APCB_NEOYE
ID APCB_NEOYE Reviewed; 161 AA.
AC P59857; Q1XDM6;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Allophycocyanin beta chain;
GN Name=apcB;
OS Neopyropia yezoensis (Susabi-nori) (Pyropia yezoensis).
OG Plastid; Chloroplast.
OC Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Neopyropia.
OX NCBI_TaxID=2788;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=U-51;
RA Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T.,
RA Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
RT "Whole genome sequence of Porphyra yezoensis chloroplast.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP CRYSTALLIZATION.
RX PubMed=9761868; DOI=10.1107/s0907444997017824;
RA Liu J.-Y., Zhang J.-P., Wan Z.-L., Liang D.-C., Zhang J.-P., Wu H.-J.;
RT "Crystallization and preliminary X-ray studies of allophycocyanin from red
RT alga Porphyra yezoensis.";
RL Acta Crystallogr. D 54:662-664(1998).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH APCA AND
RP PHYCOCYANOBILIN, AND SUBUNIT.
RX PubMed=10358042; DOI=10.1074/jbc.274.24.16945;
RA Liu J.-Y., Jiang T., Zhang J.-P., Liang D.-C.;
RT "Crystal structure of allophycocyanin from red algae Porphyra yezoensis at
RT 2.2-A resolution.";
RL J. Biol. Chem. 274:16945-16952(1999).
CC -!- FUNCTION: Light-harvesting photosynthetic tetrapyrrole chromophore-
CC protein from the phycobiliprotein complex.
CC -!- SUBUNIT: Heterododecamer of 6 alpha and 6 beta chains. The basic
CC functional unit of phycobiliproteins is a ring-shaped hexamer formed
CC from two back-to-back trimers contacting via the alpha chain subunits.
CC The trimers are composed of alpha/beta subunit heterodimers arranged
CC around a three-fold axis of symmetry. The phycoerythrins also contain a
CC gamma subunit which is located in the center of the hexamer.
CC {ECO:0000269|PubMed:10358042}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane;
CC Peripheral membrane protein; Stromal side. Note=Forms the core of the
CC phycobilisome.
CC -!- PTM: Contains one covalently linked phycocyanobilin chromophore.
CC -!- MISCELLANEOUS: The light-harvesting antenna system in red algae and
CC cyanobacteria is formed of phycobilisomes. These are composed of the
CC phycobiliproteins phycoerythrin (CPE), phycocyanin (CPC) and
CC allophycocyanin (APC). Cyanobacteria also contain phycoerythrocyanin
CC (PCC). The phycobiliproteins all share the same subunit composition and
CC organization with variations in the covalently bound open-chain
CC tetrapyrrole chromophores. The phycobiliprotein complexes are arranged
CC sequentially in antenna complexes linked by linker proteins with CPE at
CC the periphery, CPC in the middle and APC at the core feeding to the
CC photosynthetic reaction center. Allophycocyanin has a maximum
CC absorption at approximately 650 nanometers.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
CC -!- CAUTION: Neither a protein sequence nor a nucleotide sequence has been
CC determined for this protein. This is the sequence of a model fit to
CC electron density plots at 2.2 Angstroms resolution. The N-
CC methylasparagine modification was omitted in the model. {ECO:0000305}.
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DR EMBL; AP006715; BAE92385.1; -; Genomic_DNA.
DR RefSeq; YP_536942.1; NC_007932.1.
DR PDB; 1KN1; X-ray; 2.20 A; B=1-161.
DR PDBsum; 1KN1; -.
DR AlphaFoldDB; P59857; -.
DR SMR; P59857; -.
DR GeneID; 3978964; -.
DR EvolutionaryTrace; P59857; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR CDD; cd12126; APC_beta; 1.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR006245; ApcB.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR PANTHER; PTHR34011:SF3; PTHR34011:SF3; 1.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01337; apcB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chloroplast; Chromophore;
KW Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW Plastid; Thylakoid; Transport.
FT CHAIN 1..161
FT /note="Allophycocyanin beta chain"
FT /id="PRO_0000199101"
FT BINDING 66
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /evidence="ECO:0000269|PubMed:10358042"
FT BINDING 71
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /evidence="ECO:0000269|PubMed:10358042"
FT BINDING 76
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /evidence="ECO:0000269|PubMed:10358042"
FT BINDING 81..84
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT BINDING 81
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:10358042"
FT MOD_RES 71
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000250"
FT HELIX 4..13
FT /evidence="ECO:0007829|PDB:1KN1"
FT TURN 14..16
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 21..46
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 48..59
FT /evidence="ECO:0007829|PDB:1KN1"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 75..98
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 102..107
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 112..119
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 123..141
FT /evidence="ECO:0007829|PDB:1KN1"
FT HELIX 143..160
FT /evidence="ECO:0007829|PDB:1KN1"
SQ SEQUENCE 161 AA; 17484 MW; C94F17EE5BF694D3 CRC64;
MQDAITSVIN AADVQGKYLD DSSVEKLRGY FQTGELRVRA AATIAANAAT IIKESVAKSL
LYSDITRPGG NMYTTRRYAA CIRDLDYYLR YATYGMLAGD PSILEERVLN GLKETYNSLG
VPIGATIQAI LAMKEVTISL VGPDAGKEMG LYFDYICSGL S
