ID   IF2C_GALSU              Reviewed;         259 AA.
AC   Q08810;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Translation initiation factor IF-2, chloroplastic;
DE   Flags: Fragment;
GN   Name=infB;
OS   Galdieria sulphuraria (Red alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae; Galdieria.
OX   NCBI_TaxID=130081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=14-1-1 / Isolate 107.79/Goettingen;
RX   PubMed=8219057; DOI=10.1007/bf00021420;
RA   Kostrzewa M., Zetsche K.;
RT   "Organization of plastid-encoded ATPase genes and flanking regions
RT   including homologues of infB and tsf in the thermophilic red alga Galdieria
RT   sulphuraria.";
RL   Plant Mol. Biol. 23:67-76(1993).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC       and promotes its binding to the 30S ribosomal subunits. Also involved
CC       in the hydrolysis of GTP during the formation of the 70S ribosomal
CC       complex (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU01059}.
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DR   EMBL; X66698; CAA47239.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q08810; -.
DR   SMR; Q08810; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR006847; IF2_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR015760; TIF_IF2.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF04760; IF2_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Chloroplast; GTP-binding; Initiation factor; Nucleotide-binding; Plastid;
KW   Protein biosynthesis.
FT   CHAIN           1..>259
FT                   /note="Translation initiation factor IF-2, chloroplastic"
FT                   /id="PRO_0000137290"
FT   DOMAIN          171..>259
FT                   /note="tr-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT   BINDING         180..187
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   NON_TER         259
SQ   SEQUENCE   259 AA;  29746 MW;  803DC24390F42EBA CRC64;
     MNKINNNLEF NGNEDEHFIE YYNNKNYKSD LLKANKNNKT KNKKNSNFNF KSIEKNRLNY
     NNKKNKFILN RKAYYNSNEN INCQSDIIYV EENITVNELA SKIGVEATEI LKVLFKKGKL
     LNINQILDLS LLNLISQELK INITTIDKKN NIYINTESNN DQNINDKHVK LRAPIVAVLG
     HVNHGKTSLI EKLIKNDLTK AETGHITQHI GAYEFIIGPK DKKIILLDTP GHEAFESIRQ
     RVLKISDIIL LIIAADEGI