ID   IF1_BURTA               Reviewed;          72 AA.
AC   Q2SU48;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Translation initiation factor IF-1 {ECO:0000255|HAMAP-Rule:MF_00075};
GN   Name=infA {ECO:0000255|HAMAP-Rule:MF_00075}; OrderedLocusNames=BTH_I3047;
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS   E264).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
RN   [2]
RP   STRUCTURE BY NMR.
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RA   Barnwal R., Varani G.;
RT   "NMR assignments and structure of translation initiation factor IF-1 from
RT   Burkholderia thailandensis E264.";
RL   Submitted (JUN-2015) to the PDB data bank.
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit
CC       to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps
CC       modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).
CC       Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are
CC       released leaving the mature 70S translation initiation complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBUNIT: Component of the 30S ribosomal translation pre-initiation
CC       complex which assembles on the 30S ribosome in the order IF-2 and IF-3,
CC       IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at
CC       any time during PIC assembly. {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SIMILARITY: Belongs to the IF-1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00075}.
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DR   EMBL; CP000086; ABC39541.1; -; Genomic_DNA.
DR   RefSeq; WP_004521905.1; NZ_CP008786.1.
DR   PDB; 2N3S; NMR; -; A=1-72.
DR   PDBsum; 2N3S; -.
DR   AlphaFoldDB; Q2SU48; -.
DR   SMR; Q2SU48; -.
DR   PRIDE; Q2SU48; -.
DR   EnsemblBacteria; ABC39541; ABC39541; BTH_I3047.
DR   GeneID; 60548068; -.
DR   GeneID; 62009766; -.
DR   GeneID; 64462478; -.
DR   GeneID; 67433089; -.
DR   KEGG; bte:BTH_I3047; -.
DR   HOGENOM; CLU_151267_1_0_4; -.
DR   OMA; ECLRSAM; -.
DR   OrthoDB; 2066663at2; -.
DR   Proteomes; UP000001930; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043022; F:ribosome binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04451; S1_IF1; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00075; IF_1; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006196; RNA-binding_domain_S1_IF1.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR004368; TIF_IF1.
DR   PANTHER; PTHR33370; PTHR33370; 1.
DR   Pfam; PF01176; eIF-1a; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00008; infA; 1.
DR   PROSITE; PS50832; S1_IF1_TYPE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Initiation factor; Protein biosynthesis;
KW   RNA-binding; rRNA-binding.
FT   CHAIN           1..72
FT                   /note="Translation initiation factor IF-1"
FT                   /id="PRO_0000263777"
FT   DOMAIN          1..72
FT                   /note="S1-like"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00075"
FT   STRAND          7..17
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   TURN            18..20
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   STRAND          21..25
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   STRAND          27..29
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   STRAND          31..36
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   STRAND          52..58
FT                   /evidence="ECO:0007829|PDB:2N3S"
FT   STRAND          61..70
FT                   /evidence="ECO:0007829|PDB:2N3S"
SQ   SEQUENCE   72 AA;  8218 MW;  983A496214B869D9 CRC64;
     MAKDDVIQMQ GEVIENLPNA TFRVKLENGH VVLGHISGKM RMHYIRILPG DKVTVELTPY
     DLSRARIVFR AK