APCE_AROAE
ID APCE_AROAE Reviewed; 303 AA.
AC Q5P5G6;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Acetophenone carboxylase epsilon subunit;
DE EC=6.4.1.8;
DE AltName: Full=Acetophenone carboxylase 34 kDa subunit;
GN Name=apc5; Synonyms=apcE; OrderedLocusNames=AZOSEA13210; ORFNames=c1A97;
OS Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae; Aromatoleum.
OX NCBI_TaxID=76114;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EbN1;
RX PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA Reinhardt R.;
RT "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT bacterium, strain EbN1.";
RL Arch. Microbiol. 183:27-36(2005).
RN [2]
RP PROTEIN SEQUENCE OF 1-23, AND INDUCTION.
RX PubMed=10941798;
RA Champion K.M., Zengler K., Rabus R.;
RT "Anaerobic degradation of ethylbenzene and toluene in denitrifying strain
RT EbN1 proceeds via independent substrate-induced pathways.";
RL J. Mol. Microbiol. Biotechnol. 1:157-164(1999).
RN [3]
RP IDENTIFICATION.
RX PubMed=12420173; DOI=10.1007/s00203-002-0487-2;
RA Rabus R., Kube M., Beck A., Widdel F., Reinhardt R.;
RT "Genes involved in the anaerobic degradation of ethylbenzene in a
RT denitrifying bacterium, strain EbN1.";
RL Arch. Microbiol. 178:506-516(2002).
RN [4]
RP INDUCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15687214; DOI=10.1128/jb.187.4.1493-1503.2005;
RA Kuhner S., Wohlbrand L., Fritz I., Wruck W., Hultschig C., Hufnagel P.,
RA Kube M., Reinhardt R., Rabus R.;
RT "Substrate-dependent regulation of anaerobic degradation pathways for
RT toluene and ethylbenzene in a denitrifying bacterium, strain EbN1.";
RL J. Bacteriol. 187:1493-1503(2005).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP PROPERTIES, COFACTOR, AND SUBUNIT.
RX PubMed=20047908; DOI=10.1128/jb.01423-09;
RA Jobst B., Schuhle K., Linne U., Heider J.;
RT "ATP-dependent carboxylation of acetophenone by a novel type of
RT carboxylase.";
RL J. Bacteriol. 192:1387-1394(2010).
CC -!- FUNCTION: Catalyzes the carboxylation of acetophenone to form 3-oxo-3-
CC phenylpropanoate (benzoylacetate) in the anaerobic catabolism of
CC ethylbenzene. Also carboxylates propiophenone at the same rate and 4-
CC acetyl-pyridine at lower rates. {ECO:0000269|PubMed:20047908}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetophenone + 2 ATP + H2O + hydrogencarbonate = 3-oxo-3-
CC phenylpropanoate + 2 ADP + 2 H(+) + 2 phosphate;
CC Xref=Rhea:RHEA:28647, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:22731, ChEBI:CHEBI:27632,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=6.4.1.8;
CC Evidence={ECO:0000269|PubMed:20047908};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:20047908};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:20047908};
CC Note=Divalent metal cations. Magnesium or manganese are required for
CC activity. {ECO:0000269|PubMed:20047908};
CC -!- ACTIVITY REGULATION: Inhibited by zinc ions, carbamoylphosphate and
CC beta,gamma-imido-ATP. {ECO:0000269|PubMed:20047908}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=33 uM for acetophone {ECO:0000269|PubMed:20047908};
CC KM=0.54 mM for HCO(3)(-) {ECO:0000269|PubMed:20047908};
CC KM=0.5 mM for ATP {ECO:0000269|PubMed:20047908};
CC Vmax=51 mmol/min/mg enzyme {ECO:0000269|PubMed:20047908};
CC Note=Kinetic parameters have been established using the heteromeric
CC complex including recombinant Apc5.;
CC -!- SUBUNIT: Acetophenone carboxylase consists of five subunits; a
CC heterooctameric subcomplex of two alpha (Apc1), two beta (Apc2), two
CC gamma (Apc3) and two delta (Apc4) subunits assembles with the epsilon
CC (Apc5) subunit in an unknown stoichiometry.
CC {ECO:0000269|PubMed:20047908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By ethylbenzene, toluene and acetophenone.
CC {ECO:0000269|PubMed:10941798, ECO:0000269|PubMed:15687214}.
CC -!- MISCELLANEOUS: The epsilon subunit dissociates during enzyme
CC purification. However, Apc5 is required for acetophenone carboxylation
CC and the enzyme complex is reconstituted using recombinant Apc5.
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DR EMBL; CR555306; CAI07446.1; -; Genomic_DNA.
DR RefSeq; WP_011237166.1; NC_006513.1.
DR AlphaFoldDB; Q5P5G6; -.
DR SMR; Q5P5G6; -.
DR STRING; 76114.c1A97; -.
DR EnsemblBacteria; CAI07446; CAI07446; c1A97.
DR KEGG; eba:c1A97; -.
DR eggNOG; ENOG5033VSK; Bacteria.
DR HOGENOM; CLU_917155_0_0_4; -.
DR OrthoDB; 2152278at2; -.
DR BioCyc; MetaCyc:MON-14364; -.
DR BRENDA; 6.4.1.8; 12182.
DR Proteomes; UP000006552; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Direct protein sequencing; Ligase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..303
FT /note="Acetophenone carboxylase epsilon subunit"
FT /id="PRO_0000419043"
SQ SEQUENCE 303 AA; 32124 MW; 3D04531570BBD979 CRC64;
MEAAGALWRR RMQELARGAG KPHAPLFVPL IMGCAAQIEA IPAIDMVRDG TRLRKNLSEL
RRMLKLDALT CAVPSCMEAE AVGVEVSQDQ WPPRIGTTAQ VDVTAEIDAD RLAASPRIAA
ALDAVRQIAV DPGEPVIAAA LTGPAALVAQ LRAAGVEAGD EAIYDFAGRI LATLARLYAE
AGVNLLSWHE AARPAEEQDD FWKGALGTAG NVARFHRVPP VLVLPASLAA GPWPAQAVPC
PALNHPPLPP VRTHARAWAA DPAGWPCLPV EGVAERLILT DAEVPPETEI ATLKAQVERV
RGE
