ID   IF1_STAA8               Reviewed;          72 AA.
AC   Q2FW28;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Translation initiation factor IF-1 {ECO:0000255|HAMAP-Rule:MF_00075};
GN   Name=infA {ECO:0000255|HAMAP-Rule:MF_00075};
GN   OrderedLocusNames=SAOUHSC_02489;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit
CC       to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps
CC       modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).
CC       Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are
CC       released leaving the mature 70S translation initiation complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBUNIT: Component of the 30S ribosomal translation pre-initiation
CC       complex which assembles on the 30S ribosome in the order IF-2 and IF-3,
CC       IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at
CC       any time during PIC assembly. {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00075}.
CC   -!- SIMILARITY: Belongs to the IF-1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00075}.
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DR   EMBL; CP000253; ABD31508.1; -; Genomic_DNA.
DR   RefSeq; WP_001118443.1; NZ_LS483365.1.
DR   RefSeq; YP_500957.1; NC_007795.1.
DR   PDB; 2N8N; NMR; -; A=1-72.
DR   PDBsum; 2N8N; -.
DR   AlphaFoldDB; Q2FW28; -.
DR   SMR; Q2FW28; -.
DR   STRING; 1280.SAXN108_2478; -.
DR   EnsemblBacteria; ABD31508; ABD31508; SAOUHSC_02489.
DR   GeneID; 3920866; -.
DR   GeneID; 60544568; -.
DR   GeneID; 62692419; -.
DR   GeneID; 64233776; -.
DR   GeneID; 66915042; -.
DR   KEGG; sao:SAOUHSC_02489; -.
DR   PATRIC; fig|93061.5.peg.2245; -.
DR   eggNOG; COG0361; Bacteria.
DR   HOGENOM; CLU_151267_1_0_9; -.
DR   OMA; AHVSGKM; -.
DR   PRO; PR:Q2FW28; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04451; S1_IF1; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00075; IF_1; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006196; RNA-binding_domain_S1_IF1.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR004368; TIF_IF1.
DR   PANTHER; PTHR33370; PTHR33370; 1.
DR   Pfam; PF01176; eIF-1a; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00008; infA; 1.
DR   PROSITE; PS50832; S1_IF1_TYPE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Initiation factor; Protein biosynthesis;
KW   Reference proteome; RNA-binding; rRNA-binding.
FT   CHAIN           1..72
FT                   /note="Translation initiation factor IF-1"
FT                   /id="PRO_0000263877"
FT   DOMAIN          1..72
FT                   /note="S1-like"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00075"
FT   STRAND          7..16
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   STRAND          18..26
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   STRAND          31..36
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   HELIX           38..42
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   STRAND          52..57
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   TURN            59..61
FT                   /evidence="ECO:0007829|PDB:2N8N"
FT   STRAND          62..70
FT                   /evidence="ECO:0007829|PDB:2N8N"
SQ   SEQUENCE   72 AA;  8280 MW;  617FB2CBD59998EF CRC64;
     MAKQDVIELE GTVLDTLPNA MFKVELENGH EILAHVSGKI RMNYIRILPG DKVTVEMSPY
     DLTRGRITYR YK