ID   APCE_AGLNE              Reviewed;         885 AA.
AC   P28561;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Phycobiliprotein ApcE;
DE            EC=4.-.-.-;
DE   AltName: Full=Anchor polypeptide;
DE   AltName: Full=PBS-anchor protein;
DE   AltName: Full=Phycobilisome linker polypeptide;
GN   Name=apcE;
OS   Aglaothamnion neglectum (Red alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Florideophyceae; Rhodymeniophycidae; Ceramiales;
OC   Callithamniaceae; Aglaothamnion.
OX   NCBI_TaxID=2765;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7678762; DOI=10.1007/bf00039615;
RA   Apt K.E., Grossman A.R.;
RT   "Characterization and transcript analysis of the major phycobiliprotein
RT   subunit genes from Aglaothamnion neglectum (Rhodophyta).";
RL   Plant Mol. Biol. 21:27-38(1993).
CC   -!- FUNCTION: This protein is postulated to act both as terminal energy
CC       acceptor and as a linker polypeptide that stabilizes the phycobilisome
CC       architecture. May have intrinsic bilin lyase activity (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Stromal side
CC       {ECO:0000250}.
CC   -!- PTM: Contains one covalently linked bilin chromophore. This protein
CC       autochromophorylates (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phycobilisome linker protein family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00775}.
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DR   EMBL; Z11905; CAA77957.1; -; Genomic_DNA.
DR   PIR; S20621; S20621.
DR   AlphaFoldDB; P28561; -.
DR   SMR; P28561; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3130.20; -; 3.
DR   Gene3D; 1.10.490.20; -; 1.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR001297; PBS_linker_dom.
DR   InterPro; IPR038255; PBS_linker_sf.
DR   InterPro; IPR012128; Phycobilisome_asu/bsu.
DR   InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR   Pfam; PF00427; PBS_linker_poly; 3.
DR   Pfam; PF00502; Phycobilisome; 2.
DR   SUPFAM; SSF46458; SSF46458; 1.
DR   PROSITE; PS51445; PBS_LINKER; 3.
PE   3: Inferred from homology;
KW   Antenna complex; Bile pigment; Chloroplast; Chromophore;
KW   Electron transport; Lyase; Membrane; Photosynthesis; Phycobilisome;
KW   Plastid; Repeat; Thylakoid; Transport.
FT   CHAIN           1..885
FT                   /note="Phycobiliprotein ApcE"
FT                   /id="PRO_0000199259"
FT   DOMAIN          242..422
FT                   /note="PBS-linker 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   DOMAIN          498..680
FT                   /note="PBS-linker 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   DOMAIN          694..871
FT                   /note="PBS-linker 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   BINDING         185
FT                   /ligand="(2R,3E)-phycocyanobilin"
FT                   /ligand_id="ChEBI:CHEBI:85275"
FT                   /note="covalent, via 1 link"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   885 AA;  101154 MW;  C91D75CD6307B4B9 CRC64;
     MIVNASSESP RVQPQLYRTA SISTIVQAEQ QDRFLQLGEL NDLVTFLNSG TKRLEIAEVL
     SKNADILVAK AADKIFVGGS AISYLERPQA SFVEFDNISM TQKEMSGNVQ VNLLSNTSSN
     VISNDTLPPG FKPINVVRYG STRMKKSLRD LDWFLRYLTY AIIAGDPNIL SVNIRGLKDL
     ISNACSSAAA TVALREMRKS AISIFDNDLS AQEIVQQYFN ILITEFDSPS LTDKIRRRTS
     TDVQGLRLPQ IYSKSGIPLQ RFVMKTSLSN EEKSSVLKAC YRQVFERDIA KAYNLSFVDL
     ESQVKNGSLS IKEFIRRIGK SSVYRKQFYE PFVNSRVVEL AFKHFLGRGL SSLEEFQKYF
     SILSLRGLNG LIDALINSLE YADYFAEETC PYLRGLSEEP LESRNWGVQF SLLNYSAPFR
     KVPQFITLFS DYKMSLPDQH PYGLGNDHLA LQFGAIFPNS LTALSNKSAF FGRYTRRILL
     RQGPGIYSQI SNPQARSKSI GSLGPKIFKL NSIDNTYFSD EVMSLPPDIE QIIKAIYLRV
     FGRFIYLEEL SSVRKFESLF RSSKISVRDF IRNLVKSSVF RSLYWEPLYI CKAIEYIHYR
     LLGRPSYGRQ EINQYFDIVY REGYYKMIDY LLNSSEYIRS FGDNTVPYER YITSANMILK
     SNYSNFLYST LKTKNSNSKK FIELSNILEK RSLNSIQARI SQGVSTVRDQ YKVFQLTQLS
     SQVDKRQVVK AIYRQIFERD LNSFAIGDEF INLEKALVNN NITVQQFIEQ IGSSSLYGRE
     FYQPYPNTKV IELGTKHFLG RAPNNQAEIR YYNQILASQG LSSFITVLVN SNEYNQVFGV
     NIVPYRRFTT LPAANFPNTE KLYNTLTKQS SEIIVPSFIV VPGNQ