ID   APCE_CYACA              Reviewed;         870 AA.
AC   Q9TLS6;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Phycobiliprotein ApcE;
DE            EC=4.-.-.-;
DE   AltName: Full=Anchor polypeptide;
DE   AltName: Full=PBS-anchor protein;
DE   AltName: Full=Phycobilisome linker polypeptide;
GN   Name=apcE;
OS   Cyanidium caldarium (Red alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae; Cyanidium.
OX   NCBI_TaxID=2771;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RK-1;
RX   PubMed=11040290; DOI=10.1007/s002390010101;
RA   Gloeckner G., Rosenthal A., Valentin K.-U.;
RT   "The structure and gene repertoire of an ancient red algal plastid
RT   genome.";
RL   J. Mol. Evol. 51:382-390(2000).
CC   -!- FUNCTION: This protein is postulated to act both as terminal energy
CC       acceptor and as a linker polypeptide that stabilizes the phycobilisome
CC       architecture. May have intrinsic bilin lyase activity (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Stromal side
CC       {ECO:0000250}.
CC   -!- PTM: Contains one covalently linked bilin chromophore. This protein
CC       autochromophorylates (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the phycobilisome linker protein family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00775}.
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DR   EMBL; AF022186; AAF12901.1; -; Genomic_DNA.
DR   RefSeq; NP_045193.1; NC_001840.1.
DR   AlphaFoldDB; Q9TLS6; -.
DR   SMR; Q9TLS6; -.
DR   GeneID; 800215; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3130.20; -; 3.
DR   Gene3D; 1.10.490.20; -; 1.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR001297; PBS_linker_dom.
DR   InterPro; IPR038255; PBS_linker_sf.
DR   InterPro; IPR012128; Phycobilisome_asu/bsu.
DR   InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR   Pfam; PF00427; PBS_linker_poly; 3.
DR   Pfam; PF00502; Phycobilisome; 2.
DR   SUPFAM; SSF46458; SSF46458; 1.
DR   PROSITE; PS51445; PBS_LINKER; 3.
PE   3: Inferred from homology;
KW   Antenna complex; Bile pigment; Chloroplast; Chromophore;
KW   Electron transport; Lyase; Membrane; Photosynthesis; Phycobilisome;
KW   Plastid; Repeat; Thylakoid; Transport.
FT   CHAIN           1..870
FT                   /note="Phycobiliprotein ApcE"
FT                   /id="PRO_0000199260"
FT   DOMAIN          241..421
FT                   /note="PBS-linker 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   DOMAIN          482..665
FT                   /note="PBS-linker 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   DOMAIN          679..856
FT                   /note="PBS-linker 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   BINDING         184
FT                   /ligand="(2R,3E)-phycocyanobilin"
FT                   /ligand_id="ChEBI:CHEBI:85275"
FT                   /note="covalent, via 1 link"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   870 AA;  99077 MW;  7FC11E1AEC6EA2BA CRC64;
     MGLRTSSGSP LVKPKLYKTS ASNVISLAEK QDRFLNLGEL TDLNTYFSSG NRRLDIAKVI
     SLNANLIISR AADRIFVGGS PLSFLERPQA AVTLTSDQAS STSIQSTKGL GNGNIFQNFF
     KSTSEAPTGF KPINVVRYGS SNMKKSIRDL DWFLRYVTYA IVAGDTSILI VNTKGLRELI
     DKACSSSAAI VALKEMKNVS LSLFNYDIES QNIVRLYFNT LVSEFESPAS SSKVRKRNSL
     DLQGLAIPDI YLVAADKSLR YVMKPNLSNT EKAQVIKACY RQVFERDIAK AYGLSLLELE
     SKLKNLQISV KEFIRALGKS TLYRKNFYEG FTNSRVVELA FRHFMGRGLS SLQEFRKYFA
     ILSSNGLDAL IDSIINNSEY AEYFGEETVP YIRGYGQEAQ ECRNWGSQFA LFKYSAPFRT
     IPQFITLFAD YTQLPPSQHC YGKLNDPLNI QFGAIFKNSY VNEQSRPVLF PRGSRRILVY
     KGAGIFNQLG SPNALEKPPS NVSIAKWSKE TDLNFILNAA YLRVFGRYVY EEEKIALRPL
     ENEFKRRSIS VRDFVGQLAK SDVFRSLYWS RLYICKSIEY IHIRLLGRPT YGRTEINNYF
     DIVYKSGFYA FVDSLVNSRE YIKCFGNDTV PYDRYSTPEA VSSSIFRLSF INSVSYKSLK
     PKIEKFIQLG VARDAKSLSS LNSKVFQGVS QARSQKRVFK VSDYSNVMNL RIVFYAALRQ
     VFERNIEPYI KGGEFKDIES LFLSGKISVR ELIKEIGSSS LYRKEFYIPF PNTQVIEFCT
     KHFLGRAPKN QSEIRYYNQV LAVQGLREMI NYMINSKEYL SVFGDDIVPY RRFPTLPAAN
     FPNTQRLYSR QTKQNRNIVV PSFSGLLNTV