ID   IF2A_PYRHO              Reviewed;         275 AA.
AC   O58655;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Translation initiation factor 2 subunit alpha;
DE   AltName: Full=aIF2-alpha;
DE   AltName: Full=eIF-2-alpha;
GN   Name=eif2a; OrderedLocusNames=PH0961;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: eIF-2 functions in the early steps of protein synthesis by
CC       forming a ternary complex with GTP and initiator tRNA. {ECO:0000250}.
CC   -!- SUBUNIT: Heterotrimer composed of an alpha, a beta and a gamma chain.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eIF-2-alpha family. {ECO:0000305}.
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DR   EMBL; BA000001; BAA30058.1; -; Genomic_DNA.
DR   PIR; D71087; D71087.
DR   RefSeq; WP_010885052.1; NC_000961.1.
DR   PDB; 3AEV; X-ray; 2.80 A; A=1-275.
DR   PDBsum; 3AEV; -.
DR   AlphaFoldDB; O58655; -.
DR   SMR; O58655; -.
DR   STRING; 70601.3257375; -.
DR   EnsemblBacteria; BAA30058; BAA30058; BAA30058.
DR   GeneID; 1443287; -.
DR   KEGG; pho:PH0961; -.
DR   eggNOG; arCOG04107; Archaea.
DR   OMA; DVNEHQR; -.
DR   OrthoDB; 84684at2157; -.
DR   EvolutionaryTrace; O58655; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd04452; S1_IF2_alpha; 1.
DR   Gene3D; 1.10.150.190; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.70.1130; -; 1.
DR   HAMAP; MF_00231; eIF_2_alpha; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR044126; S1_IF2_alpha.
DR   InterPro; IPR022964; TIF2_asu_arc.
DR   InterPro; IPR024055; TIF2_asu_C.
DR   InterPro; IPR024054; TIF2_asu_middle_sf.
DR   InterPro; IPR011488; TIF_2_asu.
DR   PANTHER; PTHR10602; PTHR10602; 1.
DR   Pfam; PF07541; EIF_2_alpha; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF110993; SSF110993; 1.
DR   SUPFAM; SSF116742; SSF116742; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS50126; S1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Initiation factor; Protein biosynthesis; RNA-binding.
FT   CHAIN           1..275
FT                   /note="Translation initiation factor 2 subunit alpha"
FT                   /id="PRO_0000137400"
FT   DOMAIN          12..83
FT                   /note="S1 motif"
FT   STRAND          14..23
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   STRAND          26..31
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   STRAND          38..42
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           43..45
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           54..56
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   STRAND          63..72
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   TURN            73..76
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   STRAND          77..82
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           87..114
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           118..124
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           126..133
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           136..146
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           148..151
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           157..159
FT                   /evidence="ECO:0007829|PDB:3AEV"
FT   HELIX           160..170
FT                   /evidence="ECO:0007829|PDB:3AEV"
SQ   SEQUENCE   275 AA;  31980 MW;  6AAAD15F10FFB436 CRC64;
     MPRKAREYPE EGEFVVATVK RIHNYGAFLE LDEYPGKEAF MHISEVASTW VRNIRDYLKE
     GQKVVAKVIR VDPRKGHIDL SLRRVTQQQR KAKLQEFKRA QKAENLLRLA AEKLGKDFEA
     AWREVWVPLE EEWGEVYAAF EDAAKDGIEV LKGHVPDEWL PVLKEIVENY VEVPTVTIDA
     EFEITVPKPN GVEIIKEALI RARDRANKEK DIEVKFTYQG APRYRIDITA PDYYKAEEVL
     EDIAEEILRV IKQAGGEATL LRKEKRIRKV KKRKK