ID   IF1_PSEAE               Reviewed;          72 AA.
AC   P65116; Q9I0L9;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Translation initiation factor IF-1 {ECO:0000255|HAMAP-Rule:MF_00075};
GN   Name=infA {ECO:0000255|HAMAP-Rule:MF_00075}; OrderedLocusNames=PA2619;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit
CC       to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps
CC       modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).
CC       Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are
CC       released leaving the mature 70S translation initiation complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBUNIT: Component of the 30S ribosomal translation pre-initiation
CC       complex which assembles on the 30S ribosome in the order IF-2 and IF-3,
CC       IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at
CC       any time during PIC assembly. {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SIMILARITY: Belongs to the IF-1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00075}.
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DR   EMBL; AE004091; AAG06007.1; -; Genomic_DNA.
DR   PIR; A83319; A83319.
DR   RefSeq; NP_251309.1; NC_002516.2.
DR   RefSeq; WP_002553999.1; NZ_QZGE01000008.1.
DR   PDB; 2N78; NMR; -; A=1-72.
DR   PDBsum; 2N78; -.
DR   AlphaFoldDB; P65116; -.
DR   SMR; P65116; -.
DR   STRING; 287.DR97_5343; -.
DR   PaxDb; P65116; -.
DR   PRIDE; P65116; -.
DR   DNASU; 882325; -.
DR   EnsemblBacteria; AAG06007; AAG06007; PA2619.
DR   GeneID; 58767738; -.
DR   GeneID; 61931168; -.
DR   GeneID; 64443124; -.
DR   GeneID; 67381632; -.
DR   GeneID; 882325; -.
DR   KEGG; pae:PA2619; -.
DR   PATRIC; fig|208964.12.peg.2741; -.
DR   PseudoCAP; PA2619; -.
DR   HOGENOM; CLU_151267_1_0_6; -.
DR   InParanoid; P65116; -.
DR   OMA; AHVSGKM; -.
DR   PhylomeDB; P65116; -.
DR   BioCyc; PAER208964:G1FZ6-2659-MON; -.
DR   PRO; PR:P65116; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04451; S1_IF1; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00075; IF_1; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006196; RNA-binding_domain_S1_IF1.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR004368; TIF_IF1.
DR   PANTHER; PTHR33370; PTHR33370; 1.
DR   Pfam; PF01176; eIF-1a; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00008; infA; 1.
DR   PROSITE; PS50832; S1_IF1_TYPE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Initiation factor; Protein biosynthesis;
KW   Reference proteome; RNA-binding; rRNA-binding.
FT   CHAIN           1..72
FT                   /note="Translation initiation factor IF-1"
FT                   /id="PRO_0000095844"
FT   DOMAIN          1..72
FT                   /note="S1-like"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00075"
FT   STRAND          7..13
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   TURN            18..20
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   STRAND          21..26
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   TURN            27..29
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   STRAND          30..34
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   HELIX           38..43
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   STRAND          52..58
FT                   /evidence="ECO:0007829|PDB:2N78"
FT   STRAND          61..71
FT                   /evidence="ECO:0007829|PDB:2N78"
SQ   SEQUENCE   72 AA;  8303 MW;  97E5EEFD8F3886DB CRC64;
     MSKEDSFEME GTVVDTLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG DKVRVELTPY
     DLSKGRITYR AR