHVL EQLTSNSVNE NRFADAGFYY WLLSQQYLDR SQTEENLTLL
NKAKEAASLA DAYYAYYPVF IFCSQPFSFE RNENILNMAR YLTFTPYIDN ISKVFVYFTI
AKIANEMGAY KSARTALDQL TNLRVLPQFE LDGQIEVMTL NIRAKPFTDV ESMQPMCYRC
GLNNPLLGGM SCIHCETPFI ISFVSFDILP LIEFKIENDI SFDEAKELIE SEPPLSDDDY
NPLRGLKKGI KEIILNRESL SKLEQGHVII QTFPPPLAPK FLFNVMPSIT IAQCKGCNKV
FDLDDFEMAC LRKGHCPFCR TSYDRNEAFF VDEEEDEDNT NIPSFGQFSR FS
