APD14_APIME
ID APD14_APIME Reviewed; 168 AA.
AC Q06601; P11525; P11526; P11527;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Apidaecins type 14;
DE Contains:
DE RecName: Full=Apidaecin-2;
DE AltName: Full=Apidaecin II;
DE Contains:
DE RecName: Full=Apidaecin-1B;
DE AltName: Full=Apidaecin IB;
DE Contains:
DE RecName: Full=Apidaecin-1A;
DE AltName: Full=Apidaecin IA;
DE Flags: Precursor;
GN Name=APID14;
OS Apis mellifera (Honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7460;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8467807; DOI=10.1002/j.1460-2075.1993.tb05801.x;
RA Casteels-Josson K., Capaci T., Casteels P., Tempst P.;
RT "Apidaecin multipeptide precursor structure: a putative mechanism for
RT amplification of the insect antibacterial response.";
RL EMBO J. 12:1569-1578(1993).
RN [2]
RP PROTEIN SEQUENCE OF 43-60; 71-88; 99-116; 125-142 AND 151-168, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Hemolymph {ECO:0000303|PubMed:8467807};
RX PubMed=2676519; DOI=10.1002/j.1460-2075.1989.tb08368.x;
RA Casteels P., Ampe C., Jacobs F., Vaeck M., Tempst P.;
RT "Apidaecins: antibacterial peptides from honeybees.";
RL EMBO J. 8:2387-2391(1989).
RN [3]
RP FUNCTION OF APIDAECIN-1B, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC TISSUE=Hemolymph {ECO:0000303|PubMed:7929322};
RX PubMed=7929322; DOI=10.1016/s0021-9258(18)47165-7;
RA Casteels P., Romagnolo J., Castle M., Casteels-Josson K.,
RA Erdjument-Bromage H., Tempst P.;
RT "Biodiversity of apidaecin-type peptide antibiotics. Prospects of
RT manipulating the antibacterial spectrum and combating acquired
RT resistance.";
RL J. Biol. Chem. 269:26107-26115(1994).
CC -!- FUNCTION: Apidaecins have bactericidal activity; predominantly against
CC Gram-negative bacteria (PubMed:2676519, PubMed:7929322). They seem to
CC interfere with cell propagation (PubMed:2676519).
CC {ECO:0000269|PubMed:2676519, ECO:0000269|PubMed:7929322}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2676519,
CC ECO:0000269|PubMed:7929322}.
CC -!- MASS SPECTROMETRY: [Apidaecin-1B]: Mass=2110.0; Method=MALDI;
CC Note=Apidaecin-1B.; Evidence={ECO:0000269|PubMed:7929322};
CC -!- SIMILARITY: Belongs to the apidaecin family. {ECO:0000305}.
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DR EMBL; X72575; CAA51167.1; -; mRNA.
DR PIR; S35330; S35330.
DR RefSeq; NP_001011613.1; NM_001011613.1.
DR AlphaFoldDB; Q06601; -.
DR STRING; 7460.GB51306-PA; -.
DR EnsemblMetazoa; NM_001011613; NP_001011613; GeneID_406140.
DR GeneID; 406140; -.
DR KEGG; ame:406140; -.
DR CTD; 406140; -.
DR PhylomeDB; Q06601; -.
DR Proteomes; UP000005203; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR InterPro; IPR004828; Apidaecin.
DR Pfam; PF00807; Apidaecin; 5.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW Direct protein sequencing; Immunity; Innate immunity; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..42
FT /evidence="ECO:0000269|PubMed:2676519"
FT /id="PRO_0000004910"
FT PEPTIDE 43..60
FT /note="Apidaecin-2"
FT /id="PRO_0000004911"
FT PROPEP 63..70
FT /evidence="ECO:0000269|PubMed:2676519"
FT /id="PRO_0000004912"
FT PEPTIDE 71..88
FT /note="Apidaecin-2"
FT /id="PRO_0000004913"
FT PROPEP 91..98
FT /evidence="ECO:0000269|PubMed:2676519"
FT /id="PRO_0000004914"
FT PEPTIDE 99..116
FT /note="Apidaecin-1B"
FT /id="PRO_0000004915"
FT PROPEP 119..124
FT /evidence="ECO:0000269|PubMed:2676519"
FT /id="PRO_0000004916"
FT PEPTIDE 125..142
FT /note="Apidaecin-1B"
FT /id="PRO_0000004917"
FT PROPEP 145..150
FT /evidence="ECO:0000269|PubMed:2676519"
FT /id="PRO_0000004918"
FT PEPTIDE 151..168
FT /note="Apidaecin-1A"
FT /id="PRO_0000004919"
FT REGION 20..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 168 AA; 19380 MW; 594B931254C04A37 CRC64;
MKNFALAILV VTFVVAVFGN TNLDPPTRPA RLRREAKPEA EPGNNRPIYI PQPRPPHPRL
RREAEPKAEP GNNRPIYIPQ PRPPHPRLRR EAESEAEPGN NRPVYIPQPR PPHPRLRREP
EAEPGNNRPV YIPQPRPPHP RLRREPEAEP GNNRPVYIPQ PRPPHPRI