ID   IF1_CLOD6               Reviewed;          72 AA.
AC   Q18CI2;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Translation initiation factor IF-1 {ECO:0000255|HAMAP-Rule:MF_00075};
GN   Name=infA {ECO:0000255|HAMAP-Rule:MF_00075}; OrderedLocusNames=CD630_00940;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit
CC       to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps
CC       modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).
CC       Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are
CC       released leaving the mature 70S translation initiation complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBUNIT: Component of the 30S ribosomal translation pre-initiation
CC       complex which assembles on the 30S ribosome in the order IF-2 and IF-3,
CC       IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at
CC       any time during PIC assembly. {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SIMILARITY: Belongs to the IF-1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00075}.
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DR   EMBL; AM180355; CAJ66912.1; -; Genomic_DNA.
DR   RefSeq; WP_003421127.1; NZ_CP010905.2.
DR   RefSeq; YP_001086561.1; NC_009089.1.
DR   PDB; 6C00; NMR; -; A=1-72.
DR   PDBsum; 6C00; -.
DR   AlphaFoldDB; Q18CI2; -.
DR   BMRB; Q18CI2; -.
DR   SMR; Q18CI2; -.
DR   STRING; 272563.CD630_00940; -.
DR   EnsemblBacteria; CAJ66912; CAJ66912; CD630_00940.
DR   GeneID; 66352595; -.
DR   KEGG; cdf:CD630_00940; -.
DR   KEGG; pdc:CDIF630_00163; -.
DR   PATRIC; fig|272563.120.peg.103; -.
DR   eggNOG; COG0361; Bacteria.
DR   OMA; AHVSGKM; -.
DR   PhylomeDB; Q18CI2; -.
DR   BioCyc; PDIF272563:G12WB-151-MON; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043022; F:ribosome binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04451; S1_IF1; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00075; IF_1; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006196; RNA-binding_domain_S1_IF1.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR004368; TIF_IF1.
DR   PANTHER; PTHR33370; PTHR33370; 1.
DR   Pfam; PF01176; eIF-1a; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00008; infA; 1.
DR   PROSITE; PS50832; S1_IF1_TYPE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Initiation factor; Protein biosynthesis;
KW   Reference proteome; RNA-binding; rRNA-binding.
FT   CHAIN           1..72
FT                   /note="Translation initiation factor IF-1"
FT                   /id="PRO_0000263785"
FT   DOMAIN          1..72
FT                   /note="S1-like"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00075"
FT   STRAND          7..17
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   TURN            18..20
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   STRAND          21..25
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   STRAND          31..36
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   HELIX           38..42
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   STRAND          52..58
FT                   /evidence="ECO:0007829|PDB:6C00"
FT   STRAND          64..70
FT                   /evidence="ECO:0007829|PDB:6C00"
SQ   SEQUENCE   72 AA;  8282 MW;  FD99A6C3993BB02B CRC64;
     MAKKDVIELE GTVSEALPNA MFKVKLENGH EILCHISGKL RMNFIRILEG DKVNVELSPY
     DLTRGRITWR KK